一种固相萃取自动前处理系统用于HPLC测定苯酚的研究
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摘要
研制了基于固相萃取技术的自动前处理系统,并与高效液相色谱联用,建立水中苯酚的测定方法。使用自动前处理系统,苯酚经过SPE柱富集浓缩,甲醇洗脱,经HPLC法检测,回收率在89.4%～96.4%之间。将该法用于地表水中苯酚的测定,结果令人满意。本方法实现了前处理过程自动化,操作简便,提高测定的重现性,减少操作者工作量。
This paper established a method for determination of phenol in domestic water based on SPE automatic pre-processing system coupled with HPLC analysis.With the automatic pre-processing system,the phenol was concentrated by SPE column and eluted by methanol so that the recovery rate of phenol by HPLC was between 89.4%and 96.4%.The method was applied to determination of phenol in surface water with satisfactory results.This method realizes the automation of the pre-processing,makes the operation simple,improves the reproducibility of measurement,and reduces the workload of operator.
This paper established a method for determination of phenol in domestic water based on SPE automatic pre-processing system coupled with HPLC analysis.With the automatic pre-processing system,the phenol was concentrated by SPE column and eluted by methanol so that the recovery rate of phenol by HPLC was between 89.4%and 96.4%.The method was applied to determination of phenol in surface water with satisfactory results.This method realizes the automation of the pre-processing,makes the operation simple,improves the reproducibility of measurement,and reduces the workload of operator.
引文
[1]谭晓燕,黎国兰,李松,等.水中痕量苯酚的微波萃取气相色谱分析[J].化学研究与应用,2009,21(7):1067-1067.
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[5]肖红,马莉,韩艳艳,等.液-液萃取气相色谱法测定地表水中痕量苯酚[J].化学分析计量,2016,25(4):84-86.
[2]张玲玲,顾耀秦.全自动固相萃取-高效液相色谱-荧光检测法测定生活饮用水中苯酚[J].资源节约与环保,2015,(3):80-81.
[3]候萌萌,刘小萍,曾琼梅,等.高效液相色谱法测定污水中的苯酚和间-甲酚[J].勘探开发,2015,(1):20-21.
[4]杨通在.固相萃取技术的现状与发展.勘探开发[J].环境检测与研究,2006,32(12):107-108.
[5]肖红,马莉,韩艳艳,等.液-液萃取气相色谱法测定地表水中痕量苯酚[J].化学分析计量,2016,25(4):84-86.