Hoxc13基因在苏博美利奴羊胎羔生长发育期不同组织中的表达分析
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摘要
【目的】分析苏博美利奴羊不同组织中Hoxc13基因mRNA的表达量差异,为分子标记辅助育种以及,研究细毛羊主要经济性状,奠定理论基础。【方法】使用RT-PCR技术检测Hoxc13基因在苏博美利奴羊65和135 d皮肤、肌肉和不同内脏组织(心脏、脾脏、肝脏、肺脏、肾脏)中mRNA的表达量,并采用相对定量法对其表达量进行统计分析。【结果】在苏博美利奴羊皮肤、肌肉和不同内脏组织中均有Hoxc13基因的表达,并且在皮肤中的表达量显著高于其它组织(P<0.05),毛囊的形成和毛发生长与Hoxc13基因密切相关。【结论】建立了胎龄分别在65和135 d的苏博美利奴羊,其不同组织中适于定量检测的Hoxc13基因表达量的RT-PCR方法,从分子水平探讨了苏博美利奴羊的组织器官与Hoxc13基因表达之间的内在联系。
【Objective】 The main purpose of this study is to lay a theoretical foundation for further development of molecular marker-assisted breeding of important economic traits of fine wool sheep by analyzing the expression difference of Hoxc13 gene mRNA in different tissues of Subo Merino sheep. 【Methods】In this study, RT-PCR was used to detect the mRNA expression of Hoxc13 gene in skin, muscle and different visceral tissues(heart, spleen, liver, lung, kidney) in Subo Merino for 65 days and 135 days, and the relative quantitative method was used to conduct the statistical analysis.【Results】The results showed that the Hoxc13 gene was expressed in skin and different tissues of Subo Merino sheep, and the expression level in the skin was significantly higher than that of other tissues(P<0.05).【Conclusion】This experiment establishes a RT-PCR method of the expression of Hoxc13 for detection of the gestational ages of 65 and 135 days of Subo Merino sheep in its different tissues in order to investigate the intrinsic link between the different tissues and the expression of Hoxc13 of Subo Merino sheep at the molecular level.
【Objective】 The main purpose of this study is to lay a theoretical foundation for further development of molecular marker-assisted breeding of important economic traits of fine wool sheep by analyzing the expression difference of Hoxc13 gene mRNA in different tissues of Subo Merino sheep. 【Methods】In this study, RT-PCR was used to detect the mRNA expression of Hoxc13 gene in skin, muscle and different visceral tissues(heart, spleen, liver, lung, kidney) in Subo Merino for 65 days and 135 days, and the relative quantitative method was used to conduct the statistical analysis.【Results】The results showed that the Hoxc13 gene was expressed in skin and different tissues of Subo Merino sheep, and the expression level in the skin was significantly higher than that of other tissues(P<0.05).【Conclusion】This experiment establishes a RT-PCR method of the expression of Hoxc13 for detection of the gestational ages of 65 and 135 days of Subo Merino sheep in its different tissues in order to investigate the intrinsic link between the different tissues and the expression of Hoxc13 of Subo Merino sheep at the molecular level.
引文
[1] 徐丽.超细型细毛羊新品种--苏博美利奴羊[J].农村百事通,2015,(12):43-44,73.XU Li.(2015).A new type of superfine fine wool sheep - Subo Merino [J].Rural Know-How,(12):43-44,73.(in Chinese)
[2] 柳楠,田可川,石国庆,等.苏博美利奴羊核心群级进杂交不同世代对产毛性状影响的研究[J].中国畜牧杂志,2015,51(15):6-10.LIU Nan,TIAN ke-chuan,SHI Guo-qing,et al.(2015).Study on the Influence of Different Generations of Subo Merino Sheep Core Group Progressive Hybrids on Hair Growth Traits [J].Chinese Journal of Animal Science,51(15):6-10.(in Chinese)
[3] 田月珍,黄锡霞,柏妍,等.与毛囊生长发育及羊毛、羊绒品质相关基因的研究进展[J].中国草食动物科学,2013,33(3):55-58.TIAN Yue-zhen,HUANG Xi-xia,BAI Yan,et al.(2013).Advances in research on genes related to hair follicle growth and development,wool and cashmere quality [J].Chinese Herbivore Science,33(3):55-58.(in Chinese)
[4] 王宁,荣恩光,闫晓红.毛囊发育与毛发生产研究进展[J].东北农业大学学报,2012,(9):6-12.WANG Ning,RONG En-guang,YAN Xiao-hong,et al.(2012).Research progress on hair follicle development and hair production [J].Journal of Northeast Agricultural University,(9):6-12.(in Chinese)
[5] Rogers,G.E.(2006).Biology of the wool follicle:an excursion into a unique tissue interaction system waiting to be re-discovered.Experimental dermatology,15(12):931-949.
