摘要
为解明高能重离子射线辐照家蚕生殖腺所产生的突变效应,以不同剂量的~(12)C~(5+)射线分别辐照家蚕幼虫不同发育时期、不同性别的生殖腺部位,以次代幼虫斑纹突变为依据鉴定辐照后代的突变率。结果显示,高能~(12)C~(5+)射线辐照家蚕幼虫生殖腺部位,可以高效诱发后代突变,对于同一发育阶段的幼虫,在1~9 Gy剂量范围内,造卵数及产卵数随幅照剂量增加而减少,而后代斑纹突变率在1~5 Gy剂量范围内随辐照剂量增加而升高;与熟蚕相比,4龄第3天幼虫辐照后诱发的后代突变率较高;家蚕幼虫辐照后诱发的突变率存在性别差异,雄性的突变率高于雌性。结果表明,对家蚕生殖腺部位进行重离子射线辐照是获得家蚕突变体的有效方法。
To investigate the mutation effect of high energy heavy ion beam radiation on Bombyx mori gonad, the gonads of different periods and sexes were irradiated with different doses of ~(12)C~(5+) ion beam. The mutation rate in sub generation can be acquired according to marking mutants in larvae. Results showed that high energy ~(12)C~(5+) ion beam irradiation could efficiently cause the marking mutation in sub generation. For larvae at the same developmental stage, the number of eggs produced and the number of eggs laid per moth decreased with the rise of irradiation dose in the range of 1 to 9 Gy, while the mutation rate in sub generation increased with the rise of irradiation dose in the range of 1 to 5 Gy. Compared with the larvae irradiated at wandering stage, higher mutation rate in sub generation was observed when irradiating on the 3 rd day of 4 th instar larvae. The mutation rate of larvae induced by irradiation was different from male to female, and was higher in male than in female. The results indicate that heavy ion beam irradiation is an effective way to obtain silkworm mutants.
引文
[1] 蒋亮,赵萍,夏庆友.家蚕抗病分子育种研究进展与展望[J].蚕业科学,2014,40(4):571-575
[2] 中国农业科学院蚕业研究所.家蚕遗传育种学[M].北京:科学出版社,1981:271-273
[3] 陈震古.激光诱变家蚕突变及其遗传育种的研究[J].激光生物学,1994,3(2):433-440
[4] 代方银,鲁成,王霞,等.家蚕60Co-γ射线诱发油蚕的遗传学研究[J].西南农业大学学报,2002,24(2):173-174
[5] KRAFT G.Radiobiological effects of very heavy Ions:inactivation,induction of chromosome aberrations and strand breaks[J].Nucl Sci Appl,1987,3:1-28
[6] BLAKELY E A.Cell inactivation by heavy charged particles[J].Radiat Environ Biophys,1992,31(3):181-196
[7] GOODHEAD D T.Initial events in the cellular effects of ionizing radiation:clustered damage in DNA[J].Int J Radiat Biol,1994,65(1):7-17
[8] RYDBERG B,LOBRICH M,COOPER P K.DNA double-strand breaks induced by high-energy neon and iron ions in human fibroblasts.Ⅰ.Pulsed-field gel electrophoresis method[J].Radiat Res,1994,139(2):133-141
[9] TANAKA A,SHIKAZONO N,YOKOTA Y,et al.Effects of heavy ions on the germination and survival of Arabidopsis thaliana[J].Int J Radiat Biol,1997,72(1):121-127
[10] HASE Y,SHIMONO K,INOUE M,et al.Biological effects of ion beams inNicotiana tabacum L.[J].Radiat Environ Biophys,1999,38(2):111-115
[11] TU Z L,KOBAYASHI Y,KIGUCHI K,et al.Applicability of radiosurgery with heavy ion beams to inactivate specific organs in living organisms[J].Radiat Environ Biophys,2002,41(3):231-234
[12] CHEN J,WEI J S,YAN S,et al.Initial work on applying ion implantation technology in silkworm Bombyx mori L.morphology and genetics studies[J].Dev Reprod Biol,1998,7(2):35-41
[13] 庄大桓,桂仲争,姜玉兰,等.利用生物卫星搭载家蚕试验初报[J].蚕业科学,1995,21(3):135-138
[14] MURAKAMI A,MIKI M.Age-dependent changes of radiosensitivity in embryo of Bombyx mori[J].J Radiat Res,1972,13(4):183-192
[15] YAMAGUCHI H.Radiation biology[M].Tokyo:Japan Shokabo Press,1995:124-135
[16] 刘海涛,李兵,王国宝,等.辛硫磷对家蚕雌雄个体的毒力比较[J].江苏农业科学,2010(6):390-392
[17] 黄桂辉,孙运朋,何静,等.家蚕雌雄蛾冷藏对产卵性能的影响[J].现代农业科技,2013(6):259-262
[18] 阮成龙,米智,杜文华,等.氟化物对不同抗性家蚕品种的GST和AChE酶活性影响及雌雄个体间的酶活性差异[J].蚕业科学,2012,38(5):845-849
[19] 林英,陈冬妹,代方银,等.家蚕化学诱变剂及诱导突变体的筛选[J].昆虫学报,2007,50(5):435-440
[20] 赵丽华,李文建,金卫明,等.碳离子射线辐射对家蚕经济性状的影响[J].蚕桑通报,2015,46(1):28-31