植物功能盐对小鼠急性化学性肝损伤的防护实验研究
|
摘要
目的:采用四氯化碳(CCl_4)诱导的急性化学性肝损伤动物模型,评价3种植物功能盐(竹盐、松盐和梅盐)对试验小鼠急性化学性肝损伤的防护作用。方法:将45只5周龄的C57BL/6健康雄性小鼠适应性喂养7天后随机分成9组,末次给药1h后,模型组和试验组小鼠均腹腔注射0.1%的CCl_4橄榄油溶液,剂量为10mL/kg·bw,正常组给予等体积的橄榄油作为对照。禁食过夜,16h后取血液和肝脏样本,测定血清中天冬氨酸转氨酶(AST)的活性和谷氨酸转氨酶(ALT)的活性;测定肝组织匀浆中丙二醛(MDA)含量以及谷胱甘肽(GSH)、总超氧化物歧化酶(T-SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活力;同时,观察试验小鼠肝组织的病理损伤程度。结果:竹盐、松盐和梅盐均可不同程度地降低血清转氨酶(AST和ALT)的水平,降低肝脏的MDA含量、提高GSH水平及T-SOD活力,并减轻肝脏的病理性损伤。结论:以竹盐为代表的3种植物功能盐对CCl_4诱导的小鼠急性化学性肝损伤均有一定的保护作用。
Objective:The protective effects of three plant functional salts(bamboo salt,pine salt and plum salt)on acute chemical liver injury in mice induced by carbon tetrachloride(CCl_4)are studied.Methods:Forty-five healthy C57 BL/6 male mice are randomly divided into 9 groups after 7 days' adaptive feeding.1 h after the last dosing,the mice in the model group and the experimental group are intraperitoneally injected with 0.1% CCl_4 olive oil solution at a dose of 10 mL/kg·bw,and the normal group with the same volume of olive oil as the control group.All mice are forbidden to eat overnight,and blood and liver samples are taken after 16 h.The activities of alanine transaminase(ALT)and aspartate aminotransferase(AST),the content of malondialdehyde(MDA)and glutathione(GSH)in liver homogenate,and the activities of total superoxide dismutase(T-SOD)and GSH-px in liver homogenate are measured.At the same time,the pathological damage degree of liver tissues in mice are examined.Results:Bamboo salt,pine salt and plum salt could reduce the activities of serum transaminase(AST and ALT),reduce the content of MDA in liver,increase GSH level and SOD activity,and alleviate the pathological damage of liver.Conclusion:Three kinds of plant functional salts all show certain functions on chemical liver injury in mice induced by CCl_4.
Objective:The protective effects of three plant functional salts(bamboo salt,pine salt and plum salt)on acute chemical liver injury in mice induced by carbon tetrachloride(CCl_4)are studied.Methods:Forty-five healthy C57 BL/6 male mice are randomly divided into 9 groups after 7 days' adaptive feeding.1 h after the last dosing,the mice in the model group and the experimental group are intraperitoneally injected with 0.1% CCl_4 olive oil solution at a dose of 10 mL/kg·bw,and the normal group with the same volume of olive oil as the control group.All mice are forbidden to eat overnight,and blood and liver samples are taken after 16 h.The activities of alanine transaminase(ALT)and aspartate aminotransferase(AST),the content of malondialdehyde(MDA)and glutathione(GSH)in liver homogenate,and the activities of total superoxide dismutase(T-SOD)and GSH-px in liver homogenate are measured.At the same time,the pathological damage degree of liver tissues in mice are examined.Results:Bamboo salt,pine salt and plum salt could reduce the activities of serum transaminase(AST and ALT),reduce the content of MDA in liver,increase GSH level and SOD activity,and alleviate the pathological damage of liver.Conclusion:Three kinds of plant functional salts all show certain functions on chemical liver injury in mice induced by CCl_4.
引文
[1]金成燮.高效健康竹盐生产新工艺[Z].国家科技成果,2017.
[2]金成燮.竹盐生产的改进方法:中国,CN103416715A[P].2013.