[6] Galbraith,H.(2010).Fundamental hair follicle biology and fine fiber production in animals.Animal,4(9):1,490-1,509.
[7] 吴江鸿.Hoxc13基因在绒山羊皮肤中的表达规律及体外功能分析[D].呼和浩特:内蒙古农业大学,2011.WU Jiang-hong.(2011).Expression of Hoxc13 Gene in Cashmere Goat Skin and Analysis of Its Function in Vitro [D].Master Dissertation.Inner Mongolia Agricultural University,Hohhot.(in Chinese)
[8] 夏辉,李龙江.Hox基因与肿瘤发生发展的研究进展[J].国际口腔医学杂志,2007,34(6):412-414.XIA Hui,LI Long-jiang.(2007).Advances in research on Hox gene and tumorigenesis [J].International Journal of Stomatology,34(6):412-414.(in Chinese)
[9] 金淑清,浦予飞,裘莹.Hox基因的研究进展[J].癌症进展,2011,9(2):154-158.JIN Shu-qing,FU Yu-fei,QIU Ying.(2011).Research progress of Hox gene [J].Cancer Progression,9(2):154-158.(in Chinese)
[10] Yang,M.,Li,Q.F.,& Zhang,F.(2010).Hox genes in the skin.Chinese medical journal,123(18):2,607-2,612.
[11] Hui-Feng,S.,Jia-Ke,C.,& Min-Liang,C..(2005).Differential display of homeobox gene expressions in the normal,wounded human fetal and adult skins by DNA microarray.Chinese Journal of Plastic Surgery & Burns,21(5):372-374.
[12] 白玉妍,范衡宇,杨增明.同源盒基因(Hox)与哺乳动物生殖[J].生物化学与生物物理进展,2001,28(3):329-332.BAI Yu-yan,FAN Heng-yu,YANG Zeng-ming.(2001).Homeobox gene (Hox) and mammalian reproduction [J].Progress in Biochemistry and Biophysics,28(3):329-332.(in Chinese)
[13] 张燕军,李金泉,尹俊,等.Homeobox基因对毛囊生长发育作用机制的研究进展[J].中国畜牧兽医,2010,37(3):75-78.ZHANG Yan-jun,LI Jin-quan,YIN Jun,et al.(2010).Advances in research on the mechanism of homeobox gene on hair follicle growth and development [J].Chinese Animal Husbandry Veterinarian,37(3):75-78.(in Chinese)
[14] 黄培堂.分子克隆实验指南精编版[J].生物技术通讯,2008,(6):865.HUANG Pei-tang.(2008).Molecular Cloning Experiment Guide [J].Biotechnology Newsletter,(6):865.(in Chinese)
[15] 哈尼克孜·吐拉甫,吴伟伟,徐新明,等.分子遗传标记在绵羊育种中的研究进展[J].新疆畜牧业,2017,(3):13-16.Hanikezi Tulafu,WU Wei-wei,XU Xin-ming,et al.(2017).Advances in molecular genetic markers in sheep breeding [J].Xinjiang Animal Husbandry,(3):13-16.(in Chinese)
[16] 田月珍,黄锡霞,狄江,等.中国美利奴羊(新疆型)皮肤组织GAPDH基因实时荧光定量PCR方法的建立[J].新疆农业科学,2012,49(10):1 938-1 943.TIAN Yue-zhen,HUANG Xi-xia,DI Jiang,et al.(2012).Establishment of Real-time Fluorescence Quantitative PCR Method for GAPDH Gene in Skin Tissue of Chinese Merino Sheep (Xinjiang Type) [J].Xinjiang Agricultural Sciences,49(10):1,938-1,943.(in Chinese)
[17] 陈旭,齐凤坤,康立功,等.实时荧光定量PCR技术研究进展及其应用[J].东北农业大学学报,2010,21(8):148-155.CHEN Xu,QI Feng-kun,KANG Li-gong,et al.(2010).Research progress and application of real-time fluorescent quantitative PCR [J].Journal of Northeast Agricultural University,21(8):148-155.(in Chinese)
