黄茶对草鱼幼鱼几种代谢酶活性的影响
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摘要
将黄茶以0%、0.075%、0.15%、0.30%、0.60%、1.20%与2.40%的剂量添加于草鱼(Ctenopharyngodon idellus)饲料中,研究其对草鱼几种重要代谢酶活性的影响。结果显示,黄茶降低了γ-谷氨酰转肽酶、谷丙转氨酶以及乳酸脱氢酶的活性,添加量越高则酶活性越低,其中多数试验组与对照组差异达到显著水平(P<0.05);谷草转氨酶活性在0.15%～0.60%试验组与对照组无显著差异,其余各试验组均显著低于对照组,碱性磷酸酶除0.60%组显著高于对照组外(P<0.05),其余各试验组基本与对照组无显著差异(P>0.05);脂肪酸合成酶、脂蛋白脂酶活性、肝脂酶活性以及总脂酶活性均比对照组显著下降(P<0.05)。结果表明,黄茶能显著影响代谢酶活性,特别对脂肪代谢酶活性的抑制作用更强。
In order to investigate the effects of Chinese yellow tea on several important activities of proteins and fat metabolism enzymes in grass carp(Ctenopharyngodon idellus),6 kinds of diets of Chinese yellow tea was adding into the diets in the feed of grass carp at 0%,0.075%,0.15%,0.30%,0.60%,1.20%and 2.40% supplementation levels)were tested.The results showed that for Chinese yellow tea groups,the activities ofγ-Glutamyl transpeptidase,alanine aminotransferase and lactate dehydrogenase decreased compared with CP(group 0%),and the higher the addition was,the lower the enzymes activity was,and the activities of enzymes in most of Chinese yellow tea groups decreased significantly compared with CP(P<0.05).There was no significant difference in aminotransferase activity between some Chinese yellow tea groups(groups of 0.15%～0.60 %)and CP(P>0.05),and other test groups were significant lower than CP(P<0.05).The activity of alkaline phosphatase was significantly higher than CP except group 0.60%(P<0.05),and the other test groups were not significant difference from CP(P>0.05).The activities of fatty acid synthase,lipoprotein lipase,hepatic lipase and total lipase decreased significantly compared with CP(P <0.05).The results indicated that Chinese yellow tea could significantly affect the activities of metabolic enzymes and inhibit the activities of fat metabolism enzymes.
In order to investigate the effects of Chinese yellow tea on several important activities of proteins and fat metabolism enzymes in grass carp(Ctenopharyngodon idellus),6 kinds of diets of Chinese yellow tea was adding into the diets in the feed of grass carp at 0%,0.075%,0.15%,0.30%,0.60%,1.20%and 2.40% supplementation levels)were tested.The results showed that for Chinese yellow tea groups,the activities ofγ-Glutamyl transpeptidase,alanine aminotransferase and lactate dehydrogenase decreased compared with CP(group 0%),and the higher the addition was,the lower the enzymes activity was,and the activities of enzymes in most of Chinese yellow tea groups decreased significantly compared with CP(P<0.05).There was no significant difference in aminotransferase activity between some Chinese yellow tea groups(groups of 0.15%～0.60 %)and CP(P>0.05),and other test groups were significant lower than CP(P<0.05).The activity of alkaline phosphatase was significantly higher than CP except group 0.60%(P<0.05),and the other test groups were not significant difference from CP(P>0.05).The activities of fatty acid synthase,lipoprotein lipase,hepatic lipase and total lipase decreased significantly compared with CP(P <0.05).The results indicated that Chinese yellow tea could significantly affect the activities of metabolic enzymes and inhibit the activities of fat metabolism enzymes.
引文
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[3]Ishihara N,Araki T,Tamaru Y,et al.Influence of green tea polyphenols on feed performance,growth performance,and fish body composition in yellowtail(Seriola quinqueradiata)[J].Jpn J Food Chem,2002,9:7~14.
[4]Caberra C,Artacho R,Gimenez R.Beneficial effects of Green tea-A Review[J].Journal of the American College of Nutrition,2006,25:79~99.
[5]马倩倩,宛晓春,杨严鸥,等.红茶对草鱼几种代谢酶活性的影响[J].长江大学学报(自科版),2018,15(14):21~23.
[6]崔燕芒.黄山毛峰和祁门红茶中多酚类成分分析及其抗肝损伤作用研究[D].西安:第四军医大学,2014.
[7]吴艳艳.黄茶、白茶的化学成分研究[D].大连:辽宁师范大学,2013.
[8]边世平.茶叶的化学成分及其保健作用[J].青海大学学报(自然科学版),2004,(8):64~67.
[9]桑巴一.碎茶对草鱼生产性能及肉中脂类的影响[D].合肥:安徽农业大学,2015.
[10]商书霞,军魁,孙秋杰,等.血清总胆红素和γ-谷氨酰转肽酶与2型糖尿病患者低度白蛋白尿的相关性研究[J].现代中西医结合杂志,2018,27(23):2521~2524,2528.
[11]马骏,李勇,张静,等.3种非营养性抗氧化剂在水产动物中的研究进展[J].水产科学,2018,37(3):414~420.
[12]Burrells C,Williams P D,Southgate P J.Immunological,physiological and pathological responses of rainbow trout(Oncorhynchus mykiss)to increasing dietary concentrating of soybean proteins[J].Veterinary Immunology and Immunopathology,1999,72:277~288.
[13]王桂芹,李子平,牛小天,等.饲料能量和维生素B6对乌鳢生长和蛋白质代谢酶活性的影响[J].中山大学学报(自然科学版),2011,50(4):96~99.
[14]王茜,姜美玲,武玉珑,等.苯酚对多刺裸腹溞糖及蛋白质代谢酶的影响[J].动物学杂志,2010,45(6):78~84.
[15]李二超,陈立侨,曾嶒,等.盐度对凡纳滨对虾体组织蛋白质积累、氨基酸组成和转氨酶活性的影响[J].水生生物学报,2009,33(3):532~538.
[16]陈文,陈代文,黄艳群.脂蛋白脂酶(LPL)生理功能及特异表达[J].中国畜牧兽医,2004,23(4):29~30.
[17]张晓刚.肝脂酶与脂蛋白代谢[J].国外医学·临床生物化学与检验学分册,2002,(1):48~49.
[18]李强,曹立梅,陈旭.肝脂酶基因多态性与甘油三酯的相关性研究[J].山西医药杂志,2017,46(21):2563~2566.