索氏提取与水蒸气蒸馏提取对丁香油抗氧化及抑制LDL氧化能力的影响
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摘要
采用分光光度法和荧光分析法对这2种丁香油的抗氧化活性及抑制LDL氧化修饰能力进行了比较。结果表明,水蒸气蒸馏丁香油(COESD)的DPPH自由基清除率、总还原力、总抗氧化能力及FRAP法抗氧化能力均显著强于索式提取的(COESM)(P<0.05或P<0.01);通过对LDL氧化过程中Trp荧光淬灭、脂褐素及总荧光产生量、MDA修饰Lys残基荧光变化和全波长扫描比较,COESD的抑制能力也强于COESM的;有效成分含量分析表明COESD比COESM含有更高的抗氧化活性物质总多酚及总黄酮。
The comparison of antioxidant activity and inhibition efficiency on LDL oxidation of two kinds of clove oil were studied by spectrophotometry and fluorescence analysis in this study.The results showed as follows.The scavenging ability on DPPH radicals,total reducing and antioxidant capacities,ferric reducing antioxidant power of COESD were significantly stronger than those of COESM(P<0.05 or P<0.01).During LDL oxidation,the inhibition effect of COESD on tryptophan(Trp)fluorescence quenching,the formation of total fluorescence products and lipofuscins,lysine(Lys)modification detected by MDA and UV-Visible scanning spectra were also better than those of COESM.The results of active component content analyses showed that COESD contained more total polyphenols and flavonoids than COESM.This study provided the reference for further research and development of clove oil.
The comparison of antioxidant activity and inhibition efficiency on LDL oxidation of two kinds of clove oil were studied by spectrophotometry and fluorescence analysis in this study.The results showed as follows.The scavenging ability on DPPH radicals,total reducing and antioxidant capacities,ferric reducing antioxidant power of COESD were significantly stronger than those of COESM(P<0.05 or P<0.01).During LDL oxidation,the inhibition effect of COESD on tryptophan(Trp)fluorescence quenching,the formation of total fluorescence products and lipofuscins,lysine(Lys)modification detected by MDA and UV-Visible scanning spectra were also better than those of COESM.The results of active component content analyses showed that COESD contained more total polyphenols and flavonoids than COESM.This study provided the reference for further research and development of clove oil.
引文
[1]刘龙秀,张小霞,曲文娟,等.丁香不同极性部位抑制低密度脂蛋白脂质氧化修饰的研究[J].食品工业科技,2017,38(22):71-75.
[2]万严,杨琼玉,曲文娟,等.丁香乙酸乙酯相抑制LDL中赖氨酸、色氨酸氧化修饰的研究[J].食品工业科技,2017,38(17):10-16.
[3]刘晓丽,钟少枢,于泓鹏,等.微波法和水蒸气蒸馏法提取丁香精油的研究[J].食品与机械,2012,28(4):110-112.
[4]沈勇根,蔡志鹏,江慎华,等.丁香非挥发性成分抗氧化活性[J].农业机械学报,2012,43(10):131-137.
[5]HADI S.Pengambilan Minyak Atsiri Bunga Cengkeh(Clove Oil)Menggunakan Pelarut N-Heksana Dan Benzena[J].Jurnal Bahan Alam Terbarukan,2013,1(2):25-30.
[6]梁蓉,钟芳,王浩,等.丁香油微乳的制备及抑菌活性研究[J].食品与机械,2016,32(7):127-131.
[7]刘宁,桂云云,刘佳丽.植物中精油提取方法的研究进展及应用现状[J].安徽化工,2013,39(1):26-28.
[8]GUAN Wen-qiang,LI Shu-fen,YAN Rui-xiang,et al.Comparison of essential oils of clove buds extracted with supercritical carbon dioxide and other three traditional extraction methods[J].Food Chemistry,2007,101(4):1 558-1 564.
