复方多花黄精混合提取物的降血脂作用
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摘要
目的:研究复方多花黄精混合提取物(FHT)对大鼠血脂的影响,为保健食品开发提供理论依据。方法:将65只SD雌性大鼠随机分成空白组,模型组,辛伐他汀阳性组(2 mg/kg),FHT高剂量(30 g生药/kg)、中剂量(15 g生药/kg)、低剂量组(7.5 g生药/kg)。空白组喂全价营养颗粒饲料,其余各组喂养高脂饲料并给予相应的药物灌胃,连续42 d。分别在第二周、第四周、第六周后,眼内眦采血,测定血清总胆固醇(TC)、血清甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)水平。结果:与模型组比较,FHT高剂量组在给药第二周后就能显著性降低TC和TG的水平(p <0.05),在给药第四周后能极显著地降低LDL-C水平和升高HDL-C水平(p <0.01); FHT中低量组在给药第四周后能显著改善TC、TG、LDL-C、HDL-C水平(p <0.05)。结论:FHT具有降血脂作用。
Objective: To study the effect of Polygonatum cyrtonema prescription extract on blood lipid in rats so as to provide basis for the development of health-care food. Methods: 65 female SD rats were randomly divided into control group,model group,positive group (simvastatin,2 mg/kg) and Polygonatum cyrtonema prescription extract groups (30,15,7.5 g/kg). The control group was fed granulated forage,the other groups were fed high fat diet and given the corresponding drug gavage for42 days. The levels of serum total cholesterol (TC),serum triglyceride (TG),low density lipoprotein (LDL-C),high density lipoprotein (HDL) were measured at the 2 nd,4 th and 6 th week after the blood was drawn from inner canthus.Results: Compared with the model group,the high dose group of Polygonatum cyrtonema prescription extract could significantly decrease the level of TC and TG after 2 weeks (p < 0.05),significantly decreased LDL-C level and elevated HDL-C level after 4 weeks (p <0.01).The level of TC,TG,LDL-C and HDL-C also improved significantly by middle and low dose groups of Polygonatum cyrtonema prescription extract after 4 weeks (p < 0.05). Conclusion: Polygonatum cyrtonema prescription extract had hypolipidemic effect.
Objective: To study the effect of Polygonatum cyrtonema prescription extract on blood lipid in rats so as to provide basis for the development of health-care food. Methods: 65 female SD rats were randomly divided into control group,model group,positive group (simvastatin,2 mg/kg) and Polygonatum cyrtonema prescription extract groups (30,15,7.5 g/kg). The control group was fed granulated forage,the other groups were fed high fat diet and given the corresponding drug gavage for42 days. The levels of serum total cholesterol (TC),serum triglyceride (TG),low density lipoprotein (LDL-C),high density lipoprotein (HDL) were measured at the 2 nd,4 th and 6 th week after the blood was drawn from inner canthus.Results: Compared with the model group,the high dose group of Polygonatum cyrtonema prescription extract could significantly decrease the level of TC and TG after 2 weeks (p < 0.05),significantly decreased LDL-C level and elevated HDL-C level after 4 weeks (p <0.01).The level of TC,TG,LDL-C and HDL-C also improved significantly by middle and low dose groups of Polygonatum cyrtonema prescription extract after 4 weeks (p < 0.05). Conclusion: Polygonatum cyrtonema prescription extract had hypolipidemic effect.
引文
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[12]王涛涛,程娟,姚余有.黄精水煎剂对β-淀粉样蛋白诱导的大鼠学习记忆能力下降的保护作用研究[J].安徽农业大学学报,2013,40(1):95-99.
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[14]范婷婷,法鲁克,方芳,等.荷叶总生物碱降脂减肥作用的体内外试验[J].浙江大学学报:农业与生命科学版,2013,39(2):141-148.
[15]黎海彬,方昆阳,吕翠婷,等.决明子、山楂提取物不同配比降血脂作用的研究[J].中药材,2007,5(30):573-575.
[2]陈雅利.饮食干预对高脂血症患者血脂水平的影响[J].当代护士(综合版),2008(7):36-37.
[3]国家药典委员会.中华人民共和国药典.一部[S].北京:中国医药科技出版社,2015:1121.
[4]王蔚倩,王思玉,沈以丹,等.复方青钱柳茶对高脂血症模型大鼠血脂的影响[J].临床医药文献电子杂志,2017,4(21):3973-3974.
[5]王伟.精制山荷降脂方提取工艺及药效学的初步研究[D].广州:第一军军医大学,2004.
[6]罗敏,章文伟,邓才富,等.药用植物多花黄精研究进展[J].时珍国医国药,2016,27(6):1467-1469.
[7]Wang S Y,Yu Q J,Bao J K,et al.Polygonatum cyrtonema lectin,a potential antineoplastic drug targeting programmed cel death pathways[J].Biochemical&Biophysical Research Communications,2011,406(4):497-500.
[8]Liu F,Liu Y,Meng Y,et al.Structure of polysaccharide from Polygonatum cyrtonema Hua and the antiherpetic activity of its hydrolyzed fragments[J].Antiviral Research,2004,63(3):183-189.
[9]Ding J,Bao J,Zhu D,et al.Crystal structures of a novel antiHIV mannose-binding lectin from Polygonatum cyrtonema Hua with unique ligand-binding property and super-structure[J].Journal of Structural Biology,2010,171(3):309.
[10]An J,Liu J Z,Wu C F,et al.Anti-HIV I/II activity and molecular cloning of a novel mannose/sialic acid-binding lectin from rhizome of Polygonatum cyrtonema Hua[J].Acta Biochimica Et Biophysica Sinica,2006,38(2):70-78.
[11]郑春艳,汪好芬,张庭廷.黄精多糖的抑菌和抗炎作用研究[J].安徽师范大学学报:自然科学版,2010,33(3):272-275.
[12]王涛涛,程娟,姚余有.黄精水煎剂对β-淀粉样蛋白诱导的大鼠学习记忆能力下降的保护作用研究[J].安徽农业大学学报,2013,40(1):95-99.
[13]潘琼.枸杞黄精组方口服液制备和药理作用研究[D].银川:宁夏医科大学,2014.
[14]范婷婷,法鲁克,方芳,等.荷叶总生物碱降脂减肥作用的体内外试验[J].浙江大学学报:农业与生命科学版,2013,39(2):141-148.
[15]黎海彬,方昆阳,吕翠婷,等.决明子、山楂提取物不同配比降血脂作用的研究[J].中药材,2007,5(30):573-575.