Graves病初发患者血清白细胞介素-4测定分析
|
摘要
目的探讨Graves病初发患者与健康体检人群血清IL-4浓度差异有无统计学意义。方法收集56名GD初发患者血清并测定其游离三碘甲状腺原氨酸(FT_3)、游离甲状腺素(FT_4)、促甲状腺激素(TSH)、促甲状腺激素受体抗体(AntiTSHR)、甲状腺球蛋白抗体(Anti-TG)、甲状腺过氧化物酶抗体(Anti-TPO)和IL-4浓度。GD初发患者经临床表现、体格检查、血清甲状腺激素测定结合甲状腺彩超结果确诊。根据患者血清FT_4增高的程度将患者组分为轻度增高组和重度增高组。收集与患者年龄均值和性别比例相匹配的50名健康体检人员血清并测定其IL-4浓度。采用SPSS 13.0统计软件进行数据的统计学处理,各计量资料以x±s表示,两样本均数的比较采用t检验,以P <0. 05为差异有统计学意义。对GD初发患者血清IL-4与Anti-TSHR、Anti-TG和Anti-TPO进行pearson相关性分析。结果经t检验分析,GD初发患者与健康体检者血清IL-4浓度相比P=0.03(<0.05),差异有统计学意义,GD初发患者体内血清IL-4浓度明显高于健康体检者。FT_4轻度增高组和重度增高组间IL-4浓度相比较P=0. 57(>0. 05),差异无统计学意义。经Pearson相关性分析,GD初发患者血清IL-4与Anti-TSHR、Anti-TG和Anti-TPO的P值分别为0.50、0.43、0.90,均无线性相关关系。结论 GD初发患者血清IL-4浓度明显高于健康体检者,FT_4轻度增高组和重度增高组间IL-4浓度差异无统计学意义,IL-4与Anti-TSHR、Anti-TG和Anti-TPO均无线性相关关系。
Objective This study was purposed to investigate whether the patients' serum interleukin-4(IL-4)concentration of the incipient Graves' disease(GD)had significant difference from healthy physical examination control subjects. Methods We collected 56 incipient GD patients' serum and analyzed the IL-4,FT_3,FT_4,TSH,Anti-TSHR, Anti-TG and Anti-TPO concentrations. The patients were diagnosed by physical examination,thyroid related hormones testing and thyroid ultrasonography. 50 healthy physical examination peoples' serum were collected and IL-4 concentrations were tested as well. We divided the patients into two groups,including the mild group and the severe group according to the FT_4 values. The control subjects were matched by age and gender with the patient group. We statistical analyzed the IL-4 concentration data between patients and healthy control people,also between the mild group and the severe group with t test by SPSS 13. 0 software. Linear correlation was applied to analyze the relationship between IL-4 concentration and Anti-TSHR,Anti-TG,AntiTPO concentrations of the GD patients. Results We found significantly higher concentrations of IL-4 of GD patients compared to the healthy subjects,(P <0.05). Moreover,there was no significant difference between the mild group and the severe group(P >0.05). No linear correlation was found among IL-4,Anti-TSHR,Anti-TG and Anti-TPO concentrations, and the P values were 0. 50,0.43 and 0. 90, respectively. Conclusion Our research demonstrated that the IL-4 concentrations of the incipient Graves ' disease patients ' serum are significantly higher than healthy physical examination control subjects. No significant difference was found between the GD mild group and the severe group in IL-4 concentration. IL-4 has no significant linear correlation with Anti-TSHR,Anti-TG and Anti-TPO concentrations.
