石墨烯对欧洲山杨组培苗不定根表观形态影响作用的研究
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摘要
研究了不同浓度石墨烯溶胶(Graphene Gel)对欧洲山杨组培苗不定根的形态、分化状况和生长状况的影响。发现适宜浓度的石墨烯能够促进植株不定根伸长、主根形成,促进不定根根数的增加,使根系健壮、颜色加深;同时植株分芽及苗高均与对照组呈显著差异,叶片颜色深绿。高浓度石墨烯处理的组培苗虽然形成明显黑褐色主根系,但须根稀少,其植株地上部分颜色浅绿略微发黄,叶片变小,正常生长发育被抑制。这表明石墨烯能够与培养基中阳离子以及植物生长激素等发生相互作用,引起植株根系微区域周围营养成分改变,导致植株发生表观形态变化。
The effects of different concentrations of grapheme gel on adventitious roots morphology, differentiation and plant growth of poplar were studied. It was found that grapheme gel of suitable concentration could promote the elongation of adventitious roots and the formation of main roots, deepen the color of roots and the number of adventitious roots also increased. Meanwhile, the plants are significantly different in budding and seedling height from the control group, and the leaf color was dark green. Grapheme gel of high concentration inhibited normal growth and development of plants, although the plantlets formed the main dark brown root system, the fibrous roots were rare, the shoot color of the plantlets was light green and yellowish, the leaves became smaller. The results showed that grapheme gel can interact with cations in the medium, resulting in a decrease in the conductivity of the medium, and cause changes in ion and growth hormone concentration around the plant roots, resulting in changes of plant morphology, differentiation and growth.
The effects of different concentrations of grapheme gel on adventitious roots morphology, differentiation and plant growth of poplar were studied. It was found that grapheme gel of suitable concentration could promote the elongation of adventitious roots and the formation of main roots, deepen the color of roots and the number of adventitious roots also increased. Meanwhile, the plants are significantly different in budding and seedling height from the control group, and the leaf color was dark green. Grapheme gel of high concentration inhibited normal growth and development of plants, although the plantlets formed the main dark brown root system, the fibrous roots were rare, the shoot color of the plantlets was light green and yellowish, the leaves became smaller. The results showed that grapheme gel can interact with cations in the medium, resulting in a decrease in the conductivity of the medium, and cause changes in ion and growth hormone concentration around the plant roots, resulting in changes of plant morphology, differentiation and growth.
引文
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[4]Arnab M, Sanghamitra M, Servin A D, et al. Carbon nanomaterials in agriculture:a critical review[J]. Frontiers in Plant Science,2016, 7:172.
[5]牛耀芳.大气CO2浓度升高对拟南芥根毛发育与养分吸收的影响及根系对养分的响应机理[D].杭州:浙江大学, 2013.
[2]Shih C J, Lin S, Sharma R, et al. Understanding the pH-dependent behavior of graphene oxide aqueous solutions:a comparative experimental and molecular dynamics simulation study[J]. Langmuir, 2012, 28(1):235-241.
[3]Dreyer D R, Park S, Bielawski C W, et al. The chemistry of graphene oxide[J]. Chem SOc Rev, 2010, 39(1):228.
[4]Arnab M, Sanghamitra M, Servin A D, et al. Carbon nanomaterials in agriculture:a critical review[J]. Frontiers in Plant Science,2016, 7:172.
[5]牛耀芳.大气CO2浓度升高对拟南芥根毛发育与养分吸收的影响及根系对养分的响应机理[D].杭州:浙江大学, 2013.