浸渍法提取薏苡仁最佳工艺条件的研究
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摘要
目的研究浸渍法提取薏苡仁的最佳工艺。方法根据单因素实验及正交实验设计对所提取工艺进行优化设计,以提取药液的粒度、料液比、时间、温度为主要影响因素,以薏苡仁浸膏量为评价指标,采用L9(34)正交实验优选最佳工艺。结果影响薏苡仁浸膏量最佳工艺的先后次序为:粒度>温度>料液比>作用时间,其中粒度影响最大。结论浸渍法提取薏苡仁浸膏最佳乙醇浓度为70%,最佳工艺为A2B3C2D3,与最初的验证实验结果一致,该实验方法单简合理,结果稳定可靠。
Objective In order to obtain optimization of soak method of CoixSeed. Methods According to the single factor experiment and orthogonal experiment design, the extraction process was optimized. The effect of the particle size,solid-liquid ratio,extraction time and extraction temperature of the CoixSeed by single factor experiments and orthogonal experiments in alcohol were investigated. Results The decreasing order of importance of the four process conditions affecting CoixSeed extraction was particle size,extraction temperature, solid-liquid ratio and extraction time,especially particle size effect. Conclusion The best extraction of Coix seed extract concentration of 70% alcoholand the best extraction condition was A2 B3 C2 D3, The method is simple and reasonable,and the results are stable and reliable.
Objective In order to obtain optimization of soak method of CoixSeed. Methods According to the single factor experiment and orthogonal experiment design, the extraction process was optimized. The effect of the particle size,solid-liquid ratio,extraction time and extraction temperature of the CoixSeed by single factor experiments and orthogonal experiments in alcohol were investigated. Results The decreasing order of importance of the four process conditions affecting CoixSeed extraction was particle size,extraction temperature, solid-liquid ratio and extraction time,especially particle size effect. Conclusion The best extraction of Coix seed extract concentration of 70% alcoholand the best extraction condition was A2 B3 C2 D3, The method is simple and reasonable,and the results are stable and reliable.
引文
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[12]Xing Liu,Xin Zhang,Yu-Zhi Rong,et al.Rapid Determination of Fat,Protein and Amino Acid Content in Coix Seed Using Near-Infrared Spectroscopy Technique[J].Food Analytical Methods,2015,8(2):334-342.
[13]Liu Y,Zhang W,Wang XJ,et al.Antitumor effect of Kanglaite?injection in human pancreatic cancer xenografts[J].BMC Complement Altern Med,2014,14(3)228-234.
[14]Xiaoxiang Du,Huizhou Ye,Chunxia Zhang,et al.Effect of kanglaite on rat cytochrome P450[J].Pharmaceutical Biology,2014,53(7),995-1001.
[15]Julien Boccard,Serge Rudaz.Exploring Omics data from designed experiments using Analysis of Variance Multiblock Orthogonal Partial Least Squares[J].Analytica Chimica Acta,2016,920(12):18-28.
[2]Wang L,Chen C,Su A,et al.Structural characterization of phenolic compounds and antioxidant activity of the phenolic-rich fraction from defatted adlay(Coixlachryma-jobi L.var.ma-yuen Stapf)seed meal[J].Food Chem,2016,196(3):509-517.
[3]Huang X,Qin J,Lu S,Kanglaite stimulates anticancer immune responses and inhibits HepG2 cell transplantation.induced tumor growth[J].Mol Med Rep,2014,10(4):2153-2159.
[4]刘静姝,周寒静,隆姝孜,等.薏苡仁酯对同源不同辐射抗性鼻咽癌细胞增殖及凋亡的作用[J].基因组学与应用生物学,2018,37(8):3587-3592.
[5]Jiang YR,Zhang ZH,Ding DM,et al.Study on porous maize starch preparation and powdering coix seed oil[J].Zhongguo Zhong Yao Za Zhi,2013,38(14):2287-2291.
[6]岳莉,赵文英,文佑英,等.薏苡仁油提取工艺优化及动力学模型研究[J].化学工业与工程,2011,28(4):26-29.
[7]巩晓杰,孟宪生,包永睿,等.薏苡仁中酯类化学物质组提取工艺研究[J].中国医药导报,2010,33(7):15-17.
[8]Shi J,Liu Y,Guo R,et al.Design of a New Concentration Series for the Orthog onal Sample Design Approach and Estimation of the Number of Reactions in Chemical Systems[J].Appl Spectrosc,2015,69(11):1229-1242.
[9]张明发,沈雅琴.薏苡仁抗代谢综合征的药理作用研究进展[J].药物评价研究,2014,37(2):178-183.
[10]Chunqing Bai,Guowei Luo,Yuzhen Liu,et al.A Comparison Investigation of Coix Seed Oil Liposomes Prepared by Five Different Methods[J].Journal of Dispersion Science and Technology,2015,36(1),136-145.
[11]Ding Qu,Wenjie Sun,Mingjian Liu,et al.Bitargeted microemulsions based on coix seed ingredients for enhanced hepatic tumor delivery and synergistic therapy[J].International Journal of Pharmaceutics,2016,503(1-2):90-101.
[12]Xing Liu,Xin Zhang,Yu-Zhi Rong,et al.Rapid Determination of Fat,Protein and Amino Acid Content in Coix Seed Using Near-Infrared Spectroscopy Technique[J].Food Analytical Methods,2015,8(2):334-342.
[13]Liu Y,Zhang W,Wang XJ,et al.Antitumor effect of Kanglaite?injection in human pancreatic cancer xenografts[J].BMC Complement Altern Med,2014,14(3)228-234.
[14]Xiaoxiang Du,Huizhou Ye,Chunxia Zhang,et al.Effect of kanglaite on rat cytochrome P450[J].Pharmaceutical Biology,2014,53(7),995-1001.
[15]Julien Boccard,Serge Rudaz.Exploring Omics data from designed experiments using Analysis of Variance Multiblock Orthogonal Partial Least Squares[J].Analytica Chimica Acta,2016,920(12):18-28.