黄酮化合物对耐药性鲍曼不动杆菌抑菌作用研究
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摘要
为探索中药黄酮化合物对耐药性鲍曼不动杆菌的抑菌效果。研究通过微量肉汤稀释法,考察40种中药及天然来源的黄酮化合物对三株耐药性鲍曼不动杆菌菌株的体外抑菌作用,测试其最低抑菌浓度与最低杀菌浓度;采用结晶紫染色法进一步测定目标化合物对鲍曼不动杆菌生物被膜形成的能力;探索黄酮联合头孢哌酮/舒巴坦对鲍曼不动杆菌的抑菌效果。研究表明,黄酮单体对于鲍曼不动杆菌具有不同程度的体外抑菌作用。其中部分具有轻微作用,其最小抑菌浓度仅为0.5 mg/mL;黄芩素和汉黄芩苷具有显著的抑菌作用,其最小抑菌浓度分别为0.062 5和0.125 mg/mL;同时,黄芩素对鲍曼不动杆菌生物被膜形成具有明显的抑制作用。此外,黄芩素和头孢哌酮/舒巴坦联合作用于耐药鲍曼不动杆菌与单用黄芩素或头孢哌酮/舒巴坦比较,浓度有所降低。综上,黄芩素对耐药性鲍曼不动杆菌有较好的体外抑菌效果,这为治疗耐药性鲍曼不动杆菌引发的感染的治疗提供新思路和方向。
To explore the antibacterial effects of flavonoids on resistant Acinetobacter baumannii.In this research,the antibacterial effects and the minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) of 40 kinds of traditional Chinese medicine flavonoids on three strains of Acinetobacter baumannii were tested by micro broth dilution method in vitro.Biofilm-forming ability was detected by crystal violet staining assay.The antimicrobial tests of flavonoids combined with Cefoperazone/sulbactam were also conducted.The flavonoids showed antibacterial effect in vitro at different degrees on Acinetobacter baumannii.Most of them have faint inhibitions,and their MICs were 0.5 mg/mL.Baicalein and wogonoside have significant antibacterial effect,of which the mean MICs were 0.062 5 and 0.125 mg/mL,respectively.Meanwhile,baicalein also showed obvious inhibitory effect on biofilm formation.Besides,inhibitory effect of baicalein combined with Cefoperazone/sulbactam on resistant Acinetobacter baumannii reduced compared with single baicalein or cefoperazone/sulbactam.In summary,baicalein has a good antibacterial effect on drug resistant Acinetobacter baumannii,which provides new ideas and directions for the treatment of resistant Acinetobacter baumannii infection.
To explore the antibacterial effects of flavonoids on resistant Acinetobacter baumannii.In this research,the antibacterial effects and the minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) of 40 kinds of traditional Chinese medicine flavonoids on three strains of Acinetobacter baumannii were tested by micro broth dilution method in vitro.Biofilm-forming ability was detected by crystal violet staining assay.The antimicrobial tests of flavonoids combined with Cefoperazone/sulbactam were also conducted.The flavonoids showed antibacterial effect in vitro at different degrees on Acinetobacter baumannii.Most of them have faint inhibitions,and their MICs were 0.5 mg/mL.Baicalein and wogonoside have significant antibacterial effect,of which the mean MICs were 0.062 5 and 0.125 mg/mL,respectively.Meanwhile,baicalein also showed obvious inhibitory effect on biofilm formation.Besides,inhibitory effect of baicalein combined with Cefoperazone/sulbactam on resistant Acinetobacter baumannii reduced compared with single baicalein or cefoperazone/sulbactam.In summary,baicalein has a good antibacterial effect on drug resistant Acinetobacter baumannii,which provides new ideas and directions for the treatment of resistant Acinetobacter baumannii infection.
引文
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2 Kumarasamy KK,Toleman MA,Walsh TR,et al.Emergence of a new antibiotic resistance mechanism in India,Pakistan,and the UK:A molecular,biological,and epidemiological study[J].Lancet Infect Dis,2010,10:597-602.
3 Brown ED,Wright GD.Antibacterial drug discovery in the resistance era[J].Nature,2016,529:336-343.
4 Bush K,Pucci MJ.New antimicrobial agents on the horizon[J].Biochem Pharmacol,2011,82:1528-1539.
5 Liu QP,Xu L.Advances in resistance mechanisms of drug-resistant Acinetobacter baumannii[J].Chin J Antibiot(中国抗生素杂志),2018,43:1179-1187.
6 Liu QP,Xu L.Research progress of novel treatments targeting drug-resistant Acinetobacter baumannii[J].Chin J Infect Chemother(中国感染与化疗杂志),2018,18:216-220.
7 Ruan XM,Shi DH.The antibacterial effects of herb monomers:Research advances[J].Chin J Microecol(中国微生态学杂志),2015,27:244-248.
8 Liu L,Lin H,Huang RS,et al.Study on inhibitory effect of baicalin and baicalein on the formation of Streptococcus mutans biofilm[J].Tradit Chin Drug Res Clin Pharmacol(中药新药与临床药理),2017,28:464-467.
9 Yang CQ,YangWJ,Liu J.Advances on chemical ecology of plant flavonoids[J].Nat Prod Res Dev(天然产物研究与开发),2018,30:2009-2016.
10 Ma DM,Tao QC,Qi HW.Research of antibacterial effect of Shuanghuanglian of Chinese medicine in vitro on acinetobacter baumannii of pan-resistant drugs[J].J Clin Med Pract(实用临床医药杂志),2014,18:109-111.
11 Fu RQ,Lu LC,Li ZH,et al.Antibacterial effects of 31 kinds of traditional Chinese medicine monomers on MRSA in vitro[J].China Pharm(中国药业),2014,23(4):20-22.
12 Chai F,Li JY,Zhang HF,et al.Skullcap capsule on bacteriostasis in vitro of 193 strains of multi-drug resistant Acinetobacter baumannii[J].Chin Arch Tradit Chin Med(中华中医药学刊),2016,34:2694-2696.
13 Cao X,He WS,Wang N,et al.Detection of antibacterial effects by 6 kinds of traditional Chinese medicine as Scutellaria baicalensis for 60 Acinetobacter baumannii strains C[J].China Prac Med(中国实用医药),2016,11(30):19-20.
14 Lai QW,Huang LF.Acinetobacter baumannii biofilm and antimicrobial resistance correlation analysis[J].Int J Lab Med(国际检验医学杂志),2016,37:153-154.