薏苡仁酸败度检查法和限度值的考察
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摘要
目的通过测定药食两用中药薏苡仁酸败度的酸值、羰基值及过氧化值,比较不同贮藏条件下薏苡仁酸败度的差异,并制定薏苡仁酸败度测定的质量限度指标。方法参照中国药典2015年版第四部通则中酸败度测定法测定,测定结果采用聚类分析与经验判别相关性分析,初步拟定薏苡仁酸败度的限度值,并用12批合格饮片对拟定的限度值进行验证。结果所测定的10批样品的酸败度差异较大,且酸败程度与外观性状及贮藏条件均具有一定的相关性,拟定的限度值验证合理且数值服从正态分布。结论拟定以酸值≤30.0,羰基值≤32.0,过氧化值≤0.035作为薏苡仁酸败度测定的质量限度指标,本方法重现性高,操作简单,可用于薏苡仁的质量控制。
OBJECTIVE To determine the acid value, carbonyl value and peroxidation value of the degree of rancidity in the Chinese medicine Coicis Semen which was for medicine and food dual-use. To compare the differences in the acidity of Coicis Semen under different storage conditions, and establish the quality limit indicators for determination of rancidity of Coicis Semen. METHODS According to the fourth section of the Chinese Pharmacopoeia 2015 edition, the determination of acidity was determined by clustering analysis and empirical discriminant correlation analysis. The limit value of the rancidity of the Coicis Semen was preliminarily determined, and the proposed limit value was verified by 12 batches of qualified pieces.RESULTS The rancidity of the 10 batches of samples tested was quite different, and the degree of rancidity had a certain correlation with the appearance and storage conditions. The proposed limits were verified reasonably and the values were subject to normal distribution. CONCLUSION It is proposed that the acid value must ≤30.0, the carbonyl value must ≤32.0, and the peroxide value must ≤0.035 as the quality limit indicator for the determination of rancidity. This method has high reproducibility and simple operation, and can be used for quality control of Coicis Semen.
OBJECTIVE To determine the acid value, carbonyl value and peroxidation value of the degree of rancidity in the Chinese medicine Coicis Semen which was for medicine and food dual-use. To compare the differences in the acidity of Coicis Semen under different storage conditions, and establish the quality limit indicators for determination of rancidity of Coicis Semen. METHODS According to the fourth section of the Chinese Pharmacopoeia 2015 edition, the determination of acidity was determined by clustering analysis and empirical discriminant correlation analysis. The limit value of the rancidity of the Coicis Semen was preliminarily determined, and the proposed limit value was verified by 12 batches of qualified pieces.RESULTS The rancidity of the 10 batches of samples tested was quite different, and the degree of rancidity had a certain correlation with the appearance and storage conditions. The proposed limits were verified reasonably and the values were subject to normal distribution. CONCLUSION It is proposed that the acid value must ≤30.0, the carbonyl value must ≤32.0, and the peroxide value must ≤0.035 as the quality limit indicator for the determination of rancidity. This method has high reproducibility and simple operation, and can be used for quality control of Coicis Semen.
引文
[1]中国药典.四部[S].2015:204.
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[2]巩晓杰,滕建业.药食两用中药薏苡仁研究进展[J].亚太传统医药,2013,9(8):74-75.
[3]张明发,沈雅琴.薏苡仁油抗肺癌药理研究进展[J].世界中医药,2012,7(1):87-89.
[4]杜萌,丁安伟,陈彦.薏苡仁化学成分及其防治肿瘤作用机制研究[J].吉林中医药,2012,32(2):195-198.
[5]HE W H,WAN W X.Methods to control oxidative rancidity of poly unsaturated fatty acid[J].China Feed(中国饲料),2001(15):29-30.
[6]哈喇味的食品有毒[J].新疆农垦科技,2015(4):78.
[7]SHEN X F,FANG L,WANG R W.Research on quality control of rancid sample in rape pollen raw material and its preparations[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(3):308-309.
[8]QIU H X.Discussion on determination method of carbonyl group value in rancidity test of Chinese Pharmacopoeia[J].Chin Pharm Affairs(中国药事),1998,12(2):98.