血硒水平及GPX3表达水平与甲状腺癌相关性研究
摘要
目的探讨血硒水平与GPX3表达水平与甲状腺癌、甲状腺良性病变的相关性。方法选择甲状腺癌组织标本110例,甲状腺腺瘤组织标本35例,结节性甲状腺肿组织标本46例,正常甲状腺组织标本20例。采用免疫组化方法测定四组中GPX3的表达水平。同时测定血清中硒水平浓度。收集正常对照组(无甲状腺疾病的健康人群)血清标本,测定其血清硒浓度。结果 110例甲状腺癌组织中GPX3蛋白的阳性表达率是39.10%(43/110),呈低表达;35例甲状腺腺瘤组织中GPX3蛋白的阳性表达率是77.14%(27/35),呈中高等表达;46例结节性甲状腺肿组织中GPX3蛋白的阳性表达率是78.26%(36/46),呈中高等表达;20例正常甲状腺组织中GPX3蛋白的阳性表达率是80.00%(16/20),呈现高表达。实验结果显示同甲状腺正常组织及良性病变组织相比,甲状腺癌组织中GPX3表达情况明显偏低,χ~2=16.381,P<0.05,差异具有统计学意义。甲状腺癌组血硒水平(86.58±10.57)ug/L,非甲状腺癌组血硒水平(109.81±54.43)ug/L,正常对照组血硒水平(128.67±35.75)ug/L。甲状腺癌组血硒水平明显低于非甲状腺癌组以及正常对照组,差异具有统计学意义。结论检测血硒及GPX3蛋白的表达水平不仅为甲状腺癌的早期诊断提供了可靠参考数据,并且为临床治疗提供可靠依据。
引文
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[3]Kucharzewski M,Braziewicz J,Majewska U,et al.Concentration of selenium in the whole blood and the thyroid tissue of patients with various thyroid diseases[J].Biol Trace Elem Res,2012,88(1):25-30.
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[5]Iwasaki Y.Disorders in thyroid hormone metabolism[J].Rinsho Byori,2013,58(3):238-243.
[6]Erdamar H,Cimen B,Gülcemal H,etal.Increased lipid peroxidation and impaired enzymatic antioxidant defense mechanism in thyroid tissue with multinodular goiter and papillary carcinoma[J].Clin Biochem,2012,43(7-8):650-654.