[9]KOLLMANNSBERGER H,NITZ S,ERTA爦M,et al.Chemical composition and content of essential oil from the bud of cultivated Turkish clove[J].Bioresources,2007,2(2):111-120.
[10]刘仁绿,肖敏,江卫青,等.诃子粗提物及不同极性部位抑制低密度脂蛋白氧化修饰的研究[J].食品工业科技,2013,34(16):100-104.
[11]董学艳,姜铁民,刘继超,等.高通量快速检测母乳总蛋白、乳清蛋白和酪蛋白含量方法的比较研究[J].食品工业科技,2016,37(5):308-310.
[12]岳晓霞,张根生,李志,等.水蒸气蒸馏提取丁香精油及其抑菌性研究[J].中国调味品,2008,33(9):49-52.
[13]LI Jing,WU Hai-qiang,LIU Zhi-gang.Acaricidal activity of clove bud oil against Dermatophagoides farinae(Acari:Pyroglyphidae)[J].Chinese Journal of Parasitology&Parasitic Diseases,2009,27(6):492-493,497.
[14]江慎华,蔡志鹏,廖亮,等.丁香抗氧化活性物质提取及人工胃肠液对其活性的影响[J].农业机械学报,2012,43(7):149-155.
[15]刘梦莹,江慎华,曲文娟,等.恒温超声辅助提取诃子FRAP类抗氧化活性物质的研究[J].食品工业科技,2015,36(20):238-243.
[16]熊一凡,李文,曲文娟,等.恒温超声辅助提取丁香总多酚工艺优化及其对低密度脂蛋白氧化修饰过程中荧光的抑制[J].食品工业科技,2017,38(16):159-166.
[17]CHEN C Y,MILBURY P E,CHUNG S K,et al.Effect of almond skin polyphenolics and quercetin on human LDL and apolipoprotein B-100oxidation and conformation[J].Journal of Nutritional Biochemistry,2007,18(12):785-794.
[18]YANG Qiong-yu,WAN Yan,JIANG Shen-hua,et al.The antioxidant inhibition of clove effective fraction on lipid,protein and spectra variation of LDL[J].Spectroscopy and Spectral Analysis,2017,37(1):312-320.
[19]COMINACINI L,GARBIN U,DAVOLI A,et al.A simple test for predisposition to LDL oxidation based on the fluorescence development during copper-catalyzed oxidative modification[J].Journal of Lipid Research,1991,32(2):349-358.
[20]PICARD S,PARTHASARATHY S,FRUEBIS J,et al.Aminoguanidine inhibits oxidative modification of low density lipoprotein protein and the subsequent increase in uptake by macrophage scavenger receptors[J].Proceedings of the National Academy of Sciences of the United States of America,1992,89(15):6 876-6 880.
[21]ESTERBAUER H,ZOLLERN H.Methods for determination of aldehydic lipid peroxidation products[J].Free Radical Biology&Medicine,1989,7(2):197-203.
[22]江慎华,王书源,马海乐,等.丁香活性物质提取工艺优化与抗氧化活性研究[J].农业机械学报,2010,41(1):132-138.
[23]庄军辉.丁香酚体内外抗氧化作用及其机制研究[D].洛阳:河南科技大学,2014:20.
[24]SUGANTHY N,NISHA S A,PANDIAN S K,et al.E-valuation of gelidiella acerosa,the red algae inhabiting South Indian coastal area for antioxidant and metal chelating potential[J].Biomedicine&Preventive Nutrition,2013,3(4):399-406.
[25]赵楠楠,朱晓冉,李德海.红松壳多酚、黄酮和多糖含量及抗氧化活性相关性的研究[J].现代食品科技,2017(12):44-49.
[26]郭娟.芦丁、丹参素体外抗氧化作用的研究[D].西安:陕西师范大学,2009:23-24.
[27]OLSZOWY Ma?gorzata,DAWIDOWICZ Andrzej L.Essential oils as antioxidants:their evaluation by DPPH,ABTS,FRAP,CUPRAC,andβ-carotene bleaching methods[J].Monatshefte für Chemie-Chemical Monthly,2016,147(12):1-9.
