侧脑室注射瘦素对大鼠迷走复合体阿黑皮素原的影响
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摘要
目的研究侧脑室注射瘦素对大鼠迷走复合体阿黑皮素原的影响,探讨瘦素是否同下丘脑弓状核一样也在迷走复合体对阿黑皮素原进行调节,从而发现瘦素在迷走复合体上发挥作用的可能路径。方法 SD大鼠36只,随机分为试验组和对照组,每组18只,试验组根据注射后处死的时间(1、3、5 h)分成3个亚组,每组6只。大鼠侧脑室留置不锈钢导管1周,自由恢复及驯养,实验动物在清醒状态下于侧脑室注射瘦素3.5μg/μl,对照组注射3.5μg/μl0.9%氯化钠溶液。苏木精-伊红染色定位迷走复合体,测定阿黑皮素原在该处的表达。结果中枢注射瘦素组大鼠侧脑室注射瘦素后1、3、5 h采用免疫组化检测迷走复合体上阿黑皮素原的表达阳性细胞计数、表达阳性细胞积分光密度与生理盐水和对照组比较,差异均无统计学意义(P>0.05),中枢注射瘦素组1、3、5 h亚组组间迷走复合体上阿黑皮素原的表达阳性细胞计数、表达阳性细胞积分光密度比较,差异无统计学意义(P>0.05)。结论侧脑室注射瘦素后不能引起迷走复合体上阿黑皮素原的增多。
Objective To investigate the effects of leptin by means of lateral ventricle injection on proopiomelanocortin in dorsal vagal complexin of rats,and to explore whether the leptin can adjust proopiomelanocortin in dorsal vagal complex like hypothalamic arcuate nucleus,so as to find a possible path that leptin plays a role in vagal complex.Methods Thirty-six SD rats were randomly divided into experimental group and control group,with 18 rats in each group,moreover,the experimental group was redivided into 3 subgroups according to the death time after injection(1 h,3 h,5 h),with 6 rats in each subgroup.The stainless steel catheter was detained in lateral ventricle of rats for 1 week,then the rats were free to resume and domesticated,then the rats in experimental group were given leptin 3.5μg/μl by lateral ventricle injection,however,the rats in control group were given 0.9% sodium chloride solution 3.5μg/μl.Finally the dorsal vagal complex was located by Hematoxylin-eosin dyeing,and the expression of proopiomelanocortin was detected.Results On 1,3,5 h after giving leptin by intracerebroventricular injection,there were no significant differences in the positive expression cell count and positive expression cell integral optical density of proopiomelanocortin in dorsal vagal complex between experimental group and control group(P>0.05).Moreover there were no significant differences in the positive expression cell count and positive expression cell integral optical density of proopiomelanocortin in dorsal vagal complex among the three subgroups(P>0.05).Conclusion Injecting leptin into lateral ventricle of rats can not result in the increase of proopiomelanocortin in dorsal vagal complex.
Objective To investigate the effects of leptin by means of lateral ventricle injection on proopiomelanocortin in dorsal vagal complexin of rats,and to explore whether the leptin can adjust proopiomelanocortin in dorsal vagal complex like hypothalamic arcuate nucleus,so as to find a possible path that leptin plays a role in vagal complex.Methods Thirty-six SD rats were randomly divided into experimental group and control group,with 18 rats in each group,moreover,the experimental group was redivided into 3 subgroups according to the death time after injection(1 h,3 h,5 h),with 6 rats in each subgroup.The stainless steel catheter was detained in lateral ventricle of rats for 1 week,then the rats were free to resume and domesticated,then the rats in experimental group were given leptin 3.5μg/μl by lateral ventricle injection,however,the rats in control group were given 0.9% sodium chloride solution 3.5μg/μl.Finally the dorsal vagal complex was located by Hematoxylin-eosin dyeing,and the expression of proopiomelanocortin was detected.Results On 1,3,5 h after giving leptin by intracerebroventricular injection,there were no significant differences in the positive expression cell count and positive expression cell integral optical density of proopiomelanocortin in dorsal vagal complex between experimental group and control group(P>0.05).Moreover there were no significant differences in the positive expression cell count and positive expression cell integral optical density of proopiomelanocortin in dorsal vagal complex among the three subgroups(P>0.05).Conclusion Injecting leptin into lateral ventricle of rats can not result in the increase of proopiomelanocortin in dorsal vagal complex.
引文
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2 Park HK,Ahima RS.Physiology of leptin:energy homeostasis,neuroendocrine function and metabolism.Metabolism,2015,64:24-34.
3 希龙夫,薛荣泉,夏医君.瘦素与普通外科相关疾病.中国现代普通外科进展,2016,19:921-924.
4 Sominsky L,Ziko I,Nguyen TX,et al.Hypothalamic effects of neonatal diet:reversible and only partially leptin dependent.J Endocrinol,2017,234:41-56.
5 Dodd G,Worth A,Nunn N,et al.The thermogenic effect of leptin Is dependent on a distinct population of prolactin-releasing peptide neurons in the dorsomedial hypothalamus.Cell Metabolism,2014,20:639-649.
6 Yeo GSH,Farooqi IS,Challis BG,et al.The role of melanocortin signalling in the control of body weight:evidence from human and murine genetic models.Q J Med,2000,93:7-14.
