食管鳞癌中肝细胞生长因子、血管内皮生长因子C及其受体3的表达及意义
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摘要
目的:探讨肝细胞生长因子(HGF)、血管内皮细胞生长因子C(VEGF-C)和其受体3(VEGFR-3)在食管鳞癌中的表达水平及与食管鳞癌临床病理因素之间的关系。方法:采用RT-PCR技术检测25例食管正常黏膜、60例食管鳞癌组织中HGF、VEGF-C、VEGFR-3 mRNA的表达水平,并应用免疫组织化学法检测HGF、VEGF-C、VEGFR-3蛋白的表达水平并分析其与食管鳞癌临床病理因素之间的关系。结果:食管鳞癌组织中HGF、VEGF-C、VEGFR-3表达量明显高于食管正常黏膜组织,并且HGF、VEGF-C、VEGFR-3与食管鳞癌淋巴结转移、TNM分期呈正相关。结论:HGF、VEGF-C、VEGFR-3在食管鳞癌的发生发展中起到重要作用,三者可能协同作用在食管癌浸润生长、淋巴结转移及远处播散过程中起到一定作用。
Objective: To investigate the role of HGF,VEGF-C,VEGFR-3 on the occurrence and development of human esophageal squamous cell carcinoma. Methods: RT-PCR technique was used to examine expression of HGF,VEGF-C and VEGFR-3 mRNA and immunohistochemical staining was used to examine expression of HGF,VEGF-C and VEGFR-3 in 25 cases of normal mucosa and 60 cases of ESCC tissues. Results: Expression of HGF,VEGF-C and VEGFR-3 in ESCC showed significantly higher than those in normal mucosa( P < 0. 01,P < 0. 01,P< 0. 01),and they were positively correlated with lymph node metastasis and TNM stage of ESCC. Conclusion: The high expression of HGF,VEGF-C and VEGFR-3 may play an important role in the occurrence and development of ESCC. HGF,VEGF-C and VEGFR-3 may interact with each other to promote the generation and development of ESCC.
Objective: To investigate the role of HGF,VEGF-C,VEGFR-3 on the occurrence and development of human esophageal squamous cell carcinoma. Methods: RT-PCR technique was used to examine expression of HGF,VEGF-C and VEGFR-3 mRNA and immunohistochemical staining was used to examine expression of HGF,VEGF-C and VEGFR-3 in 25 cases of normal mucosa and 60 cases of ESCC tissues. Results: Expression of HGF,VEGF-C and VEGFR-3 in ESCC showed significantly higher than those in normal mucosa( P < 0. 01,P < 0. 01,P< 0. 01),and they were positively correlated with lymph node metastasis and TNM stage of ESCC. Conclusion: The high expression of HGF,VEGF-C and VEGFR-3 may play an important role in the occurrence and development of ESCC. HGF,VEGF-C and VEGFR-3 may interact with each other to promote the generation and development of ESCC.
引文
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[5]Wakisaka N,Hirota K,Kondo S,et al.Induction of lymphangiogenesis through vascular endothelial growth factor-C/vascular endothelial growth factor receptor 3 axis and its correlation with lymph node metastasis in nasopharyngeal carcinoma[J].Oral Oncol,2012,48(8):703-708.
[6]Zhang XY,Zhang YM,Yang CH,et al.Expression of HGF,c-met and VEGF-C in cervical carcinoma[J].Cancer Research on Prevention and Treatment,2011,38(4):411-415.[张雪玉,张咏梅,杨彩虹,等.HGF、c-met和VEGF-C在宫颈癌中的表达[J].肿瘤防治研究,2011,38(4):411-415.]
[7]Saito S,Morishima K,Ui T,et al.The role of HGF/MET and FGF/FGFR in fibroblast derived growth stimulation and lapatinib-resistance of esophageal squamous cell carcinoma[J].BMC Cancer,2015,15:82.
[8]Ozawa Y,Nakamura Y,Fujishima F,et al.c-Met in esophageal squamous cell carcinoma:An independent prognostic factor and potential therapeutic target[J].BMC Cancer,2015,15:451.
[9]Ren Y,Cao B,Law S,et al.Hepatocyte growth factor promotes cancer cell migration and angiogenic factors expression:A prognostic maker of human esophageal squamous cell carcinomas[J].Clin Cancer Res,2005,11(17):6190-6197.
[10]Yuan HQ,Kong F,Wang XL,et al.Inhibitory effect of acetyl-11-keto-beta-boswellic acid on androgen receptor by interference of Sp1 binding activity in prostate cancer cells[J].Biochemical Pharmacology,2008,75:2112-2121.
[11]He Hailang,Zhou Xianmei.VEGF-C/VEGFR-3 and SDF-1/CXCR4 signaling pathways in cancer lymphangiogenesis[J].Modern Oncology,2014,22(8):1958-1962.[何海浪,周贤梅.VEGF-C/VEGFR-3及SDF-1/CXCR4信号途径与肿瘤淋巴管新生的关系[J].现代肿瘤医学,2014,22(8):1958-1962.]
[12]Long Qiongxian,Tang Zhirong,Wu Cailiang,et al.The correlation and significance of VEGF-C and Trk B protein expression in epithelial ovarian carcinoma[J].Modern Oncology,2016,24(16):2579-2583.[龙琼先,唐治蓉,吴才良,等.卵巢上皮性癌中VEGF-C和Trk B蛋白表达的相关性及意义[J].现代肿瘤医学,2016,24(16):2579-2583.]
[13]Xu ZB,Feng JB,Yuan L,et al.Changes in levels of HGF,c-met and VEGF expression in esophageal carcinogenesis[J].Shandong Medical Journal,2016,56(1):9-11.[徐志彬,冯军波,袁丽,等.食管癌变进程中HGF、c-met、VEGF蛋白的表达变化及意义[J].山东医药,2016,56(1):9-11.]