[28]LIU Ting-ting,ZENG Yi,TANG Kun,et al.Dihydromyricetin ameliorates atherosclerosis in LDL receptor deficient mice[J].Atherosclerosis,2017,262:39-50.
[29]CHANDRASEKARA A,SHAHIDI F.Antioxidant Phenolics of Millet Control Lipid Peroxidation in Human LDL Cholesterol and Food Systems[J].Journal of the American Oil Chemists Society,2012,89(2):275-285.
[30]王丽丽,赵新淮.紫丁香叶提取物对低密度脂蛋白氧化修饰的抑制作用[J].东北农业大学学报,2007,38(5):641-644.
[31]谢志勇,谢丽琴,江慎华,等.基于光谱学技术对丁香油与乙酸乙酯相抑制LDL非酶糖基化、氧化修饰能力的比较[J].光谱学与光谱分析,2018,38(2):518-527.
[32]ANTHONY K K,GEORGE D S,SINGH H K B,et al.Reactive Oxygen Species Activity and Antioxidant Properties of Fusarium Infected Bananas[J].Journal of Phytopathology,2017,165(4):213-222.
[2]万严,杨琼玉,曲文娟,等.丁香乙酸乙酯相抑制LDL中赖氨酸、色氨酸氧化修饰的研究[J].食品工业科技,2017,38(17):10-16.
[3]刘晓丽,钟少枢,于泓鹏,等.微波法和水蒸气蒸馏法提取丁香精油的研究[J].食品与机械,2012,28(4):110-112.
[4]沈勇根,蔡志鹏,江慎华,等.丁香非挥发性成分抗氧化活性[J].农业机械学报,2012,43(10):131-137.
[5]HADI S.Pengambilan Minyak Atsiri Bunga Cengkeh(Clove Oil)Menggunakan Pelarut N-Heksana Dan Benzena[J].Jurnal Bahan Alam Terbarukan,2013,1(2):25-30.
[6]梁蓉,钟芳,王浩,等.丁香油微乳的制备及抑菌活性研究[J].食品与机械,2016,32(7):127-131.
[7]刘宁,桂云云,刘佳丽.植物中精油提取方法的研究进展及应用现状[J].安徽化工,2013,39(1):26-28.
[8]GUAN Wen-qiang,LI Shu-fen,YAN Rui-xiang,et al.Comparison of essential oils of clove buds extracted with supercritical carbon dioxide and other three traditional extraction methods[J].Food Chemistry,2007,101(4):1 558-1 564.
[9]KOLLMANNSBERGER H,NITZ S,ERTA爦M,et al.Chemical composition and content of essential oil from the bud of cultivated Turkish clove[J].Bioresources,2007,2(2):111-120.
[10]刘仁绿,肖敏,江卫青,等.诃子粗提物及不同极性部位抑制低密度脂蛋白氧化修饰的研究[J].食品工业科技,2013,34(16):100-104.
[11]董学艳,姜铁民,刘继超,等.高通量快速检测母乳总蛋白、乳清蛋白和酪蛋白含量方法的比较研究[J].食品工业科技,2016,37(5):308-310.
[12]岳晓霞,张根生,李志,等.水蒸气蒸馏提取丁香精油及其抑菌性研究[J].中国调味品,2008,33(9):49-52.
[13]LI Jing,WU Hai-qiang,LIU Zhi-gang.Acaricidal activity of clove bud oil against Dermatophagoides farinae(Acari:Pyroglyphidae)[J].Chinese Journal of Parasitology&Parasitic Diseases,2009,27(6):492-493,497.
[14]江慎华,蔡志鹏,廖亮,等.丁香抗氧化活性物质提取及人工胃肠液对其活性的影响[J].农业机械学报,2012,43(7):149-155.
