竹子根际螺旋线虫中国新记录种—尖尾螺旋线虫Helicotylenchus cuspicaudatus
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摘要
在广东省园林植物线虫调查期间,从粉单竹Bambusa chungii根部土壤中分离到一种螺旋线虫。经详细的形态学观察和测量数据比较,将其鉴定为尖尾螺旋线虫Helicotylenchus cuspicaudatus,并获得了尖尾螺旋线虫rDNA的28SD2-D3区和ITS序列,为今后螺旋线虫的种类鉴定提供了分子数据。尖尾螺旋线虫为中国新记录种。
A species of spiral nematode was isolated from the rhizosphere soil of Bambusa chungii during the survey of parasitic nematodes associated with landscape plants in Guangdong Province.The detailed morphological study confirmed the species as Helicotylenchus cuspicaudatus.The fragments of rDNA 28S D2-D3 and ITS were amplified and sequenced,which provided molecular characters for rapid and accurate identification of this species in the future.H.cuspicaudatus was reported in China for the first time.
A species of spiral nematode was isolated from the rhizosphere soil of Bambusa chungii during the survey of parasitic nematodes associated with landscape plants in Guangdong Province.The detailed morphological study confirmed the species as Helicotylenchus cuspicaudatus.The fragments of rDNA 28S D2-D3 and ITS were amplified and sequenced,which provided molecular characters for rapid and accurate identification of this species in the future.H.cuspicaudatus was reported in China for the first time.
引文
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[9]VRAIN T C,WAKARCHUK D A,LEVESQUE A C,et al.Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group[J].Fundamental and Applied Nematology,1992,15(6):563-573.
[10]SUBBOTIN S A,VOVLAS N,YEATES G W,et al.Morphological and molecular characterization of Helicotylenchus pseudorobustus(Steiner,1914)Golden,1956and related species(Tylenchida:Hoplolaimidae)with phylogeny of the genus[J].Nematology,2015,17(1):25-52.
[11]SAHA M,LAL M,SINGH M,et al.Four new species of Hoplolaimoidea(Nematoda:Tylenchida)from India[J].International Journal of Nematology,2000,10(2):192-198.
[12]SUBBOTIN S A,STURHAN D,VOVLAS N,et al.Application of the secondary structure model of rRNA for phylogeny:D2-D3expansion segments of the LSU gene of plant-parasitic nematodes from the family Hoplolaimidae Filipjev,1934[J].Molecular Phylogenetics&Evolution,2007,43(3):881-890.
[13]周纪刚,舒夏竺,徐平,等.广东粉单竹病虫害调查及防治[J].林业实用技术,2014(7):47-49.
[2]DAVIS L T,BELL N L,WATSON R N,et al.Host range assessment of Helicotylenchus pseudorobustus(Tylenchida:Hoplolaimidae)on pasture species[J].Journal of Nematology,2004,36(4):487.
[3]UZMA I,NASIRA K,FIROZA K,et al.Review of the genus Helicotylenchus Steiner,1945(Nematoda:Hoplolaimidae)with updated diagnostic compendium[J].Pakistan Journal of Nematology,2015,33(2):115-160.
[4]刘维志.植物线虫志[M].北京:中国农业出版社,2004.
[5]冯志新.植物线虫学[M].北京:中国农业出版社,2001.
[6]WANG H,ZHUO K,YE W,et al.Morphological and molecular charaterisation of Pratylenchus parazeae n.sp.(Nematoda:Pratylenchidae)parasitizing sugarcane in China[J].European Journal of Plant Pathology,2015,143(1):173-191.
[7]MUNDO-OCAMPO M,TROCCOLI A,SUBBOTIN S A,et al.Synonymy of Afenestrata with Heterodera supported by phylogenetics with molecular and morphological characterisation of H.koreanacomb.n.and H.orientalis comb.n.(Tylenchida:Heteroderidae)[J].Nematology,2008,10(5):611-632.
[8]SUBBOTIN S A,STURHAN D,CHIZHOV V N,et al.Phylogenetic analysis of Tylenchida Thorne,1949as inferred from D2and D3expansion fragments of the 28SrRNA gene sequences[J].Nematology,2006,8(3):455-474.
[9]VRAIN T C,WAKARCHUK D A,LEVESQUE A C,et al.Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americanum group[J].Fundamental and Applied Nematology,1992,15(6):563-573.
[10]SUBBOTIN S A,VOVLAS N,YEATES G W,et al.Morphological and molecular characterization of Helicotylenchus pseudorobustus(Steiner,1914)Golden,1956and related species(Tylenchida:Hoplolaimidae)with phylogeny of the genus[J].Nematology,2015,17(1):25-52.
[11]SAHA M,LAL M,SINGH M,et al.Four new species of Hoplolaimoidea(Nematoda:Tylenchida)from India[J].International Journal of Nematology,2000,10(2):192-198.
[12]SUBBOTIN S A,STURHAN D,VOVLAS N,et al.Application of the secondary structure model of rRNA for phylogeny:D2-D3expansion segments of the LSU gene of plant-parasitic nematodes from the family Hoplolaimidae Filipjev,1934[J].Molecular Phylogenetics&Evolution,2007,43(3):881-890.
[13]周纪刚,舒夏竺,徐平,等.广东粉单竹病虫害调查及防治[J].林业实用技术,2014(7):47-49.