食用油中苯并[α]芘净化技术的优化及高效液相检测方法的建立
摘要
针对我国国标中食用油中苯并[a]芘检测方法的不足,建立高效液相色谱法-FLD检测方法,通过优化检测条件得到的最佳操作条件:检测波长:激发波长384nm,发射波长406nm;流动相为乙腈:水(梯度洗脱),在最佳实验条件下,苯并[a]芘得到良好的分离,对苯并芘标准溶液的检测分析的相对标准偏差小于5.5%,方法的检出限(3倍信噪比)和定量下限(10倍信噪比)分别为0.046μg/kg和0.155μg/kg。选用花生油、辣椒油、煎炸油为本底,通过比较中性氧化铝柱、HLB小柱、分子印迹技术、GPC凝胶色谱净化技术、QuEChERS提取净化几种不同前处理在三种食用油中苯并芘的提取效果,确定了食用油中苯并[a]芘检测的前处理技术,建立了分子印迹柱净化-效液相色谱仪联用的检测方法,优化分子印迹技术各影响因素。并考察了日常食用油和油炸油中苯并[a]芘含量情况。
引文
[1]彭小东,王欢,李红州,等.液相萃取-反相高效液相色谱法联用测定植物油中的苯并芘[J].油脂安全,2018,43(10):112-114.
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[5] AL Juhasz,R Naidu.Bioremediation of high molecular weightpolycyclic aromatic hydrocarbons:a review of the mi crobial deg radation ofbenzo[a]pyrene[J].International Biodeterioration&Biodegradation,2000,45(1):57-88.
[6]王浩,刘艳琴,杨红梅,等.高效液相色谱法测定粮食中苯并芘残留的研究[J].粮油食品科技,2007,15(1):53-54.
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[8]续颖,刘雪莹,伍蓥,等.固相萃取-液相色谱法测定食用植物油中苯并(α)芘含量[J].安徽农学通报,2017,23(6):
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[12] Puoci F,Curcio M,Cirillo G,et al.Molecularly imprinted solid phase extraction for cholesterol determination in cheese products[J].Food Chemistry,2008,106(2):836-842.
[13]朱晓玲,刘杰,范志勇,等.凝胶渗透色谱-高效液相色谱法同时测定食用油中BaP、BHA、BHT、TBHQ、DBP、DEHP[J].食品科学,2013,34(24):258-262.
[14]傅英文,花锦,王静慧,等.凝胶色谱净化-超高效液相色谱法测定食用油中苯并芘残留[J].农产品加工:学刊,2013,(10):63-65.
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[16]王珍.食用油中苯并(α)芘检测的前处理及其GC/MS分析[D].广西大学,2013.
[17]苏慧.食用油中苯并芘前处理方法的建立及其高效液相色谱分析[D].南京农业大学,2015.
[18] GB2762-2012.食品安全国家标准食品中污染物限量》[S].
[2]李进伟,王兴国,金青哲.食用油中苯并芘的来源检测和控制[J].中国油脂,2011,36(6):7-11.
[3]宋怡城.植物油中苯并芘检测及产生机理研究[J].农产品加工,2015(5):11-13.
[4]史巧巧,席俊,陆启玉.食品中苯并芘的研究进展[J].食品工业科技,2014,35(5):379-381.
[5] AL Juhasz,R Naidu.Bioremediation of high molecular weightpolycyclic aromatic hydrocarbons:a review of the mi crobial deg radation ofbenzo[a]pyrene[J].International Biodeterioration&Biodegradation,2000,45(1):57-88.
[6]王浩,刘艳琴,杨红梅,等.高效液相色谱法测定粮食中苯并芘残留的研究[J].粮油食品科技,2007,15(1):53-54.
[7] S Kira,T Katsuse,Y Nogami,et al.Measurement of Benzo(a)py rene in Sea Water and in Mussels in the Seto Inland Sea,Ja pan[J].Bulletin of Environmental Contamination&Toxicology,2000,65(5):631-637
[8]续颖,刘雪莹,伍蓥,等.固相萃取-液相色谱法测定食用植物油中苯并(α)芘含量[J].安徽农学通报,2017,23(6):
[9]袁先友,张敏.现代仪器分析与食品质量安全检测[M].成都:西南交通大学出版社,2007.163-164.
[10] Purcaro G,Moret S,Conte L S. Rapid validated methodfor the analysis of benzo(a)pyrene in vegetable oils byusing solidphase microextraction-gas chromatography-mass spectrom etry[J].JournalofChromatographyA,2007,1176(1):231-235.
[11] Caro E,.arce R M,Borrull F,et al.Application of molecularly imprinted polymers to solid-phase extraction of compounds from environmental and biological samples[J]. Trac Trends in Analytical Chemistry,2006,25(2):143-154.
[12] Puoci F,Curcio M,Cirillo G,et al.Molecularly imprinted solid phase extraction for cholesterol determination in cheese products[J].Food Chemistry,2008,106(2):836-842.
[13]朱晓玲,刘杰,范志勇,等.凝胶渗透色谱-高效液相色谱法同时测定食用油中BaP、BHA、BHT、TBHQ、DBP、DEHP[J].食品科学,2013,34(24):258-262.
[14]傅英文,花锦,王静慧,等.凝胶色谱净化-超高效液相色谱法测定食用油中苯并芘残留[J].农产品加工:学刊,2013,(10):63-65.
[15] Fromberg A,Hojgard A,Duedahl-Olesen L.Analysis of polycyclic aromatic hydrocarbons in vegetable oils combin ing gel permeation chromatography with solid-phase ex traction clean-up.[J].Food Additives&Contaminants,2007,24(7):758-767.
[16]王珍.食用油中苯并(α)芘检测的前处理及其GC/MS分析[D].广西大学,2013.
[17]苏慧.食用油中苯并芘前处理方法的建立及其高效液相色谱分析[D].南京农业大学,2015.
[18] GB2762-2012.食品安全国家标准食品中污染物限量》[S].