铕离子(Ⅲ)荧光探针法测定阿莫西林的含量
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摘要
建立铕离子(Ⅲ)荧光探针法测定阿莫西林含量的实验方法。在激发波长为269nm和发射波长为615nm处,pH值为8.5、静置时间为5min、铕离子浓度为1.864×10~(-4)mol/L条件下,考查阿莫西林浓度对荧光强度的影响。结果表明,在1.651×10~(-5)mol/L～6.606×10~(-5)mol/L范围内,阿莫西林的浓度c与荧光强度F存在线性关系,标准曲线的线性回归方程为F=-4.450c+1268.747,线性相关系数为0.9918,平均回收率为98.93%。
Europium ion(Ⅲ) fluorescence probe method for determining the content of Amoxicillin was established. Under the condition of the excitation wavelength of 269 nm and the emission wavelength of 615 nm, the pH was 8.5, the time was 5 min, and the concentration of europium ion was 1.864×10~(-4) mol/L,the effect of amoxicillin concentration on the fluorescence intensity was investigated. The experimental results show that in the range of 1.651×10~(-5) mol/L～6.606×10~(-5) mol/L, a linear relationship between the concentration of C and the fluorescence intensity F, the linear regression equation of the standard curve was F=-4.450 c+1268.747, the correlation coefficient was 0.9918, the average recovery rate was 98.93%.
Europium ion(Ⅲ) fluorescence probe method for determining the content of Amoxicillin was established. Under the condition of the excitation wavelength of 269 nm and the emission wavelength of 615 nm, the pH was 8.5, the time was 5 min, and the concentration of europium ion was 1.864×10~(-4) mol/L,the effect of amoxicillin concentration on the fluorescence intensity was investigated. The experimental results show that in the range of 1.651×10~(-5) mol/L～6.606×10~(-5) mol/L, a linear relationship between the concentration of C and the fluorescence intensity F, the linear regression equation of the standard curve was F=-4.450 c+1268.747, the correlation coefficient was 0.9918, the average recovery rate was 98.93%.
引文
[1]孟美英.阿莫西林胶囊含量的测定[J].北方药学杂志,2015,12(2):10-11.
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[3]李文静,敖登高娃,张佩麟.以铕离子为荧光探针测定痕量依诺沙星[J].稀土,2005,26(4):47-48.
[4]李燕卿.稀土离子与抗生素类药物相互作用的荧光特性研究及应用[D].内蒙古科技大学,2015:45-47.
[5]张丽军.铽离子(Ⅲ)荧光探针法测定阿莫西林的含量[J].云南化工,2018,45(9):102-103.
[2]刘珊珊.阿莫西林及其制剂含量测定方法的研究进展[J].医学文选,2005,24(5):858.
[3]李文静,敖登高娃,张佩麟.以铕离子为荧光探针测定痕量依诺沙星[J].稀土,2005,26(4):47-48.
[4]李燕卿.稀土离子与抗生素类药物相互作用的荧光特性研究及应用[D].内蒙古科技大学,2015:45-47.
[5]张丽军.铽离子(Ⅲ)荧光探针法测定阿莫西林的含量[J].云南化工,2018,45(9):102-103.