微柱凝胶技术检测血型抗体IgG亚型的应用评价
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摘要
目的:评价微柱凝胶技术用于检测孕妇血型抗体IgG亚型的可靠性以及IgG亚型与新生儿溶血病的相关性。方法:采用自主研发的IgG亚型微柱凝胶反应卡,对47例产前夫妇抗体效价高的孕妇测定其IgG亚类抗体的效价,其中ABO血型系统38例,Rh系统9例,对产后出现溶血病的患儿亦监测其IgG亚型抗体水平,35例抗体效价正常的孕妇及其产后新生儿作为对照组。结果:47例血型抗体高效价的孕妇血清中检测出26例IgG1和IgG3亚型同步升高,产后新生儿均出现了不同程度的高胆红素血症,21例IgG2和IgG4亚型升高,产后新生儿未出现高胆红素血症表现。结论:微柱凝胶技术用于检测孕妇血型抗体IgG亚型,方法简单快速、结果直观可靠,可以在普通实验室常规应用,对高效价孕妇有必要进行IgG亚型分析,当检出IgG1或IgG3亚型升高时给予预防性干预治疗,而如果仅IgG2或IgG4亚型升高,孕妇可以不进行早期干预治疗,从而实现精准化干预治疗。
Objective:To evaluate the reliability of the micro column gel technique assay(MGTA)in the detection of blood type antibodies IgG subsets and the relationships between neonatal hemolytic disease and the IgG subsets.Method:The independent innovative method of microcolum gel technology in IgG subset detection was established.The IgG subsets were analyzed on 47 cases pregnant women(ABO 38 cases and Rh 9 cases)who had the high titer antibodies and the neonatal sera of HDN.At the same time 35 cases healthy gravidaes and their infants were control group.Result:There were 26 pregnant women in totle 47 cases,and their concentration of IgG1 or IgG3 were higher than those in normal group(P<0.05),neonates were vulnerable to HDN.It was certificated the21 pregnant women with IgG2 or IgG4,and neonates were not vulnerable to HDN.Conclusion:The MGTA may have the advantages of easy procedure and high accuracy in screening the contents of IgG subsets to be propitious for general clinical application.The method may provide a directional significance in early intervention of blood group incompatibility hemolytic disease of the newborns.
Objective:To evaluate the reliability of the micro column gel technique assay(MGTA)in the detection of blood type antibodies IgG subsets and the relationships between neonatal hemolytic disease and the IgG subsets.Method:The independent innovative method of microcolum gel technology in IgG subset detection was established.The IgG subsets were analyzed on 47 cases pregnant women(ABO 38 cases and Rh 9 cases)who had the high titer antibodies and the neonatal sera of HDN.At the same time 35 cases healthy gravidaes and their infants were control group.Result:There were 26 pregnant women in totle 47 cases,and their concentration of IgG1 or IgG3 were higher than those in normal group(P<0.05),neonates were vulnerable to HDN.It was certificated the21 pregnant women with IgG2 or IgG4,and neonates were not vulnerable to HDN.Conclusion:The MGTA may have the advantages of easy procedure and high accuracy in screening the contents of IgG subsets to be propitious for general clinical application.The method may provide a directional significance in early intervention of blood group incompatibility hemolytic disease of the newborns.
引文
[1]蔡晓红,王学锋.胎儿新生儿溶血病的实验诊断[J].诊断学理论与实践,2015,14(6):507-509.
[2]刘光箭.新生儿溶血病预报与产前孕妇IgG抗体分析[J].临床血液学杂志,2015,28(10):900-902.
[3]朱碎永,朱燕英,林甲进,等.新生儿ABO溶血病患儿红细胞检测其血液中IgG亚型的应用[J].中国卫生检验杂志,2016,26(8):1154-1155.
[4]马印图,王更银,李玉秋,等.微柱凝胶技术检测血型抗体IgG亚型试验方法的建立[J].临床血液学杂志,2018,31(4):253-256.
[5]龚君顺,沈雨青,苏奶助,等.孕妇检测不规则抗体在非ABO新生儿溶血病中的临床意义[J].临床血液学杂志,2015,28(10):871-872.
[6]张趁利,庞桂芝,娄白敏[J].孕妇产前血型抗体效价与新生儿溶血病的检测结果分析[J].临床血液学杂志,2018,31(2):124-126.
[7]刘光箭.新生儿溶血病预报与产前孕妇IgG抗体分析[J].临床血液学杂志,2015,28(10):900-902.
[8]蔡忠鹤,遇红梅,刘铁梅.孕妇血型IgG抗体效价与新生儿溶血病发病率关系的分析[J].中国实验诊断学,2015,19(4):629-631.
[9] Kagan KO,Eiben B,Kozlowski P.Combined first trimester screening and cell-free fetal DNA-“next generation screening”[J].Ultraschall Med,2014,35:229-236.
[10]张泉,周金安.6000例新生儿溶血病标本血型抗体分析[J].临床血液学杂志,2017,30(12):975-976.
[11]王秀华,彭春花,丁嫦娥,等.孕妇血清中IgG型抗A(B)检测的临床意义[J].临床血液学杂志,2014,27(4):316-317.
[12]陈明,陈小鹤,曾钰.孕妇IgG抗体效价与新生儿溶血病发病的相关研究[J].中国卫生检验杂志,2018,28(4):432-434.
[2]刘光箭.新生儿溶血病预报与产前孕妇IgG抗体分析[J].临床血液学杂志,2015,28(10):900-902.
[3]朱碎永,朱燕英,林甲进,等.新生儿ABO溶血病患儿红细胞检测其血液中IgG亚型的应用[J].中国卫生检验杂志,2016,26(8):1154-1155.
[4]马印图,王更银,李玉秋,等.微柱凝胶技术检测血型抗体IgG亚型试验方法的建立[J].临床血液学杂志,2018,31(4):253-256.
[5]龚君顺,沈雨青,苏奶助,等.孕妇检测不规则抗体在非ABO新生儿溶血病中的临床意义[J].临床血液学杂志,2015,28(10):871-872.
[6]张趁利,庞桂芝,娄白敏[J].孕妇产前血型抗体效价与新生儿溶血病的检测结果分析[J].临床血液学杂志,2018,31(2):124-126.
[7]刘光箭.新生儿溶血病预报与产前孕妇IgG抗体分析[J].临床血液学杂志,2015,28(10):900-902.
[8]蔡忠鹤,遇红梅,刘铁梅.孕妇血型IgG抗体效价与新生儿溶血病发病率关系的分析[J].中国实验诊断学,2015,19(4):629-631.
[9] Kagan KO,Eiben B,Kozlowski P.Combined first trimester screening and cell-free fetal DNA-“next generation screening”[J].Ultraschall Med,2014,35:229-236.
[10]张泉,周金安.6000例新生儿溶血病标本血型抗体分析[J].临床血液学杂志,2017,30(12):975-976.
[11]王秀华,彭春花,丁嫦娥,等.孕妇血清中IgG型抗A(B)检测的临床意义[J].临床血液学杂志,2014,27(4):316-317.
[12]陈明,陈小鹤,曾钰.孕妇IgG抗体效价与新生儿溶血病发病的相关研究[J].中国卫生检验杂志,2018,28(4):432-434.