贵州省盘县家蝇抗药性调查及防制策略分析
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摘要
目的了解贵州省盘县家蝇对常用杀虫剂的抗药性状况,为合理用药提供依据。方法 2016年6—7月,采集贵州省盘县不同方位的家蝇,采用微量点滴法测定现场种群成蝇对高效氯氰菊酯、敌敌畏和残杀威的抗药性,利用SPSS 25.0统计软件计算半数致死量(LD_(50))、95%CI,并将野外种群与敏感种群进行比较得出抗性倍数。结果 2016年贵州省盘县家蝇对高效氯氰菊酯、敌敌畏和残杀威的半数致死量(LD_(50))分别为1.3758、0.6615、48.4511μg/♀,抗性倍数分别为443.81、62.41和141.22,抗性程度从高到低依次为高效氯氰菊酯、残杀威和敌敌畏。结论贵州省盘县野外家蝇对3种卫生杀虫剂产生了不同程度的抗药性,个别药剂如高效氯氰菊酯已基本无效。贵州省盘县蝇类控制工作应进一步注重可持续控制策略,以环境治理与物理防制为主要控制手段,必要时选择抗性低的卫生杀虫剂,科学合理施药,以控制或延缓家蝇抗药性的产生和发展。
Objective To investigate the susceptibility of Musca domestica to commonly used insecticides,and to provide a scientific basis for rational use of insecticides.Methods Musca domesticawere collected from Pan County from June to July, 2016. Topical application was used to determine the resistance of M. domestica to beta-cypermethrin, dichlorvos,propoxur, and data were analyzed by SPSS 25.0 software for LD_(50) and its confidential intervals(CI), obtaining resistance ratio by comparing with susceptible strain.Results The LD_(50) values of Musca domestica to beta-cypermethrin, dichlorvos and propoxur were 1.3758,0.6615,and 48.4511 μg/♀, and the resistance ratio were 443.81, 62.41, and 141.22 folds. The order of insecticide resistance from high to low, was beta-cypermethrin, propoxur, and dichlorvos.Conclusion Field collected strains of Musca domestica in Pan County have already developed different resistance to three kinds of insecticides. Some insecticides, such as beta-cypermethrin, have been ineffective. Sustainable integrated response strategies should be established in M. domestica control in Pan County of Guizhou. In order to prevent and delay resistance, environmental control and physical control should be considered as the main control means; if necessary, chemical insecticides with low resistance can be selected based on the resistance monitoring data.
Objective To investigate the susceptibility of Musca domestica to commonly used insecticides,and to provide a scientific basis for rational use of insecticides.Methods Musca domesticawere collected from Pan County from June to July, 2016. Topical application was used to determine the resistance of M. domestica to beta-cypermethrin, dichlorvos,propoxur, and data were analyzed by SPSS 25.0 software for LD_(50) and its confidential intervals(CI), obtaining resistance ratio by comparing with susceptible strain.Results The LD_(50) values of Musca domestica to beta-cypermethrin, dichlorvos and propoxur were 1.3758,0.6615,and 48.4511 μg/♀, and the resistance ratio were 443.81, 62.41, and 141.22 folds. The order of insecticide resistance from high to low, was beta-cypermethrin, propoxur, and dichlorvos.Conclusion Field collected strains of Musca domestica in Pan County have already developed different resistance to three kinds of insecticides. Some insecticides, such as beta-cypermethrin, have been ineffective. Sustainable integrated response strategies should be established in M. domestica control in Pan County of Guizhou. In order to prevent and delay resistance, environmental control and physical control should be considered as the main control means; if necessary, chemical insecticides with low resistance can be selected based on the resistance monitoring data.
引文
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[2]赵杨,许明,王伟娜,等.天津市东丽区家蝇对4种杀虫剂抗药性趋势调查研究[J].中国媒介生物学及控制杂志,2018,29(5):521-522,529.
[3]侯娟,王金娜,郭颂,等.浙江省2017年家蝇抗药性监测结果分析[J].中国媒介生物学及控制杂志,2018,29(4):348-350.
[4]韩坤,张术斌,杨聪,等.宁夏部分地区家蝇对5种卫生杀虫剂的抗药性调查[J].中国媒介生物学及控制杂志,2017,28(5):499-501.
[5]缪金华,陈学良,马桢红,等.昆山市蚊蝇抗药性调查[J].中国热带医学,2006,6(4):744.
[6]中国国家标准化管理委员会.蝇类抗药性检测方法家蝇生物测定法:GB/T26350-2010[S].北京:中国标准出版社,2011.
[7]吴志凤,王以燕,顾宝根.我国卫生杀虫剂的现状与发展前景[J].农药科学与管理,2011,32(5):16-19.
[8]梁文琴,田珍灶,杨迅,等.兴义市家蝇抗药性调查研究[J].中华卫生杀虫药械,2016,22(4):349-350.
[9]马卓,周小洁,李静,等.北京地区2017年家蝇种群对常用杀虫药剂的抗药性调查[J].中国媒介生物学及控制杂志,2019,30(1):63-65.
[10]韦凌娅,孔庆鑫,陈冰冰,等.杭州市家蝇对常用卫生杀虫剂的抗药性研究[J].中国媒介生物学及控制杂志,2017,28(5):451-453.
[11]王恺,林在生,卢翠英,等.福建省2011-2015年农村环境卫生健康危害因素调查[J].中国热带医学,2018,18(11):1114-1119.