蛋白激酶SGK3过表达促进卵巢癌细胞SKOV3增殖和侵袭
摘要
目的研究血清和糖皮质激素诱导的蛋白激酶3(SGK3)过表达对卵巢癌细胞克隆增殖和侵袭行为的影响及其机制。方法免疫组化方法检测不同类型卵巢癌组织中SGK3蛋白的表达;通过Gen EscortTMⅠ介导用重组质粒p EGFPN1-SGK3瞬时转染卵巢癌SKOV3细胞,免疫印迹方法对转染细胞中的蛋白表达进行鉴定;克隆形成实验观察过表达SGK3后SKOV3细胞形成单个细胞克隆的能力;细胞侵袭实验用于观察在过表达SGK3后SKOV3细胞侵袭能力的变化;免疫印迹方法检测肿瘤转移相关基因的表达。结果 SGK3在卵巢癌组织的表达明显高于癌旁正常组织(P<0.05)。过表达SGK3的SKOV3细胞,其克隆形成和侵袭力均增强,与对照组细胞比较,差异有统计学意义(P<0.05)。结论 SGK3在卵巢癌组织中呈高表达,SGK3过表达可促进卵巢癌细胞的侵袭。
引文
[1]郭红艳,孙晓杰,李淑艳,等.蛋白激酶SGK3与乳腺癌临床病理相关性研究[J].现代预防医学,2016,43(6):1142-1145.
[2]胡明英,王晓莉,毕学辉,等.RNA干扰沉默AKT基因对卵巢癌SKOV3细胞增殖与凋亡影响研究[J].中华肿瘤防治杂志,2013,20(24):1869-1873.DOI:10.3969/j.issn.1673-5269.2013.24.002.
[3]刘斐,谭布珍.PI3K/AKT/GSK3β信号通路在上皮性卵巢癌细胞增殖与耐药中的作用[J].天津医药,2013,41(6):614-616.DOI:10.3969/j.issn.0253-9896.2013.06.033.
[4]De Marco C,Rinaldo N,Bruni P,et al.Multiple genetic alterations within the PI3K pathway are responsible for AKT activation in patients with ovarian carcinoma[J].PLo S One,2013,8(2):e55362.
[5]Hou M,Lai Y,He S,et al.SGK3(CISK)may induce tumor angiogenesis(Hypothesis)[J].Oncol Lett,2015,10(1):23-26.
[6]吕文杰,王洪涛,黄芳,等.过表达SGK3肝癌细胞BEL-7402在去甾体激素血清中的抗凋亡研究[J].生物技术通讯,2016,27(1):27-31.DOI:10.3969/j.issn.1009-0002.2016.01.006.
[7]郭红艳,孙晓杰,李淑艳,等.蛋白激酶SGK3基因的克隆及其在乳腺癌细胞中的表达[J].中国老年学杂志,2015,35(15):4187-4189.DOI:10.3969/j.issn.1005-9202.2015.15.034.
[8]Carico C,Nuno M,Mukheriee D,et al.Loss of PTEN is not associated with poor survival in newly diagnosed glioblastoma patients of the temozolomideera[J].Plos One,2012,7(3):e33684.
[9]Ferlay J,Shin HR,Bray F,et al.Estimates of worldwide burden of cancer in 2008:GLOBOCAN 2008[J].Int J Cancer,2010,127(12):2893-2917.
[10]Siegel R,Ward E,Brawley O,et al.Cancer statistics,2011:the impact of eliminating socioeconomic and racial disparities on premature cancer deaths[J].CA Cancer J Clin,2011,61(4):212-236.(收稿日期:)
[2]胡明英,王晓莉,毕学辉,等.RNA干扰沉默AKT基因对卵巢癌SKOV3细胞增殖与凋亡影响研究[J].中华肿瘤防治杂志,2013,20(24):1869-1873.DOI:10.3969/j.issn.1673-5269.2013.24.002.
[3]刘斐,谭布珍.PI3K/AKT/GSK3β信号通路在上皮性卵巢癌细胞增殖与耐药中的作用[J].天津医药,2013,41(6):614-616.DOI:10.3969/j.issn.0253-9896.2013.06.033.
[4]De Marco C,Rinaldo N,Bruni P,et al.Multiple genetic alterations within the PI3K pathway are responsible for AKT activation in patients with ovarian carcinoma[J].PLo S One,2013,8(2):e55362.
[5]Hou M,Lai Y,He S,et al.SGK3(CISK)may induce tumor angiogenesis(Hypothesis)[J].Oncol Lett,2015,10(1):23-26.
[6]吕文杰,王洪涛,黄芳,等.过表达SGK3肝癌细胞BEL-7402在去甾体激素血清中的抗凋亡研究[J].生物技术通讯,2016,27(1):27-31.DOI:10.3969/j.issn.1009-0002.2016.01.006.
[7]郭红艳,孙晓杰,李淑艳,等.蛋白激酶SGK3基因的克隆及其在乳腺癌细胞中的表达[J].中国老年学杂志,2015,35(15):4187-4189.DOI:10.3969/j.issn.1005-9202.2015.15.034.
[8]Carico C,Nuno M,Mukheriee D,et al.Loss of PTEN is not associated with poor survival in newly diagnosed glioblastoma patients of the temozolomideera[J].Plos One,2012,7(3):e33684.
[9]Ferlay J,Shin HR,Bray F,et al.Estimates of worldwide burden of cancer in 2008:GLOBOCAN 2008[J].Int J Cancer,2010,127(12):2893-2917.
[10]Siegel R,Ward E,Brawley O,et al.Cancer statistics,2011:the impact of eliminating socioeconomic and racial disparities on premature cancer deaths[J].CA Cancer J Clin,2011,61(4):212-236.(收稿日期:)