[18] 柳楠,卜然,贺建宁,等.Hox基因家族对细毛羊羊毛性状影响的研究[J].中国畜牧杂志,2014,50(23):6-10.LIU Nan,BO Ran,HE Jian-ning,et al.(2014).Effect of Hox Gene Family on Wool Properties of Fine Wool Sheep [J].Chinese Journal of Animal Science,50(23):6-10.(in Chinese)
[19] 阿布来提·苏来曼,田月珍,许琴,等.苏博美利奴羊胎儿皮肤毛囊结构及形态发育[J].中国农业科学,2017,50(16):3 226-3 235.Abulaiti Sulaiman,TIAN Yue-zhen,XU Qin,et al.(2017).Structure and morphological development of fetal hair follicles in Subo Merino sheep [J].Scientia Agricultura Sinica,50(16):3,226-3,235.(in Chinese)
[20] 吴江鸿,闫祖威,胡斯乐,等.Hoxc13在毛囊发育中的作用[J].遗传,2010,32(7):656-662.WU Jiang-hong,YAN Zu-wei,HU Si-le,et al.(2010).Hoxc 13 and the development of hair follicle [J].Hereditas,32(7):656-662.(in Chinese)
[2] 柳楠,田可川,石国庆,等.苏博美利奴羊核心群级进杂交不同世代对产毛性状影响的研究[J].中国畜牧杂志,2015,51(15):6-10.LIU Nan,TIAN ke-chuan,SHI Guo-qing,et al.(2015).Study on the Influence of Different Generations of Subo Merino Sheep Core Group Progressive Hybrids on Hair Growth Traits [J].Chinese Journal of Animal Science,51(15):6-10.(in Chinese)
[3] 田月珍,黄锡霞,柏妍,等.与毛囊生长发育及羊毛、羊绒品质相关基因的研究进展[J].中国草食动物科学,2013,33(3):55-58.TIAN Yue-zhen,HUANG Xi-xia,BAI Yan,et al.(2013).Advances in research on genes related to hair follicle growth and development,wool and cashmere quality [J].Chinese Herbivore Science,33(3):55-58.(in Chinese)
[4] 王宁,荣恩光,闫晓红.毛囊发育与毛发生产研究进展[J].东北农业大学学报,2012,(9):6-12.WANG Ning,RONG En-guang,YAN Xiao-hong,et al.(2012).Research progress on hair follicle development and hair production [J].Journal of Northeast Agricultural University,(9):6-12.(in Chinese)
[5] Rogers,G.E.(2006).Biology of the wool follicle:an excursion into a unique tissue interaction system waiting to be re-discovered.Experimental dermatology,15(12):931-949.
[6] Galbraith,H.(2010).Fundamental hair follicle biology and fine fiber production in animals.Animal,4(9):1,490-1,509.
[7] 吴江鸿.Hoxc13基因在绒山羊皮肤中的表达规律及体外功能分析[D].呼和浩特:内蒙古农业大学,2011.WU Jiang-hong.(2011).Expression of Hoxc13 Gene in Cashmere Goat Skin and Analysis of Its Function in Vitro [D].Master Dissertation.Inner Mongolia Agricultural University,Hohhot.(in Chinese)
[8] 夏辉,李龙江.Hox基因与肿瘤发生发展的研究进展[J].国际口腔医学杂志,2007,34(6):412-414.XIA Hui,LI Long-jiang.(2007).Advances in research on Hox gene and tumorigenesis [J].International Journal of Stomatology,34(6):412-414.(in Chinese)
[9] 金淑清,浦予飞,裘莹.Hox基因的研究进展[J].癌症进展,2011,9(2):154-158.JIN Shu-qing,FU Yu-fei,QIU Ying.(2011).Research progress of Hox gene [J].Cancer Progression,9(2):154-158.(in Chinese)
[10] Yang,M.,Li,Q.F.,& Zhang,F.(2010).Hox genes in the skin.Chinese medical journal,123(18):2,607-2,612.
