人易感H10N8禽流感病毒血凝素蛋白主要特性及B/T细胞抗原表位预测分析
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摘要
目的应用生物信息学方法分析人H10N8禽流感病毒血凝素蛋白(hemagglutinin,HA)的主要特性,预测可能的B/T细胞抗原表位。方法采用Prot Param预测H10N8禽流感病毒HA蛋白的理化特性,SOPMA预测二级结构,Motif Scan预测翻译后修饰位点,DNAStar分析其序列的亲水性指数、柔韧性指数、可及性参数以及抗原指数,推测B细胞抗原表位的可能位置,并采用SYFPEITHI的T细胞表位预测工具分别预测CTL和Th细胞表位。结果人易感H10N8禽流感病毒HA为亲水性蛋白,二级结构中包含218个α-螺旋、108个β-片层、64个β-转角和171个无规卷曲,具有多个糖基化、酰胺化及磷酸化翻译后修饰位点。经综合各种参数,预测出H10N8禽流感病毒HA蛋白可能存在的7个B细胞抗原表位、7个CTL细胞表位和8个Th细胞表位。结论 HA蛋白B/T细胞抗原表位的预测为人易感H10N8染禽流感病毒的疫苗研制奠定了一定的基础。
Objective To predict the main characteristics and B/T cell epitopes of hemagglutinin( HA) of human-infecting avian influenza A( H10 N8). Methods The physical and chemical properties were predicted by using Prot Param,and secondary structure was predicted by using SOPMA online server. The post translation sites were predicted by using Motif Scan software. The B cell epitopes were analyzed by DNAStar software,which can analyze the hydrophilicity,flexible regions,surface accessibility regions and antigenic index. The T cell epitopes were analyzed by SYFPEITHI. Results HA of influenza A( H10 N8) was a hydrophilic protein,which had 218 α-helixs,108 β-sheets,64 β-turns and 171 random coils,and also had some post-translational modification sites. In addition,7 potential B cell epitopes,7 potential CTL cell epitopes and 8 potential Th cell epitopes of HA protein were predicted. Conclusion The B/T cell epitopes prediction might be useful to develop some promising anti-H10 N8 vaccines based on the peptide.
Objective To predict the main characteristics and B/T cell epitopes of hemagglutinin( HA) of human-infecting avian influenza A( H10 N8). Methods The physical and chemical properties were predicted by using Prot Param,and secondary structure was predicted by using SOPMA online server. The post translation sites were predicted by using Motif Scan software. The B cell epitopes were analyzed by DNAStar software,which can analyze the hydrophilicity,flexible regions,surface accessibility regions and antigenic index. The T cell epitopes were analyzed by SYFPEITHI. Results HA of influenza A( H10 N8) was a hydrophilic protein,which had 218 α-helixs,108 β-sheets,64 β-turns and 171 random coils,and also had some post-translational modification sites. In addition,7 potential B cell epitopes,7 potential CTL cell epitopes and 8 potential Th cell epitopes of HA protein were predicted. Conclusion The B/T cell epitopes prediction might be useful to develop some promising anti-H10 N8 vaccines based on the peptide.
引文
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[2]谭伟,谢芝勋.H10N8亚型禽流感病毒的研究进展[J].中国畜牧兽医,2014,41(8):236-241.
[3]CHEN H,YUAN H,GAO R,et al.Clinical and epidemiological characteristics of a fatal case of avian influenza A H10N8 virus infection:a descriptive study[J].Lancet,2014,383(9918):714-721.
[4]WANG X,SUN Q,YE Z,et al.Computational approach for predicting the conserved B-cell epitopes of hemagglutinin H7 subtype influenza virus[J].Experimental and therapeutic medicine,2016,12(4):2439-2446.
[5]WILKINS MR,GASTEIGER E,BAIROCH A,et al.Protein identification and analysis tools in the Ex PASy server[J].Methods Mol Biol,1999,112(112):531-552.
[6]COMBET C,BLANCHET C,GEOURJON C,et al.NPS@:network protein sequence analysis[J].Trends in biochemical sciences,2000,25(3):147-150.
[7]NIKITINA TV,TISHCHENKO LI.Computational search for potential post-translational modification sites in human RNA polymerase III subunits[J].Molekuliarnaia biologiia,2005,39(3):437-444.
[8]KYTE J,DOOLITTLE RF.A simple method for displaying the hydropathic character of a protein[J].Journal of molecular biology,1982,157(1):105-132.
[9]VIHINEN M,TORKKILA E,RIIKONEN P.Accuracy of protein flexibility predictions[J].Proteins,1994,19(2):141-149.
[10]EMINI EA,HUGHES JV,PERLOW DS,et al.Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide[J].Journal of virology,1985,55(3):836-839.
[11]JAMESON BA,WOLF H.The antigenic index:a novel algorithm for predicting antigenic determinants[J].Computer applications in the biosciences cabios,1988,4(1):181-186.
[12]WANG F,YE B.In silico cloning and B/T cell epitope prediction of triosephosphate isomerase from Echinococcus granulosus[J].Parasitology research,2016,115(10):3991-3998.
[13]JIAO P,CAO L,YUAN R,et al.Complete genome sequence of an H10N8 avian influenza virus isolated from a live bird market in Southern China[J].Journal of virology,2012,86(14):7716.
[14]IMAI M,WATANABE T,HATTA M,et al.Experimental adaptation of an influenza H5 HA confers respiratory droplet transmission to a reassortant H5HA/H1N1 virus in ferrets[J].Nature,2012,486(7403):420-428.
[15]ZHANG T,BI Y,TIAN H,et al.Human infection with influenza virus A(H10N8)from live poultry markets,China,2014[J].Emerging infectious diseases,2014,20(12):2076-2079.
[16]EL-MANZALAWY Y,HONAVAR V.Recent advances in B-cell epitope prediction methods[J].Immunome research,2010,6(S2):1-9.
[17]杨依丽,吴军.病毒抗原糖基化与免疫关系的研究进展[J].生物技术通讯,2008,19(5):728-730.