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9 Moon HS,Dalamaga M,Kim SY,et al.Leptin’s role in lipodystrophic and nonlipodystrophic insulin-resistant and diabetic individuals.Endocr Rev,2013,34:377-412.
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12 顾海伦,刘亚莉,王维,等.瘦素对IL-1β诱导大鼠骨性关节炎软骨胞MMP-13mRNA表达的影响.东南大学学报(医学版),2015,34:870-875.
13 刘柱,范清,郑莉,等.瘦素在肿瘤坏死因子-α介导的软骨细胞死亡中的效应.临床和实验医学杂志,2016,15:642-628.
14 杨朝菊,霍丽静,王树松,等.高脂血症、糖尿病患者血清摄食抑制因子-1、脂联素水平及其相关性.中国老年医学杂志,2017,37:1374-1376.
15 朱冰,曲伸,等.瘦素调节糖代谢的机制研究进展.中国糖尿病杂志,2016,24:1040-1043.
16 丁玉静,邱丽君,徐晨,等.s LOX-1瘦素水平与代谢综合征的相关性.实用医学杂志,2017,33:813-815.
17 张志宁,赵亚恒,郑丽华,等.瘦素及瘦素受体与乳腺癌浸润、转移关系的研究.中国肿瘤外科杂志,2014,6:378-381.
18 Huo L,Grill HJ,Bjrbaek C.Divergent regulation of proopiomelanocortin neurons by leptin in the nucleus of the solitary tract and in the arcuate hypothalamic nucleus.Diabetes.2006,55:567-573.
19 Dodd GT,Decherf S,Loh K,et al.Leptin and insulin act on POMC neurons to promote the browing of white fat.Cell,2015,160:88-104.
20 Mercer J,Moar KM Hoggard N.Localization of leptin receptor(OB-R)messenger ribonucleic acid in the rodent brain.Endocrinology,1998,139:29-34.
2 Park HK,Ahima RS.Physiology of leptin:energy homeostasis,neuroendocrine function and metabolism.Metabolism,2015,64:24-34.
3 希龙夫,薛荣泉,夏医君.瘦素与普通外科相关疾病.中国现代普通外科进展,2016,19:921-924.
4 Sominsky L,Ziko I,Nguyen TX,et al.Hypothalamic effects of neonatal diet:reversible and only partially leptin dependent.J Endocrinol,2017,234:41-56.
5 Dodd G,Worth A,Nunn N,et al.The thermogenic effect of leptin Is dependent on a distinct population of prolactin-releasing peptide neurons in the dorsomedial hypothalamus.Cell Metabolism,2014,20:639-649.
6 Yeo GSH,Farooqi IS,Challis BG,et al.The role of melanocortin signalling in the control of body weight:evidence from human and murine genetic models.Q J Med,2000,93:7-14.
7 Swieten MM,Pandit R,Adan RA,et al.The neuroanatomical function of leptin in the hypothalamus.J Chem Neuroanat,2014,61/62:207-220.
8 Hausman GJ,Barb CR,Lents CA.Letin and reproductive function.Biochimie,2012,94:2075-2081.
9 Moon HS,Dalamaga M,Kim SY,et al.Leptin’s role in lipodystrophic and nonlipodystrophic insulin-resistant and diabetic individuals.Endocr Rev,2013,34:377-412.
10 Dalamaga M,Chou SH,Shields K,et al.Leptin at the intersection of neuroendocrinology and metabolism:current evidence and therapeutic perspectives.Cell Metab,2013,18:29-42.
11 Alix PM,Guebre-Egziabher F,Soulage CO.Leptin as an uremic toxin:Deleterious role of in chronic kidney disease.Biochimie,2014,105:12-21.
12 顾海伦,刘亚莉,王维,等.瘦素对IL-1β诱导大鼠骨性关节炎软骨胞MMP-13mRNA表达的影响.东南大学学报(医学版),2015,34:870-875.
13 刘柱,范清,郑莉,等.瘦素在肿瘤坏死因子-α介导的软骨细胞死亡中的效应.临床和实验医学杂志,2016,15:642-628.
14 杨朝菊,霍丽静,王树松,等.高脂血症、糖尿病患者血清摄食抑制因子-1、脂联素水平及其相关性.中国老年医学杂志,2017,37:1374-1376.
15 朱冰,曲伸,等.瘦素调节糖代谢的机制研究进展.中国糖尿病杂志,2016,24:1040-1043.
16 丁玉静,邱丽君,徐晨,等.s LOX-1瘦素水平与代谢综合征的相关性.实用医学杂志,2017,33:813-815.
17 张志宁,赵亚恒,郑丽华,等.瘦素及瘦素受体与乳腺癌浸润、转移关系的研究.中国肿瘤外科杂志,2014,6:378-381.
18 Huo L,Grill HJ,Bjrbaek C.Divergent regulation of proopiomelanocortin neurons by leptin in the nucleus of the solitary tract and in the arcuate hypothalamic nucleus.Diabetes.2006,55:567-573.
19 Dodd GT,Decherf S,Loh K,et al.Leptin and insulin act on POMC neurons to promote the browing of white fat.Cell,2015,160:88-104.
20 Mercer J,Moar KM Hoggard N.Localization of leptin receptor(OB-R)messenger ribonucleic acid in the rodent brain.Endocrinology,1998,139:29-34.