[11] Hui-Feng,S.,Jia-Ke,C.,& Min-Liang,C..(2005).Differential display of homeobox gene expressions in the normal,wounded human fetal and adult skins by DNA microarray.Chinese Journal of Plastic Surgery & Burns,21(5):372-374.
[12] 白玉妍,范衡宇,杨增明.同源盒基因(Hox)与哺乳动物生殖[J].生物化学与生物物理进展,2001,28(3):329-332.BAI Yu-yan,FAN Heng-yu,YANG Zeng-ming.(2001).Homeobox gene (Hox) and mammalian reproduction [J].Progress in Biochemistry and Biophysics,28(3):329-332.(in Chinese)
[13] 张燕军,李金泉,尹俊,等.Homeobox基因对毛囊生长发育作用机制的研究进展[J].中国畜牧兽医,2010,37(3):75-78.ZHANG Yan-jun,LI Jin-quan,YIN Jun,et al.(2010).Advances in research on the mechanism of homeobox gene on hair follicle growth and development [J].Chinese Animal Husbandry Veterinarian,37(3):75-78.(in Chinese)
[14] 黄培堂.分子克隆实验指南精编版[J].生物技术通讯,2008,(6):865.HUANG Pei-tang.(2008).Molecular Cloning Experiment Guide [J].Biotechnology Newsletter,(6):865.(in Chinese)
[15] 哈尼克孜·吐拉甫,吴伟伟,徐新明,等.分子遗传标记在绵羊育种中的研究进展[J].新疆畜牧业,2017,(3):13-16.Hanikezi Tulafu,WU Wei-wei,XU Xin-ming,et al.(2017).Advances in molecular genetic markers in sheep breeding [J].Xinjiang Animal Husbandry,(3):13-16.(in Chinese)
[16] 田月珍,黄锡霞,狄江,等.中国美利奴羊(新疆型)皮肤组织GAPDH基因实时荧光定量PCR方法的建立[J].新疆农业科学,2012,49(10):1 938-1 943.TIAN Yue-zhen,HUANG Xi-xia,DI Jiang,et al.(2012).Establishment of Real-time Fluorescence Quantitative PCR Method for GAPDH Gene in Skin Tissue of Chinese Merino Sheep (Xinjiang Type) [J].Xinjiang Agricultural Sciences,49(10):1,938-1,943.(in Chinese)
[17] 陈旭,齐凤坤,康立功,等.实时荧光定量PCR技术研究进展及其应用[J].东北农业大学学报,2010,21(8):148-155.CHEN Xu,QI Feng-kun,KANG Li-gong,et al.(2010).Research progress and application of real-time fluorescent quantitative PCR [J].Journal of Northeast Agricultural University,21(8):148-155.(in Chinese)
[18] 柳楠,卜然,贺建宁,等.Hox基因家族对细毛羊羊毛性状影响的研究[J].中国畜牧杂志,2014,50(23):6-10.LIU Nan,BO Ran,HE Jian-ning,et al.(2014).Effect of Hox Gene Family on Wool Properties of Fine Wool Sheep [J].Chinese Journal of Animal Science,50(23):6-10.(in Chinese)
[19] 阿布来提·苏来曼,田月珍,许琴,等.苏博美利奴羊胎儿皮肤毛囊结构及形态发育[J].中国农业科学,2017,50(16):3 226-3 235.Abulaiti Sulaiman,TIAN Yue-zhen,XU Qin,et al.(2017).Structure and morphological development of fetal hair follicles in Subo Merino sheep [J].Scientia Agricultura Sinica,50(16):3,226-3,235.(in Chinese)
[20] 吴江鸿,闫祖威,胡斯乐,等.Hoxc13在毛囊发育中的作用[J].遗传,2010,32(7):656-662.WU Jiang-hong,YAN Zu-wei,HU Si-le,et al.(2010).Hoxc 13 and the development of hair follicle [J].Hereditas,32(7):656-662.(in Chinese)