[15]刘梦莹,江慎华,曲文娟,等.恒温超声辅助提取诃子FRAP类抗氧化活性物质的研究[J].食品工业科技,2015,36(20):238-243.
[16]熊一凡,李文,曲文娟,等.恒温超声辅助提取丁香总多酚工艺优化及其对低密度脂蛋白氧化修饰过程中荧光的抑制[J].食品工业科技,2017,38(16):159-166.
[17]CHEN C Y,MILBURY P E,CHUNG S K,et al.Effect of almond skin polyphenolics and quercetin on human LDL and apolipoprotein B-100oxidation and conformation[J].Journal of Nutritional Biochemistry,2007,18(12):785-794.
[18]YANG Qiong-yu,WAN Yan,JIANG Shen-hua,et al.The antioxidant inhibition of clove effective fraction on lipid,protein and spectra variation of LDL[J].Spectroscopy and Spectral Analysis,2017,37(1):312-320.
[19]COMINACINI L,GARBIN U,DAVOLI A,et al.A simple test for predisposition to LDL oxidation based on the fluorescence development during copper-catalyzed oxidative modification[J].Journal of Lipid Research,1991,32(2):349-358.
[20]PICARD S,PARTHASARATHY S,FRUEBIS J,et al.Aminoguanidine inhibits oxidative modification of low density lipoprotein protein and the subsequent increase in uptake by macrophage scavenger receptors[J].Proceedings of the National Academy of Sciences of the United States of America,1992,89(15):6 876-6 880.
[21]ESTERBAUER H,ZOLLERN H.Methods for determination of aldehydic lipid peroxidation products[J].Free Radical Biology&Medicine,1989,7(2):197-203.
[22]江慎华,王书源,马海乐,等.丁香活性物质提取工艺优化与抗氧化活性研究[J].农业机械学报,2010,41(1):132-138.
[23]庄军辉.丁香酚体内外抗氧化作用及其机制研究[D].洛阳:河南科技大学,2014:20.
[24]SUGANTHY N,NISHA S A,PANDIAN S K,et al.E-valuation of gelidiella acerosa,the red algae inhabiting South Indian coastal area for antioxidant and metal chelating potential[J].Biomedicine&Preventive Nutrition,2013,3(4):399-406.
[25]赵楠楠,朱晓冉,李德海.红松壳多酚、黄酮和多糖含量及抗氧化活性相关性的研究[J].现代食品科技,2017(12):44-49.
[26]郭娟.芦丁、丹参素体外抗氧化作用的研究[D].西安:陕西师范大学,2009:23-24.
[27]OLSZOWY Ma?gorzata,DAWIDOWICZ Andrzej L.Essential oils as antioxidants:their evaluation by DPPH,ABTS,FRAP,CUPRAC,andβ-carotene bleaching methods[J].Monatshefte für Chemie-Chemical Monthly,2016,147(12):1-9.
[28]LIU Ting-ting,ZENG Yi,TANG Kun,et al.Dihydromyricetin ameliorates atherosclerosis in LDL receptor deficient mice[J].Atherosclerosis,2017,262:39-50.
[29]CHANDRASEKARA A,SHAHIDI F.Antioxidant Phenolics of Millet Control Lipid Peroxidation in Human LDL Cholesterol and Food Systems[J].Journal of the American Oil Chemists Society,2012,89(2):275-285.
[30]王丽丽,赵新淮.紫丁香叶提取物对低密度脂蛋白氧化修饰的抑制作用[J].东北农业大学学报,2007,38(5):641-644.
[31]谢志勇,谢丽琴,江慎华,等.基于光谱学技术对丁香油与乙酸乙酯相抑制LDL非酶糖基化、氧化修饰能力的比较[J].光谱学与光谱分析,2018,38(2):518-527.
[32]ANTHONY K K,GEORGE D S,SINGH H K B,et al.Reactive Oxygen Species Activity and Antioxidant Properties of Fusarium Infected Bananas[J].Journal of Phytopathology,2017,165(4):213-222.