Objective This study was purposed to investigate whether the patients' serum interleukin-4(IL-4)concentration of the incipient Graves' disease(GD)had significant difference from healthy physical examination control subjects. Methods We collected 56 incipient GD patients' serum and analyzed the IL-4,FT_3,FT_4,TSH,Anti-TSHR, Anti-TG and Anti-TPO concentrations. The patients were diagnosed by physical examination,thyroid related hormones testing and thyroid ultrasonography. 50 healthy physical examination peoples' serum were collected and IL-4 concentrations were tested as well. We divided the patients into two groups,including the mild group and the severe group according to the FT_4 values. The control subjects were matched by age and gender with the patient group. We statistical analyzed the IL-4 concentration data between patients and healthy control people,also between the mild group and the severe group with t test by SPSS 13. 0 software. Linear correlation was applied to analyze the relationship between IL-4 concentration and Anti-TSHR,Anti-TG,AntiTPO concentrations of the GD patients. Results We found significantly higher concentrations of IL-4 of GD patients compared to the healthy subjects,(P <0.05). Moreover,there was no significant difference between the mild group and the severe group(P >0.05). No linear correlation was found among IL-4,Anti-TSHR,Anti-TG and Anti-TPO concentrations, and the P values were 0. 50,0.43 and 0. 90, respectively. Conclusion Our research demonstrated that the IL-4 concentrations of the incipient Graves ' disease patients ' serum are significantly higher than healthy physical examination control subjects. No significant difference was found between the GD mild group and the severe group in IL-4 concentration. IL-4 has no significant linear correlation with Anti-TSHR,Anti-TG and Anti-TPO concentrations.
引文
[1]LI Q, WANG B, MU K, et al. The pathogenesis of thyroid autoimmune diseases:New T lymphocytes-Cytokines circuits beyond the Th1-Th2paradigm[J]. J Cell Physiol.,2019,234(3):2204-2216.
[2]李霞,栾树荣,耿秀英,血清白细胞介素-6在Graves病中的临床意义[J].中国综合临床,2010,26(6):603-605.
[3]KABATA H,MORO K, KOYASU S. The group 2 innate lymphoid cell(ILC2)regulatory network and its underlying mechanisms[J].Immunological reviews,2018.286(1):p. 37-52.
[4]ESHAGHKHANI Y, SANATI M H, NAKHJAVANI M, et al,Disturbed Thl and Th2 balance in patients with Graves'disease[J].Minerva Endocrinol,2016.41(1):28-36.
[5]谢建平,吴成秀,严宗逊,等.Graves病治疗前后细胞因子水平的变化[J].中华核医学杂志,2004,24(4):247-249.
[6]YABIKU K, HAYASHI M, KOMIYA I, et al, Polymorphisms of interleukin(IL)-4 receptor alpha and signal transducer and activator of transcription-6(Stat6)are associated with increased IL-4RalphaStat6signalling in lymphocytes and elevated serum IgE in patients with Graves'disease[J]. Clinical and experimental immunology,2007,148(3):425-431.
[7]郭成,张海清,Graves病免疫相关机制的研究进展[J].细胞与分子免疫学杂志,2015.31(3):416-418.
[8]ZHENG L,YE P,LIU C. The role of the IL-23/IL-17 axis in the pathogenesis of Graves'disease[J]. Endocr J, 2013. 60(5):591-597.
[2]李霞,栾树荣,耿秀英,血清白细胞介素-6在Graves病中的临床意义[J].中国综合临床,2010,26(6):603-605.
[3]KABATA H,MORO K, KOYASU S. The group 2 innate lymphoid cell(ILC2)regulatory network and its underlying mechanisms[J].Immunological reviews,2018.286(1):p. 37-52.
[4]ESHAGHKHANI Y, SANATI M H, NAKHJAVANI M, et al,Disturbed Thl and Th2 balance in patients with Graves'disease[J].Minerva Endocrinol,2016.41(1):28-36.
[5]谢建平,吴成秀,严宗逊,等.Graves病治疗前后细胞因子水平的变化[J].中华核医学杂志,2004,24(4):247-249.
[6]YABIKU K, HAYASHI M, KOMIYA I, et al, Polymorphisms of interleukin(IL)-4 receptor alpha and signal transducer and activator of transcription-6(Stat6)are associated with increased IL-4RalphaStat6signalling in lymphocytes and elevated serum IgE in patients with Graves'disease[J]. Clinical and experimental immunology,2007,148(3):425-431.
[7]郭成,张海清,Graves病免疫相关机制的研究进展[J].细胞与分子免疫学杂志,2015.31(3):416-418.
[8]ZHENG L,YE P,LIU C. The role of the IL-23/IL-17 axis in the pathogenesis of Graves'disease[J]. Endocr J, 2013. 60(5):591-597.