[3]Zhao X,Song J L,Jung O S,et al.Chemical properties and in vivo gastric protective effects of bamboo salt[J].Food Science and Biotechnology,2014,23(3):895-902.
[4]陈湄沁.竹盐及岩盐的生物学功效研究[D].杭州:浙江大学,2015.
[5]Lee H J,Choi C H.Anti-inflammatory effects of bamboo salt and sodium fluoride in human gingival fibroblasts-an in vitro study[J].The Kaohsiung Journal of Medical Sciences,2015,31(6):303-308.
[6]Nam S Y,Oh H A,Choi Y,et al.Inhibition of IL-32signaling by bamboo salt decreases pro-inflammatory responses in cellular models of allergic rhinitis[J].Journal of Medicinal Food,2014,17(9):939-948.
[7]Zhao X,Qi Y C,Yi R K,et al.Anti-ageing skin effects of Korean bamboo salt on SKH1hairless mice[J].International Journal of Biochemistry&Cell Biology,2018,103:1-13.
[8]Yoou M S,Nam S Y,Yoon K W,et al,Bamboo salt suppresses skin inflammation in mice with 2,4-dinitrofluorobenzeneinduced atopic dermatitis[J].Chinese Journal of Natural Medicines,2018,16(2):97-104.
[9]张英.功能性植物盐及其制备方法:中国,CN 201810812721.0[P].2011.
[10]廖笑玲,吴亮,林宗泽,等.番茄红素抑制内质网应激从而减轻CCl4诱导的小鼠急性肝损伤[J].中国临床药理学与治疗学,2018,23(7):770-775.
[11]Tan C Y,Lai R C,Wong W,et al.Mesenchymal stem cellderived exosomes promote hepatic regeneration in druginduced liver injury models[J].Stem Cell Research&Therapy,2014,5(3):76.
[12]Vinken M,Maes M,Vanhaecke T,et al.Drug-induced inducedliver injury:mechanisms,types and biomarkers[J].Current Medical Chemistry,2013,24(20):3011-3021.
[13]Teo D,Ng P,Tan S,et al.Drug-induced liver injury associated with complementary and alternative medicine:a review of adverse event reports in an Asian community from 2009to2014[J].BMC Complementary and Alternative Medicine,2016,16(7):192.
[14]Stine J,Lewis J.Current and future directions in the treatment and prevention of drug induced liver injury:a systematic review[J].Expert Review of Gastroenterology&Hepatology,2016,10(4):517-36.
[15]谢跃杰,刘葳,张忠明,等.油橄榄叶醇提取物对D-半乳糖胺/脂多糖致小鼠急性肝损伤的保护作用及其作用机制[J].食品工业科技,2018,39(3):315-319.
[16]Jr S R,Shneider B L,Bucuvalas J,et al.Acute liver failure in children:the first 348patients in the pediat-ricacute liver failure study group[J].Journal of Pediatrics,2006,148(5):652-658.
[17]黄继汉,黄晓晖,陈志扬,等.药理试验中动物间和动物与人体间的等效剂量换算[J].中国临床药理学与治疗学,2004(9):1069-1072.
[18]朱安妮,李蕊,刘三海,等.四氯化碳诱导小鼠急性肝损伤模型的建立和优化[J].中国肝脏病杂志(电子版),2014,6(1):27-31.
[19]吴雅茗,唐灵芝,翁乐斌,等.红茶菌对四氯化碳致急性肝损伤小鼠的保护作用[J].西北药学杂志,2017,32(2):175-180.
[20]Zhao X,Song J L,Kil J H,et al.Bamboo salt attenuates CCl4-induced hepatic damage in Sprague-Dawley rats[J].Nutrition Research and Practice,2013,7(4):273-280.
[21]骞宇,赵欣.竹盐的碱性特性和抗氧化效果[J].中国调味品,2014,39(2):28-31.
[22]岩濑荣一.盐及其化学[M].北京:轻工业出版社,1960:63-67.
