西藏拉萨市鸡源沙门氏菌分子生物学鉴定与血清分型
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摘要
鸡源沙门氏菌作为重要的食源性病原菌,其血清型分布和致病性研究对于维护公共卫生安全具有重要意义。本研究分别在西藏拉萨市养鸡密集的城关区、曲水县、达孜县、墨竹工卡县、堆龙德庆县5个地区,选择12个养鸡场,采集疑似发病鸡样品121份,最终分离鉴定出104株沙门氏菌。血清学鉴定结果显示,这104株沙门氏菌分属于4个血清群,其中A群(O2)13株、C1群(O7)29株、C2群(O8)9株、D群(O9)53株;优势血清群为D群(51%),其次为C1群(27%)。PCR扩增结果显示,这104株沙门氏菌和标准阳性对照均扩增出inv A基因片断,表明均为致病性沙门氏菌。动物致病性试验结果显示,7日龄雏鸡接种后感染率可达100%。上述结果表明,拉萨市鸡源沙门氏菌流行较为普遍,且均有致病性,会对当地禽产品卫生安全带来威胁,因此西藏地区需要加强鸡源沙门氏菌的控制。
As an important food-borne pathogen,chicken Salmonella seriously affects the public health security,therefore,the study on its serotype distribution and pathogenicity is of great significance. In this study,121 samples from suspected chickens were collected from 12 farms in Chengguan District,Qushui County,Dazi County,Maizhokunggar County and Duilongdeqing County,and 104 strains of Salmonella were isolated. Serological tests showed that the 104 strains of Salmonella belonged to 5 serum groups,in which 13 were from A serogroup(O2),29 from C1(O7),9 from C2(O8),53 from D(O9) and 9 from C2(O8),and the dominant were from serogroup D(51%),followed by C1(27%). The invA gene fragments of 104 Salmonella strains and standard positive control were amplified by PCR,indicating that they were all pathogenic Salmonella. Animal pathogenicity test results showed that the infection rate of 7-day-old chickens could reach to 100%. In conclusion,the chicken-sourced pathogenic Salmonella in Lhasa City was wide spread,posing threat to hygiene and security of local poultry products. Therefore,the control over chicken-sourced Salmonella should be intensified.
As an important food-borne pathogen,chicken Salmonella seriously affects the public health security,therefore,the study on its serotype distribution and pathogenicity is of great significance. In this study,121 samples from suspected chickens were collected from 12 farms in Chengguan District,Qushui County,Dazi County,Maizhokunggar County and Duilongdeqing County,and 104 strains of Salmonella were isolated. Serological tests showed that the 104 strains of Salmonella belonged to 5 serum groups,in which 13 were from A serogroup(O2),29 from C1(O7),9 from C2(O8),53 from D(O9) and 9 from C2(O8),and the dominant were from serogroup D(51%),followed by C1(27%). The invA gene fragments of 104 Salmonella strains and standard positive control were amplified by PCR,indicating that they were all pathogenic Salmonella. Animal pathogenicity test results showed that the infection rate of 7-day-old chickens could reach to 100%. In conclusion,the chicken-sourced pathogenic Salmonella in Lhasa City was wide spread,posing threat to hygiene and security of local poultry products. Therefore,the control over chicken-sourced Salmonella should be intensified.
引文
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[13]孔繁德.沙门氏菌快速检测体系的建立与应用及耐药性分析研究[D].南京:南京农业大学,2006:63-70.
[14]周廷宣.后备种鸡血清中沙门氏菌病抗体的检测[J].中国畜牧兽医,2007(34):87.
[15]张秀珍.当代细菌检验与临床[M].北京:人民卫生出版社,2000.
[2]李可,方莹,张晓峰,等.沙门氏菌的血清分型及分子鉴定研究进展[J].食品安全质量检测学报,2016,7(10):3947-3951.
[3]赵建梅,李月华,宋传周,等. PCR鉴定沙门氏菌血清分型方法的建立与应用[J].中国动物检疫,2018,35(1):21-25.
[4]陆承平.兽医微生物学[M]. 3版.北京:中国农业出版社,2001.
[5]农业部.动物和动物产品沙门氏菌检测方法:NY/T550—2002[S].北京:农业部,2002.
[6]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001.
[7]布坎南R E,吉本斯N E.伯杰细菌鉴定手册[M]. 8版.中国科学院微生物研究所,译.北京:科学出版社,1984:392-442.
[8]姚火春.兽医微生物学实验指导[M].北京:中国农业出版社,2002.
[9]马兴树.禽传染病实验诊断技术[M].北京:化学工业出版社,2006:106-112.
[10]赵连生.鸡源沙门氏菌的分离鉴定、耐药性检测及耐药基因定位[D].重庆:西南大学,2009.
[11]胡婧,朱战波,申欣,等.大庆某牛场牛舍环境中沙门氏菌的调查与分析[J].中国畜牧杂志,2013(8):20-23.
[12]顾国强,杜泳铭,史岩.种鸡场鸡白痢净化综合措施[J].中国禽业导刊,2001(10):30.
[13]孔繁德.沙门氏菌快速检测体系的建立与应用及耐药性分析研究[D].南京:南京农业大学,2006:63-70.
[14]周廷宣.后备种鸡血清中沙门氏菌病抗体的检测[J].中国畜牧兽医,2007(34):87.
[15]张秀珍.当代细菌检验与临床[M].北京:人民卫生出版社,2000.