牛血清白蛋白调制下金纳米片矿化
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摘要
以牛血清白蛋白(BSA)单分子膜做模板,在室温下通过和亚相中的四氯金酸离子的相互作用,获得了金纳米片.利用透射电镜(TEM),选区电子衍射(SAED)和X射线衍射(XRD)的方法对获得样品进行了表征.结果表明牛血清白蛋白在形成金纳米片的过程起着还原金离子和控制金纳米片取向聚集和生长的双重作用.在反应的初期,获得了无定形态的金纳米颗粒,随着反应时间的推进获得了取向生长的金纳米片.结果表明三角片状的金颗粒沿着(111)面取向生长.该研究对于利用生物路线获取单晶金纳米颗粒提供了一个可以借鉴的方法.
Gold nanoparticles were synthesized by the interaction of the HAu Cl4 subphase with the bovine serum albumin( BSA) monolayers at room temperature. The samples were characterized by transmission electron microscopy( TEM),selected area electron diffraction( SAED) and X ray diffraction( XRD). The results showed that bovine serum albumin played a dual role in the reduction of gold ions and the control of the orientation and growth of gold nanoparticles. In the initial stage of the reaction,the gold nanoparticles with no definite morphology were obtained.Furthermore,the gold nanoparticles with oriented growth were obtained with the reaction time. The results show that the triangular gold particles are oriented along the( 111) plane. This study provides a reference for the use of biological routes to obtain single crystal gold nanoparticles.
Gold nanoparticles were synthesized by the interaction of the HAu Cl4 subphase with the bovine serum albumin( BSA) monolayers at room temperature. The samples were characterized by transmission electron microscopy( TEM),selected area electron diffraction( SAED) and X ray diffraction( XRD). The results showed that bovine serum albumin played a dual role in the reduction of gold ions and the control of the orientation and growth of gold nanoparticles. In the initial stage of the reaction,the gold nanoparticles with no definite morphology were obtained.Furthermore,the gold nanoparticles with oriented growth were obtained with the reaction time. The results show that the triangular gold particles are oriented along the( 111) plane. This study provides a reference for the use of biological routes to obtain single crystal gold nanoparticles.
引文
[1]Mann S.Biomimetic Materials Chemistry[M].Published by Jone Wiley&Sons Inc.,1996.
[2]Boskey A D.Mineralization of bones and teeth[M].Elements,1998(3):385-391.
[3]Marin F,Corstjens P,de Gaulejac B,de Vrind E,et al.Mucins and molluscan calcification.Molecular characterization of mucoperlin,a novel mucin-like protein from the nacreous shell layer of the fan mussel Pinna nobilis[J].J.Biol Chem.,2000,275:20667-20675.
[4]Kaplan D L.Mollusc shell structures:novel design strategies for synthetic materials[J].Curr Opin Solid State Mater.Sci.,1998,3:232-236.
[5]Wetherbee R.The diatom glasshouse[J].Science,2002,298:547-547.
[6]Chen C L,Zhang P J,Rosi N L.Hierarchical self-assembly is a fundamental principle in nature[J].J.Am.Chem.Soc.,2008,130:13555-13557.
[7]Cha J N,Stucky G D,Morse D E,Deming T J.Biomimetic synthesis of ordered silica structures mediated by block copolypeptides[J].Nature,2000,403:289-292.
[8]Raveendran P,Fu J,Wallen S L.Completely“Green”synthesis metalnanoparticles[J].J.Am.Chem.Soc.,2003,125:13940-13941.
[9]Liu B,Xie J,Lee J Y,Ting Y P,et al.Optimization of high-yield biological synthesis of single-crystalline gold nanoplates[J].J.Phys.Chem.B,2005,109:15256-15263.
[10]Raveendran P,Fu J,Wallen S L.A simple and“green”method for the synthesis of Au,Ag,and Au-Ag alloy nanoparticles[J].Green Chem,2006,8:34-38.
[11]Douglas T.A Bright Bio-Inspired Future[J].Science,2003,299:1192-1193.
[12]Yang T,Li Z,Wang L,Guo C L,et al.Synthesis,Characterization and Self-Assembly of Protein Lysozyme Monolayer-Stabilized Gold Nanoparticles[J].Langmuir,2007,23:10533-10538.
