蓝藻的蛋白质翻译后修饰研究进展
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摘要
蛋白质翻译后修饰系统几乎参与了细胞所有的正常生命活动过程,并发挥着重要的调控作用。目前,基于生物质谱技术进行蛋白质翻译后修饰的规模化分析鉴定,已经成为蛋白质组学研究的核心内容之一。近年来的研究表明,蓝藻细胞中存在着复杂的蛋白质翻译后修饰系统,如磷酸化,乙酰化,甲基化,糖基化,氧化等,这些翻译后修饰在蓝藻细胞的代谢过程中可能发挥着重要的调控作用。本文主要针对蓝藻细胞中蛋白质翻译后修饰的发现与鉴定,以及翻译后修饰潜在的生物学功能展开简要综述。
Post-translational modifications(PTMs) are vital cellular control mechanisms modulating diverse protein properties. Proteomic analysis of post-translational modifications based on the mass spectrometry has been the key research area. Recently, it has been shown that protein post-translational modifications, such as phosphorylation, acetylation, methylation, glycosylation and oxidation, play crucial regulatory role in various pathways of cyanobacteria. In the present article, we reviewed the recent progress on the functional studies of PTMs in cyanobacteria.
Post-translational modifications(PTMs) are vital cellular control mechanisms modulating diverse protein properties. Proteomic analysis of post-translational modifications based on the mass spectrometry has been the key research area. Recently, it has been shown that protein post-translational modifications, such as phosphorylation, acetylation, methylation, glycosylation and oxidation, play crucial regulatory role in various pathways of cyanobacteria. In the present article, we reviewed the recent progress on the functional studies of PTMs in cyanobacteria.
引文
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[24]Mikkat S,Fulda S,Hagemann M.A 2D gel electrophoresis-based snapshot of the phosphoproteome in the cyanobacterium Synechocystis sp.strain PCC 6803[J].Microbiology,2014,160:296-306
[25]Spat P,Macek B,Forchhammer K.Phosphoproteome of the cyanobacterium Synechocystis sp PCC 6803 and its dynamics during nitrogen starvation[J].Frontiers in Microbiology,2015,6
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