基于线粒体COI基因鉴定蚌类的寄生钩介幼虫
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摘要
淡水蚌类发育过程中的钩介幼虫需要寄生在鱼鳃或鳍上,由于幼虫体型微小,依据其形态鉴定蚌种较困难,因此难以准确确定蚌类寄主鱼种类。通过提取寄生在黄颡鱼鳃上的已知蚌种三角帆蚌钩介幼虫DNA,扩增线粒体COI基因并测序,构建系统发育树,并计算种内、种间遗传距离。结果表明:寄生的三角帆蚌钩介幼虫和三角帆蚌聚为一支,置信度为100%,且种内分支明显小于种间分支长度。种内遗传距离为0.003,种间平均遗传距离为0.238,种间遗传距离为种内遗传距离的79.3倍。说明基于线粒体COI基因的分子标记,能够正确鉴定寄生钩介幼虫蚌种,为确定淡水蚌类寄主鱼提供了一种有效的方法。
Unionoids live as obligate parasites upon freshwater fish gills or fins during early life stage.Due to the small size,it was hard to identify by traditional motphological identification methods.In this study,the mitochondrial partial COI gene of parasitic glochidia of Hyriopsis cumingii was obtained.The phylogenetic tree was established and calculated the intra-and inter-species genetic distances.The results of clustering showed that the parasitic glochidia and target species were clustered.The genetic distance between species was 79.3times as high as the values within species.It suggested that parasitic glochidia could be used for identifying mussel species based on mitochondrial COI gene molecular markers.This technique could provide an effective method for determining the freshwater mussels host fish.
Unionoids live as obligate parasites upon freshwater fish gills or fins during early life stage.Due to the small size,it was hard to identify by traditional motphological identification methods.In this study,the mitochondrial partial COI gene of parasitic glochidia of Hyriopsis cumingii was obtained.The phylogenetic tree was established and calculated the intra-and inter-species genetic distances.The results of clustering showed that the parasitic glochidia and target species were clustered.The genetic distance between species was 79.3times as high as the values within species.It suggested that parasitic glochidia could be used for identifying mussel species based on mitochondrial COI gene molecular markers.This technique could provide an effective method for determining the freshwater mussels host fish.
引文
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[14]MATSUMOTO M.Phylogenetic Analysis of the Subclass Pteriomorphia(Bivalvia)From mtDNA COI Sequences[J].Molecular Phylogenetics&Evolution,2003,27(3):429-440.
[15]RHYMER K J M.Determination of Fish Host Use by Wild Populations of Rare Freshwater Mussels Using a Molecular Identification Key to Identify Glochidia[J].Journal of the North American Benthological Society,2009,27(1):150-160.
[2]GEIST J.Integrative Freshwater Ecology and Biodiversity Conservation[J].Ecological Indicators,2011,11(6):1507-1516.
[3]舒凤月,王海军,潘保柱,等.长江中下游湖泊贝类物种濒危状况评估[J].水生生物学报,2009,33(6):1051-1058.
[4]胡自强.中国淡水双壳类特有种的地理分布[J].动物学杂志,2005,40(6):80-83.
[5]RASHLEIGH B.Nestedness in Riverine Mussel Communities:patterns Across Sites and Fish Hosts[J].Ecography,2008,31(5):612-619.
[6]VAUGHN C C.Life History Traits and Abundance Can Predict Local Colonisation and Extinction Rates of Freshwater Mussels[J].Freshwater Biology,2012,57(5):982-992.
[7]白志毅,李家乐,潘彬斌.三角帆蚌钩介幼虫在5种寄主鱼体寄生效果的比较[J].淡水渔业,2008,38(1):3-5.
[8]华丹,徐钢春,闻海波,等.三种寄生鱼对圆背角无齿蚌寄生效果的研究[J].浙江海洋学院学报:自然科学版,2005,24(3):213-216.
[9]BOYER S L,HOWE A A,JUERGENS N W,et al.A DNA-barcoding Approach to Identifying Juvenile Freshwater Mussels(Bivalvia:Unionidae)Recovered From Naturally Infested Fishes[J].Journal of the North American Benthological Society,2011,30(1):182-194.
[10]CAMPBELL D C,JOHNSON P D,WILLIAMS J D,et al.Identification of‘extinct’Freshwater Mussel Species Using DNA Barcoding[J].Molecular Ecology Resources,2008,8(4):711-724.
[11]欧阳解秀,吴小平,欧阳珊,等.DNA条形码技术及其在蚌类保护生物学中的研究[J].中国畜牧兽医,2010,37(4):167-171.
[12]OUYANG J X,WU X P,OUYANG S,et al.Phylogenetic Analysis of Some Chinese Freshwater Unionidae Based on Mitochondrial COI Sequences[J].Journal of Conchology,2011,40(5):543-548.
[13]HUANG X C,RONG J,LIU Y,et al.The Complete Maternally and Paternally Inherited Mitochondrial Genomes of the Endangered Freshwater Mussel Solenaia carinatus(Bivalvia:Unionidae)and Implications for Unionidae Taxonomy[J].Plos One,2013,8(12):e84352-e84352.
[14]MATSUMOTO M.Phylogenetic Analysis of the Subclass Pteriomorphia(Bivalvia)From mtDNA COI Sequences[J].Molecular Phylogenetics&Evolution,2003,27(3):429-440.
[15]RHYMER K J M.Determination of Fish Host Use by Wild Populations of Rare Freshwater Mussels Using a Molecular Identification Key to Identify Glochidia[J].Journal of the North American Benthological Society,2009,27(1):150-160.