蛋氨酸对瓦氏黄颡鱼幼鱼肝脏及血浆中谷草转氨酶和谷丙转氨酶活力的影响
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摘要
本文旨在研究人工饲料中蛋氨酸对瓦氏黄颡鱼幼鱼肝脏及血浆中谷草转氨酶和谷丙转氨酶活力的影响。在基础饲料中分别添加0.0、0.3、0.6、0.9、1.2和1.5g/100g的晶体DL-Met,以L-Glu作为等氮替代物,配制6组等氮等脂饲料(Met的实测值为0.40、0.73、1.04、1.33、1.70和1.98g/100g饲料),在室内循环水系统中进行为期70d的生长实验。实验表明,随着饲料中蛋氨酸含量的增加,肝脏谷丙转氨酶(GPT)活力显著升高,并在蛋氨酸含量为1.70g/100g饲料时,活力达最大值(P<0.05);肝脏谷草转氨酶(GOT)活力与GPT活力有相似的变化趋势,但各处理组间无显著性差异(P>0.05)。随着饲料中蛋氨酸含量的增加,血浆中的GPT活力呈现先升高后平稳的变化趋势,且拐点出现在蛋氨酸含量为1.04g/100g(P<0.05)饲料组,而血浆中GOT活力呈现先升高后降低的变化趋势,且最大值出现在蛋氨酸含量为0.73g/100g(P<0.05)饲料组。研究表明,当饲料中蛋氨酸含量不足或过量时,都会影响瓦氏黄颡鱼幼鱼转氨酶的活力,从而在一定程度上影响鱼体的氨基酸代谢。
A 10-week feeding trialina recirculation rearing system was tried in order to determine the influence of dietary methionine on the activity of glutamic-pyruvic and glutamic oxalacetic transaminasesin the liver and plasma of juvenile darkbarbel catfish(Pelteobagrus vachelli).Six nitrogen and lipid contents identicaldiets were formulated by supplementing 0.0%,0.3%,0.6%,0.9%,1.2%and 1.5%of methionine,making the final dietary methionine content to be 0.40%,0.73%,1.04%,1.33%,1.70% and1.98%,respectively.The results showed that when dietary methionine increased,the activity of glutamic-pyruvic transaminase(GPT)in liver increased significantly(P<0.05),reaching the highest when the content of diet methionine was 1.70%,while the activity of glutamicoxalacetictransaminase(GOT)in liver increased in the same trend as that of GPT although no significantdifferencewas found among diets.As dietary methionine increased,the activities of plasma GPTincreased first and then turned to be stable when the content of methionine was 1.04%(P<0.05).Similarly,the activity of plasma GOTfirst increased,reaching the highest when the content of methionine was 0.73%and then decreased(P<0.05).In conclusion,the activity of two transaminases wassignificantly influenced by dietary methionine.
A 10-week feeding trialina recirculation rearing system was tried in order to determine the influence of dietary methionine on the activity of glutamic-pyruvic and glutamic oxalacetic transaminasesin the liver and plasma of juvenile darkbarbel catfish(Pelteobagrus vachelli).Six nitrogen and lipid contents identicaldiets were formulated by supplementing 0.0%,0.3%,0.6%,0.9%,1.2%and 1.5%of methionine,making the final dietary methionine content to be 0.40%,0.73%,1.04%,1.33%,1.70% and1.98%,respectively.The results showed that when dietary methionine increased,the activity of glutamic-pyruvic transaminase(GPT)in liver increased significantly(P<0.05),reaching the highest when the content of diet methionine was 1.70%,while the activity of glutamicoxalacetictransaminase(GOT)in liver increased in the same trend as that of GPT although no significantdifferencewas found among diets.As dietary methionine increased,the activities of plasma GPTincreased first and then turned to be stable when the content of methionine was 1.04%(P<0.05).Similarly,the activity of plasma GOTfirst increased,reaching the highest when the content of methionine was 0.73%and then decreased(P<0.05).In conclusion,the activity of two transaminases wassignificantly influenced by dietary methionine.
引文
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[13]万松良,黄永涛,刘敏,等.瓦氏黄颡鱼的含肉率及营养成分分析[J].水利渔业,2008,28(3):59-61.
[14]封福鲜,艾庆辉,徐玮,等.精氨酸和赖氨酸对瓦氏黄颡鱼幼鱼生长和非特异性免疫力的影响[J].水产学报,2011,123(31):420.
[15]张世亮.饲料中糖结构、糖水平及糖脂比对瓦氏黄颡鱼幼鱼生长及糖代谢的影响[D].青岛:中国海洋大学,2011.
[16]郑珂珂,朱晓鸣,韩冬,等.饲料脂肪水平对瓦氏黄颡鱼生长及脂蛋白脂酶基因表达的影响[J].水生生物学报,2010,34(4):815-821.
[17]Wang X L,Ai Q H,Mai K S,et al.Effects of dietary methionine on survival,growth performance,metabolicand immune enzymes,molecular cloning and expression of methionine metabolism genes of juvenile darkbarbel catfish(Pelteobagrusvachelli)at a constant dietary cystine level[J].Aquaculture,(unpublished).
