宁波大榭口岸鼠类携带细菌调查
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摘要
目的调查宁波大榭口岸鼠类携带细菌的情况。方法用鼠笼鼠夹法在大榭口岸捕鼠,对鼠类肠组织进行细菌16S rDNA的扩增、克隆和测序;采用细菌分离和生化鉴定法进行细菌种属鉴定。结果 2017年5月—2018年4月,从大榭口岸4个生境中捕获71只鼠类,检出201株细菌,包含粪肠球菌、希氏肠球菌、松鼠葡萄球菌、短小芽胞杆菌等17种;克隆测序法鉴定到16种细菌,分离培养法鉴定到9种。结论掌握了大榭口岸鼠类携带细菌情况,建立了菌种库。
Objective To analyze the distribution of bacteria in mice by collecting samples from Daxie port.Methods The 16 S rDNA was amplified,cloned and sequenced from bacteria in the intestinal tissues of mice captured at Daxie port. The identification of bacterial species was carried out by biochemical method of bacterial isolation. Results From May 2017 to April 2018,71 mice were collected from 4 habitats at Daxie port,and 201 strains of bacteria were detected,belonging to 17 species,including Enterococcus faecalis,Enterococcus hidalis,Staphylococcus squirrel,and Bacillus parvus. Among them,16 species were obtained by clonal sequencing, 9 species were isolated and cultured. Conclusion The situation of rodents carrying bacteria in Daxie port was mostered,and the germ bank was established.
Objective To analyze the distribution of bacteria in mice by collecting samples from Daxie port.Methods The 16 S rDNA was amplified,cloned and sequenced from bacteria in the intestinal tissues of mice captured at Daxie port. The identification of bacterial species was carried out by biochemical method of bacterial isolation. Results From May 2017 to April 2018,71 mice were collected from 4 habitats at Daxie port,and 201 strains of bacteria were detected,belonging to 17 species,including Enterococcus faecalis,Enterococcus hidalis,Staphylococcus squirrel,and Bacillus parvus. Among them,16 species were obtained by clonal sequencing, 9 species were isolated and cultured. Conclusion The situation of rodents carrying bacteria in Daxie port was mostered,and the germ bank was established.
引文
[1]国家质检总局. SN/T 1240-2003国境口岸鼠类监测规程[S].北京:中国标准出版社,2012.
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[6]蒋正杰,苗凤英,齐宗倩,等.1998年北京东城区痢疾杆菌和病原性大肠杆菌菌型分布及药敏实验报告[J].中国公共卫生,2000,17(1):72.
[7]胡立芬,程君,李家斌,等.安徽省志贺菌属菌群分布及耐药性研究[J].中华医院感染学杂志,2007,17(2):218-219.
[2] Jin CY,Zeng ZY,Fu ZW,et al. Oral imazalil exposure induces gut microbiota dysbiosis and colonic inflammation in mice[J].Chemosphere,2016(160):349-358.
[3] Valenstein P,Pfaller M,Yungbluth M. The use and abuse of routine stool microbiology:a college of American pathologists Q-probes study of 601 institutions[J]. Arch Pathol Lab Med,1996(120):206-211.
[4]陆德源.医学微生物学.第5版[M].北京:人民卫生出版社,2001.
[5] Turner SA,Luck SN,Sakellaris H,et al,Role of attP in integrase-mediated integration of the Shigella resistance locus pathogenicily island of Shigella flexneri[J]. Antimicrob Agents Chemother,2004,48(3):1028-1031.
[6]蒋正杰,苗凤英,齐宗倩,等.1998年北京东城区痢疾杆菌和病原性大肠杆菌菌型分布及药敏实验报告[J].中国公共卫生,2000,17(1):72.
[7]胡立芬,程君,李家斌,等.安徽省志贺菌属菌群分布及耐药性研究[J].中华医院感染学杂志,2007,17(2):218-219.