[13]Nayak N C,Shin K.Human serum albumin mediated self-assembly of gold nanoparticles into hollowspheres[J].Nanotechnology,2008,19:265603-265603.
[14]Bai X T,Zheng L Q,Li N,Dong B,et al.Synthesis and characterization of microscale gold nanoplates using Langmuir monolayers of long-chain ionic liquid[J].Cryst.Growth Des,2008,8(10):3840-3846.
[15]Sánchez-González J,Cabrerizo-Vílchez M A,Gálvez-Ruiz M J.Evaluation of the interactions between lipids andγ-globulin protein at the air-liquid interface[J].Colloids Surf.B,1999,12:123-138.
[16]Carter D C,Ho J X.Structure of serum albumin[J].Adv.Protein Chem.,1994,45:153-203.
[17]Hirayama K,Akashi S,Furuya M,Fukuhara K L.Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and frit-FAB LC/MS[J].Biochem.Biophys.Res.Commun.,1990,173:639-646.
[18]He X M,Carter D C.Atomic Structure and Chemistry of Human Serum Albumin[J].Nature,1992,358:209-215.
[19](a)Bhargava S K,Booth J M,Agrawal S,Coloe P,et al.Gold Nanoparticle Formation during Bromoaurate Reduction by Amino Acids[J].Langmuir,2005,21:5949-5956.(b)Shao Y,Jin YD,et al.Chem.Commun,2004,9:1104-1105.
[20]Xie J P,Lee J Y,Wang D I C.Protein-aided formation of triangular silver nanoprisms with enhanced SERS performance[J].J.Phys.Chem C,2007,111:10226-10232.
[21]Spadavecchia J,Prete P,Lovergine N,Tapfer L,et al.Au Nanoparticles Prepared by Physical Method on Si and Sapphire Substrates for Biosensor Applications[J].J.Phys.Chem.B,2005,109:17347-17349.
[22]Burt J L,Gutierrez-Wing C,Miki-Yoshida M,et al.Noble-Metal Nanoparticles Directly Conjugated to Globular Proteins[J].Langmuir,2004,20:11778-11783.
[23]De Oliveira D B,Laursen R A.Control of Calcite Crystal Morphology by a Peptide Designed To Bind to a Specific Surface[J].J.Am.Chem.Soc.,1997,119:10627-10631.
[24]Lu J R,Su T J,Thomas R K.Structural Conformation of Bovine Serum Albumin Layers at the Air-Water Interface Studied by Neutron Reflection[J].J.Colloid Interface Sci.,1999,213:426-437.
[25]Auduc N,Ringenbach A,Stevenson I,Jugnet Y,et al.Atomic force microscopy characterization of poly(amino acid)LangmuirBlodgett films[J].Langmuir,1993,9:3567-3573.
[26]Xue Z H,(B)Hu B,Jia X L,et al.Effect of the interaction between bovine serum albumin Langmuir monolayer and calcite on the crystallization of Ca CO3nanoparticles[J].Mater.Chem.Phys.,2009,114:47-52
[27]Kmetko J,Yu C J,Evmenenko G,Kewalramani S,et al.Evidence of Registry at the Interface during Inorganic Nucleation at an Organic Template[J].Phys.Rev.Lett.,2002,89:186102(1)-186102(4).
[28]Popescu D C,Smulders M M J,Pichon B P,et al.Template Adaptability Is Key in the Oriented Crystallization of Ca CO3[J].J.Am.Chem.Soc.,2007,129:14058-14059.
[29]Di Masi E,Kwak S Y,Pichon B P,et al.Structural adaptability in an organic template for Ca CO3 mineralization[J].Cryst.Eng.Comm.,2007,9:1192-1204.
[30]Metzler R A,Kim I W,Delak K,Evans J S,et al.Probing the Organic-Mineral Interface at the Molecular Level in Model Biominerals[J].Langmuir,2008,24:2680-2687.
[31]Fendler J H,Meldrum F C.The colloid-chemical approach to nanostructured materials[J].Adv.Mater.,1995,7:607-631.