[18]Broer S.Amino acid transport across mammalian intestinal and renal epithelia[J].Physiological Reviews,2008,88(1):249-286.
[19]Cheng Z Y,Ai Q H,Mai K S,et al.Effects of dietary canola meal on growth performance,digestion and metabolism of Japanese seabass,Lateolabrax japonicas[J].Aquaculture,2010,305(1):102-108.
[20]郑永华,蒲富永.贡对鲤鲫鱼组织转氨酶活性的影响[J].西南农业大学学报,1997,19(1):41-45.
[21]Lin L,Zeng X L,Zhang J.Effect of profenofos poisoning on liver lipid peroxidation and liver function in rabbits[J].Chinese Journal of Clinic Rehabilitation,2004,8(21):4380-4381.
[2]Tulli F,Messina M,Calligaris M,et al.Response of European sea bass(Dicentrarchuslabrax)to graded levels of methionine(total sulphur amino acids)in soyaprotein-based semi-purified diets[J].Brit J Nutr,2010,104:664-673.
[3]Goff J B,Gatlin D M.Evaluation of different sulfur amino acid compounds in the dietof red drum,Sciaenopsocellatus,and sparing value of cysteine for methionine[J].Aquaculture,2004,241:465-477.
[4]Zhou Q C,Wu Z H,Tan B P,et al.Optimal dietary methionine requirement for juvenile cobia(Rachycentroncanadum)[J].Aquaculture,2006,258:551-557.
[5]Mai K S,Wan J L,Ai Q H,et al.Dietary methionine requirement of juvenile yellow croaker Pseudosciaenacrocea R[J].Aquaculture,2006,251:564-572.
[6]Twibell R G,Wilson K A,Brown P B.Dietary sulfur amino acid requirement of juvenileyellow perch fed the maximum cystine replacement value for methionine[J].J Nutr,2000,130:612-616.
[7]Ahmed I,Khan M A,Jafri A K.Dietary methionine requirement of fingerling Indian major carp,Labeorohita(Hamilton)[J].Aquaculture International,2003,11:449-462.
[8]王桂芹,李子平,牛小天,等.饲料能量和维生素B_6对乌鳢生长和蛋白质代谢酶活性的影响[J].中山大学学报(自然科学版),2011,50(4):96-99.
[9]Yan Q,Xie S,Zhu X,et al.Dietary methionine requirement for juvenile rockfish,Sebastesschlegeli[J].Aquac Nutr,2007,13:163-169.
[10]杨英豪.大豆浓缩蛋白对瓦氏黄颡鱼幼鱼摄食、生长、消化和蛋白质代谢的影响[D].青岛:中国海洋大学,2011.
[11]Feng L,Xiao W W,Liu Y,et al.Methionine hydroxyl analogue prevents oxidative damage and improves antioxidant status of intestine and hepatopancreas for juvenile Jian carp(Cyprinuscarpio var.Jian)[J].Aquacult Nutr,2011,17:595-604.
[12]Niu J,Du Q,Lin H Z,et al.Quantitative dietary methionine requirement of juvenile golden pompanoTrachinotusovatus at a constant dietary cystine level[J].Aquacult Nutr,2013,19:677-686.
[13]万松良,黄永涛,刘敏,等.瓦氏黄颡鱼的含肉率及营养成分分析[J].水利渔业,2008,28(3):59-61.
[14]封福鲜,艾庆辉,徐玮,等.精氨酸和赖氨酸对瓦氏黄颡鱼幼鱼生长和非特异性免疫力的影响[J].水产学报,2011,123(31):420.
[15]张世亮.饲料中糖结构、糖水平及糖脂比对瓦氏黄颡鱼幼鱼生长及糖代谢的影响[D].青岛:中国海洋大学,2011.
[16]郑珂珂,朱晓鸣,韩冬,等.饲料脂肪水平对瓦氏黄颡鱼生长及脂蛋白脂酶基因表达的影响[J].水生生物学报,2010,34(4):815-821.
[17]Wang X L,Ai Q H,Mai K S,et al.Effects of dietary methionine on survival,growth performance,metabolicand immune enzymes,molecular cloning and expression of methionine metabolism genes of juvenile darkbarbel catfish(Pelteobagrusvachelli)at a constant dietary cystine level[J].Aquaculture,(unpublished).
[18]Broer S.Amino acid transport across mammalian intestinal and renal epithelia[J].Physiological Reviews,2008,88(1):249-286.
[19]Cheng Z Y,Ai Q H,Mai K S,et al.Effects of dietary canola meal on growth performance,digestion and metabolism of Japanese seabass,Lateolabrax japonicas[J].Aquaculture,2010,305(1):102-108.
[20]郑永华,蒲富永.贡对鲤鲫鱼组织转氨酶活性的影响[J].西南农业大学学报,1997,19(1):41-45.
[21]Lin L,Zeng X L,Zhang J.Effect of profenofos poisoning on liver lipid peroxidation and liver function in rabbits[J].Chinese Journal of Clinic Rehabilitation,2004,8(21):4380-4381.