2种方法测定藻酸双酯钠(PSS)中的铵盐含量
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摘要
目的建立准确定量藻酸双酯钠(PSS)中残留铵盐的方法。方法建立了奈斯勒试剂-紫外分光光度法和离子色谱法2种铵盐含量测定方法,测定了12批PSS中的铵盐含量,并与药典定性比色的方法进行了比较分析。结果 2种方法均具有较好的准确性,回收率良好,且两种方法测得的结果具有一致性。结论这2种方法适合于PSS中铵盐的定量测定,可以作为PSS质量分析中铵盐的检查方法。
Objective To establish methods for determining the content of ammonium salt in propylene glycol alginate sodium sulfate(PSS).Methods Two methods for determining ammonium salt were established,including nessler's reagent-ultraviolet spectrophotometry and ion chromatography.The contents of ammonium salt in 12 PSS samples were determined by the two methods,which were compared with the results of qualitative colorimetric method in pharmacopeia.Results Two methods had good accuracy and recovery,and the results determined by two methods were consistent.Conclusion Two methods were suitable to the quantitative determination of ammonium salt in PSS,and could be used as analysis methods of ammonium salt in quality control of PSS.
Objective To establish methods for determining the content of ammonium salt in propylene glycol alginate sodium sulfate(PSS).Methods Two methods for determining ammonium salt were established,including nessler's reagent-ultraviolet spectrophotometry and ion chromatography.The contents of ammonium salt in 12 PSS samples were determined by the two methods,which were compared with the results of qualitative colorimetric method in pharmacopeia.Results Two methods had good accuracy and recovery,and the results determined by two methods were consistent.Conclusion Two methods were suitable to the quantitative determination of ammonium salt in PSS,and could be used as analysis methods of ammonium salt in quality control of PSS.
引文
[1]李春霞,孙杨,管华诗.海洋药物藻酸双酯钠研究进展及启示[J].生命科学,2012,24(9):1019-1025.
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[6]Wu J,Zhao X,Ren L,et al.Determination of M/G ratio of propylene glycol alginate sodium sulfate by HPLC with precolumn derivatization[J].Carbohydr Polym,2014,10:23-28.
[7]吴健,薛依婷,赵峡,等.柱前衍生液相色谱法测定藻酸双酯钠的含量[J].中国海洋药物,2013,4(32):1-7.
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[11]邵泓,郑璐侠,孙黛旎,等.离子色谱法测定谷胱甘肽等含酰胺基药物中游离铵盐的研究[J].药物分析杂志,2009,6:901-904.
[12]田青平,李文英,谢克昌.季铵盐的离子色谱分析[C].第十一届全国离子色谱学术报告会论文集,浙江杭州,2006:215.
[13]薛依婷.藻酸双酯钠(PSS)质量与生物安全关系的研究[D].青岛:中国海洋大学,2016.
[2]罗善高.藻酸双酯钠的临床应用[J].医学文选,2001,20(3):379-380.
[3]Xue Y T,Ren L,Li S,et al.Study on Quality Control of Sulfated Polysaccharide Drug,Propylene Glycol Alginate Sodium Sulfate(PSS)[J].Carbohydr Polym,2016,144:330-337.
[4]薛依婷,李春霞,管华诗.离子色谱法测定藻酸双酯钠中游离和结合硫酸根含量[J].中国海洋药物,2014,33(1):1-5.
[5]薛依婷,吴健,赵峡,等.藻酸双酯钠(PSS)分子量及其分布的测定[J].中国海洋药物,2014,33(2):1-5.
[6]Wu J,Zhao X,Ren L,et al.Determination of M/G ratio of propylene glycol alginate sodium sulfate by HPLC with precolumn derivatization[J].Carbohydr Polym,2014,10:23-28.
[7]吴健,薛依婷,赵峡,等.柱前衍生液相色谱法测定藻酸双酯钠的含量[J].中国海洋药物,2013,4(32):1-7.
[8]中国药典[S].2部:附录.北京:中国医药科技出版社,2015.
[9]万长青.氟钽酸钾中微量氮的测量[J].有色金属分析通讯,2002,4:14-16.
[10]林崇昌,司徒小玉,邓秀艳,等.酱油中铵盐含量的监测与来源分析[J].中国食品卫生杂志,2011,23(4):362-364.
[11]邵泓,郑璐侠,孙黛旎,等.离子色谱法测定谷胱甘肽等含酰胺基药物中游离铵盐的研究[J].药物分析杂志,2009,6:901-904.
[12]田青平,李文英,谢克昌.季铵盐的离子色谱分析[C].第十一届全国离子色谱学术报告会论文集,浙江杭州,2006:215.
[13]薛依婷.藻酸双酯钠(PSS)质量与生物安全关系的研究[D].青岛:中国海洋大学,2016.