[2]金成燮.竹盐生产的改进方法:中国,CN103416715A[P].2013.
[3]Zhao X,Song J L,Jung O S,et al.Chemical properties and in vivo gastric protective effects of bamboo salt[J].Food Science and Biotechnology,2014,23(3):895-902.
[4]陈湄沁.竹盐及岩盐的生物学功效研究[D].杭州:浙江大学,2015.
[5]Lee H J,Choi C H.Anti-inflammatory effects of bamboo salt and sodium fluoride in human gingival fibroblasts-an in vitro study[J].The Kaohsiung Journal of Medical Sciences,2015,31(6):303-308.
[6]Nam S Y,Oh H A,Choi Y,et al.Inhibition of IL-32signaling by bamboo salt decreases pro-inflammatory responses in cellular models of allergic rhinitis[J].Journal of Medicinal Food,2014,17(9):939-948.
[7]Zhao X,Qi Y C,Yi R K,et al.Anti-ageing skin effects of Korean bamboo salt on SKH1hairless mice[J].International Journal of Biochemistry&Cell Biology,2018,103:1-13.
[8]Yoou M S,Nam S Y,Yoon K W,et al,Bamboo salt suppresses skin inflammation in mice with 2,4-dinitrofluorobenzeneinduced atopic dermatitis[J].Chinese Journal of Natural Medicines,2018,16(2):97-104.
[9]张英.功能性植物盐及其制备方法:中国,CN 201810812721.0[P].2011.
[10]廖笑玲,吴亮,林宗泽,等.番茄红素抑制内质网应激从而减轻CCl4诱导的小鼠急性肝损伤[J].中国临床药理学与治疗学,2018,23(7):770-775.
[11]Tan C Y,Lai R C,Wong W,et al.Mesenchymal stem cellderived exosomes promote hepatic regeneration in druginduced liver injury models[J].Stem Cell Research&Therapy,2014,5(3):76.
[12]Vinken M,Maes M,Vanhaecke T,et al.Drug-induced inducedliver injury:mechanisms,types and biomarkers[J].Current Medical Chemistry,2013,24(20):3011-3021.
[13]Teo D,Ng P,Tan S,et al.Drug-induced liver injury associated with complementary and alternative medicine:a review of adverse event reports in an Asian community from 2009to2014[J].BMC Complementary and Alternative Medicine,2016,16(7):192.
[14]Stine J,Lewis J.Current and future directions in the treatment and prevention of drug induced liver injury:a systematic review[J].Expert Review of Gastroenterology&Hepatology,2016,10(4):517-36.
[15]谢跃杰,刘葳,张忠明,等.油橄榄叶醇提取物对D-半乳糖胺/脂多糖致小鼠急性肝损伤的保护作用及其作用机制[J].食品工业科技,2018,39(3):315-319.
[16]Jr S R,Shneider B L,Bucuvalas J,et al.Acute liver failure in children:the first 348patients in the pediat-ricacute liver failure study group[J].Journal of Pediatrics,2006,148(5):652-658.
[17]黄继汉,黄晓晖,陈志扬,等.药理试验中动物间和动物与人体间的等效剂量换算[J].中国临床药理学与治疗学,2004(9):1069-1072.
[18]朱安妮,李蕊,刘三海,等.四氯化碳诱导小鼠急性肝损伤模型的建立和优化[J].中国肝脏病杂志(电子版),2014,6(1):27-31.
[19]吴雅茗,唐灵芝,翁乐斌,等.红茶菌对四氯化碳致急性肝损伤小鼠的保护作用[J].西北药学杂志,2017,32(2):175-180.
[20]Zhao X,Song J L,Kil J H,et al.Bamboo salt attenuates CCl4-induced hepatic damage in Sprague-Dawley rats[J].Nutrition Research and Practice,2013,7(4):273-280.
[21]骞宇,赵欣.竹盐的碱性特性和抗氧化效果[J].中国调味品,2014,39(2):28-31.
[22]岩濑荣一.盐及其化学[M].北京:轻工业出版社,1960:63-67.