[32]Lu F,Zhao X M,Zhou G D,Wang H S,et al.Control for oriented growth of large size KCl crystals by the competition between spontaneous and induced nucleation/growth on a Langmuir-Blodgett film[J].Chem.Phys.Lett.,2008,458:67-70.
[33]Taylor M G,Simkiss K,Greaves G M,Okazaki M,et al.An X-ray absorption spectroscopy study of the structure and transformation of amorphous calcium carbonate from plant cystoliths[J].Proc.Roy.Soc.London B,1993,252:75-80.
[34]Ziegler A.Ultrastructure and Electron Spectroscopic Diffraction Analysis of the Sternal Calcium Deposits of Porcellio scaber Latr.(Isopoda,Crustacea)[J].J.Struct.Bio.,1994,112:110-116.
[35]Oliver S,Kuperman A,Coombs N,Lough A,et al.Lamellar aluminophosphates with surface patterns that mimic diatom and radiolarian microskeletons[J].Nature,1995,378:47-50.
[36]Aizenberg J,Lambert L,Addadi L,Weiner S.Stabilization of amorphous calcium carbonate by specialized macromolecules in biological and synthetic precipitates[J].Adv.Mater,1996,8:222-225.
[2]Boskey A D.Mineralization of bones and teeth[M].Elements,1998(3):385-391.
[3]Marin F,Corstjens P,de Gaulejac B,de Vrind E,et al.Mucins and molluscan calcification.Molecular characterization of mucoperlin,a novel mucin-like protein from the nacreous shell layer of the fan mussel Pinna nobilis[J].J.Biol Chem.,2000,275:20667-20675.
[4]Kaplan D L.Mollusc shell structures:novel design strategies for synthetic materials[J].Curr Opin Solid State Mater.Sci.,1998,3:232-236.
[5]Wetherbee R.The diatom glasshouse[J].Science,2002,298:547-547.
[6]Chen C L,Zhang P J,Rosi N L.Hierarchical self-assembly is a fundamental principle in nature[J].J.Am.Chem.Soc.,2008,130:13555-13557.
[7]Cha J N,Stucky G D,Morse D E,Deming T J.Biomimetic synthesis of ordered silica structures mediated by block copolypeptides[J].Nature,2000,403:289-292.
[8]Raveendran P,Fu J,Wallen S L.Completely“Green”synthesis metalnanoparticles[J].J.Am.Chem.Soc.,2003,125:13940-13941.
[9]Liu B,Xie J,Lee J Y,Ting Y P,et al.Optimization of high-yield biological synthesis of single-crystalline gold nanoplates[J].J.Phys.Chem.B,2005,109:15256-15263.
[10]Raveendran P,Fu J,Wallen S L.A simple and“green”method for the synthesis of Au,Ag,and Au-Ag alloy nanoparticles[J].Green Chem,2006,8:34-38.
[11]Douglas T.A Bright Bio-Inspired Future[J].Science,2003,299:1192-1193.
[12]Yang T,Li Z,Wang L,Guo C L,et al.Synthesis,Characterization and Self-Assembly of Protein Lysozyme Monolayer-Stabilized Gold Nanoparticles[J].Langmuir,2007,23:10533-10538.
[13]Nayak N C,Shin K.Human serum albumin mediated self-assembly of gold nanoparticles into hollowspheres[J].Nanotechnology,2008,19:265603-265603.
[14]Bai X T,Zheng L Q,Li N,Dong B,et al.Synthesis and characterization of microscale gold nanoplates using Langmuir monolayers of long-chain ionic liquid[J].Cryst.Growth Des,2008,8(10):3840-3846.
[15]Sánchez-González J,Cabrerizo-Vílchez M A,Gálvez-Ruiz M J.Evaluation of the interactions between lipids andγ-globulin protein at the air-liquid interface[J].Colloids Surf.B,1999,12:123-138.
[16]Carter D C,Ho J X.Structure of serum albumin[J].Adv.Protein Chem.,1994,45:153-203.
[17]Hirayama K,Akashi S,Furuya M,Fukuhara K L.Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and frit-FAB LC/MS[J].Biochem.Biophys.Res.Commun.,1990,173:639-646.
[18]He X M,Carter D C.Atomic Structure and Chemistry of Human Serum Albumin[J].Nature,1992,358:209-215.
