蛹虫草中游离糖醇、小分子糖类成分研究
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摘要
建立高效阴离子色谱-脉冲安培法同时测定赤藓糖醇、阿糖醇、海藻糖、甘露醇和葡萄糖的方法 ,方法学考察结果表明该方法精密度(1.4%~4.4%)、稳定性(2.1%~4.9%)、重复性(0.6%~4.5%)和回收率(95.2%~105.7%)良好。用该方法对13个蛹虫草(Cordyceps militaris)子实体和4个菌丝体样品中游离糖醇、小分子糖类成分进行分析,结果表明蛹虫草子实体中含海藻糖(1.04%~19.17%)、甘露醇(0.96%~3.93%)和葡萄糖(1.46%~14.93%),其中海藻糖和葡萄糖含量呈显著负相关;菌丝体中含甘露醇(3.66%~6.70%)和葡萄糖(0.42%~1.06%)以及少量阿糖醇(0.02%~0.12%)。
An accurate and reliable method for the simultaneous determination of erythritol,arabitol,mannitol,trehalose and glucose is described.Relative Standard Deviation(RSD)values for precision,stability,reproducibility and recovery ranged between 1.4%-4.4%,2.1%-4.9%,0.6%-4.5% and 84.9%-108.3%,respectively.Free alditol and low molecular sugar levels in 13 samples of Cordyceps militaris fruit bodies determined using this method ranged between 1.04%-19.17%(trehalose),0.96%-3.93%(mannitol)and 1.46%-14.93%(glucose)dry weight,and there was a significant negative correlation(-0.83)between trehalose and glucose content.Mannitol,glucose and arabitol levels in four samples of C.militaris mycelia grown in submerged culture ranged between 3.66%-6.70%,0.42%-1.06% and 0.02%-0.12% dry weight,respectively.
An accurate and reliable method for the simultaneous determination of erythritol,arabitol,mannitol,trehalose and glucose is described.Relative Standard Deviation(RSD)values for precision,stability,reproducibility and recovery ranged between 1.4%-4.4%,2.1%-4.9%,0.6%-4.5% and 84.9%-108.3%,respectively.Free alditol and low molecular sugar levels in 13 samples of Cordyceps militaris fruit bodies determined using this method ranged between 1.04%-19.17%(trehalose),0.96%-3.93%(mannitol)and 1.46%-14.93%(glucose)dry weight,and there was a significant negative correlation(-0.83)between trehalose and glucose content.Mannitol,glucose and arabitol levels in four samples of C.militaris mycelia grown in submerged culture ranged between 3.66%-6.70%,0.42%-1.06% and 0.02%-0.12% dry weight,respectively.
引文
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[2]蒙健宗,马少敏.杏鲍菇中海藻糖和多糖含量分析[J].食品工业科技,2011,32(8):174-175,245.
[3]段秀辉,李露,薛淑静,等.杏鲍菇、香菇及其预煮液中可溶性糖的GC-MS分析[J].食品工业科技,2015,36(17):281-285.
[4]周帅,薛俊杰,刘艳芳,等.高效阴离子色谱一脉冲安培检测法分析食用菌中海藻糖、甘露醇和阿糖醇[J].食用菌学报,2011,18(1):49-52.
[5]周帅,薛俊杰,贾薇,等.食用菌菌丝体中糖醇类成分含量测定的研究[J].菌物学报,2013,32(5):862-867.
[6]刘艳芳,唐庆九,顾俊杰.北冬虫夏草深层发酵高产虫草素工艺的优化[J].上海农业学报,2010,26(3):26-30.
[7]邵颖,李文,王陶.蛹拟青霉发酵菌丝体中虫草酸的提取与测定[J].食品工业科技,2012,33(1):262-267.
[8]蔡友华,范文霞,刘学铭.比色法测定巴西虫草菌丝体中虫草酸的含量[J].现代食品科技,2008,24(1):76-79.