[19](a)Bhargava S K,Booth J M,Agrawal S,Coloe P,et al.Gold Nanoparticle Formation during Bromoaurate Reduction by Amino Acids[J].Langmuir,2005,21:5949-5956.(b)Shao Y,Jin YD,et al.Chem.Commun,2004,9:1104-1105.
[20]Xie J P,Lee J Y,Wang D I C.Protein-aided formation of triangular silver nanoprisms with enhanced SERS performance[J].J.Phys.Chem C,2007,111:10226-10232.
[21]Spadavecchia J,Prete P,Lovergine N,Tapfer L,et al.Au Nanoparticles Prepared by Physical Method on Si and Sapphire Substrates for Biosensor Applications[J].J.Phys.Chem.B,2005,109:17347-17349.
[22]Burt J L,Gutierrez-Wing C,Miki-Yoshida M,et al.Noble-Metal Nanoparticles Directly Conjugated to Globular Proteins[J].Langmuir,2004,20:11778-11783.
[23]De Oliveira D B,Laursen R A.Control of Calcite Crystal Morphology by a Peptide Designed To Bind to a Specific Surface[J].J.Am.Chem.Soc.,1997,119:10627-10631.
[24]Lu J R,Su T J,Thomas R K.Structural Conformation of Bovine Serum Albumin Layers at the Air-Water Interface Studied by Neutron Reflection[J].J.Colloid Interface Sci.,1999,213:426-437.
[25]Auduc N,Ringenbach A,Stevenson I,Jugnet Y,et al.Atomic force microscopy characterization of poly(amino acid)LangmuirBlodgett films[J].Langmuir,1993,9:3567-3573.
[26]Xue Z H,(B)Hu B,Jia X L,et al.Effect of the interaction between bovine serum albumin Langmuir monolayer and calcite on the crystallization of Ca CO3nanoparticles[J].Mater.Chem.Phys.,2009,114:47-52
[27]Kmetko J,Yu C J,Evmenenko G,Kewalramani S,et al.Evidence of Registry at the Interface during Inorganic Nucleation at an Organic Template[J].Phys.Rev.Lett.,2002,89:186102(1)-186102(4).
[28]Popescu D C,Smulders M M J,Pichon B P,et al.Template Adaptability Is Key in the Oriented Crystallization of Ca CO3[J].J.Am.Chem.Soc.,2007,129:14058-14059.
[29]Di Masi E,Kwak S Y,Pichon B P,et al.Structural adaptability in an organic template for Ca CO3 mineralization[J].Cryst.Eng.Comm.,2007,9:1192-1204.
[30]Metzler R A,Kim I W,Delak K,Evans J S,et al.Probing the Organic-Mineral Interface at the Molecular Level in Model Biominerals[J].Langmuir,2008,24:2680-2687.
[31]Fendler J H,Meldrum F C.The colloid-chemical approach to nanostructured materials[J].Adv.Mater.,1995,7:607-631.
[32]Lu F,Zhao X M,Zhou G D,Wang H S,et al.Control for oriented growth of large size KCl crystals by the competition between spontaneous and induced nucleation/growth on a Langmuir-Blodgett film[J].Chem.Phys.Lett.,2008,458:67-70.
[33]Taylor M G,Simkiss K,Greaves G M,Okazaki M,et al.An X-ray absorption spectroscopy study of the structure and transformation of amorphous calcium carbonate from plant cystoliths[J].Proc.Roy.Soc.London B,1993,252:75-80.
[34]Ziegler A.Ultrastructure and Electron Spectroscopic Diffraction Analysis of the Sternal Calcium Deposits of Porcellio scaber Latr.(Isopoda,Crustacea)[J].J.Struct.Bio.,1994,112:110-116.
[35]Oliver S,Kuperman A,Coombs N,Lough A,et al.Lamellar aluminophosphates with surface patterns that mimic diatom and radiolarian microskeletons[J].Nature,1995,378:47-50.
[36]Aizenberg J,Lambert L,Addadi L,Weiner S.Stabilization of amorphous calcium carbonate by specialized macromolecules in biological and synthetic precipitates[J].Adv.Mater,1996,8:222-225.