八珍汤对荷结肠癌小鼠皮下移植瘤中CXCR4和CD133共表达的影响
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摘要
目的观察八珍汤对结肠癌皮下移植瘤模型小鼠癌组织中CXCR4及CD133共表达的影响,探讨八珍汤抑制结肠癌移植瘤生长的机制。方法将70只裸鼠随机分为模型组35只、八珍汤组35只。2组均采用结肠癌CT26细胞混悬液直接注射的方法建立皮下移植瘤模型,造模后第10天起,模型组以无菌蒸馏水灌胃,八珍汤组以八珍汤灌胃,均连续灌胃10 d。自灌胃开始后第2,4,6,8,10天测量肿瘤大小并计算体积,然后计算抑瘤率。灌胃后的第11天处死2组裸鼠,取瘤组织,常规HE染色观察,并采用免疫组化法检测瘤组织中CXCR4和CD133表达情况。结果灌胃后第2,4,6,8,10天,八珍汤组肿瘤体积均明显小于模型组(P均<0.05),八珍汤组抑瘤率为56.13%。移植瘤组织HE染色显示,八珍汤组较模型组细胞异型性小,且细胞排列成团现象不明显。2组CXCR4、CD133蛋白阳性表达率比较虽然差异均无统计学意义(P均>0.05),但八珍汤组约较模型组降低10%。结论八珍汤可以通过抑制CXCR4及CD133的共表达来抑制肿瘤的生长。
Objective It is to observe the effect of Bazhen Decoction on the co-expression of CXCR4 and CD133 in mouse cancer tissues of colon cancer subcutaneous xenograft model, and to explore the mechanism of Bazhen Decoction in inhibiting the growth of colon cancer xenografts. Methods 70 nude mice were randomly divided into model group(n=35) and Bazhen decoction group(n=35). The subcutaneous xenograft model was established by direct injection of colon cancer CT26 cell suspension in the two groups. From the 10 th day after model establishment, the model group was perfused with sterile distilled water, and the Bazhen Decoction group was administered with Bazhen Decoction, both groups were treated continuously by gavage for 10 d. The tumor size was measured and volume was calculated on days 2, 4, 6, 8, and 10 after the start of gavage, and then the tumor inhibition rate was calculated. On the 11 th day after intragastric administration, the mude mice in the two groups were sacrificed. The tumor tissues were taken and observed by routine HE staining. The expression of CXCR4 and CD133 in tumor tissues was detected by immunohistochemistry. Results On the 2 nd, 4 th, 6 th, 8 th and 10 th day after intragastric administration, the tumor volume of Bazhen Decoction group was significantly lower than that of the model group(P<0.05), and the inhibition rate of Bazhen Decoction group was 56.13%. HE staining of transplanted tumor tissue showed that the Bazhen decoction group had smaller cell type than the model group, and the cell arrangement was not obvious. There was no significant difference in the positive expression rates of CXCR4 and CD133 between the two groups(P>0.05), but the Bazheng group was reduced by 10% compared with the model group. Conclusion Bazhen Decoction can inhibit tumor growth by inhibiting the co-expression of CXCR4 and CD133.
Objective It is to observe the effect of Bazhen Decoction on the co-expression of CXCR4 and CD133 in mouse cancer tissues of colon cancer subcutaneous xenograft model, and to explore the mechanism of Bazhen Decoction in inhibiting the growth of colon cancer xenografts. Methods 70 nude mice were randomly divided into model group(n=35) and Bazhen decoction group(n=35). The subcutaneous xenograft model was established by direct injection of colon cancer CT26 cell suspension in the two groups. From the 10 th day after model establishment, the model group was perfused with sterile distilled water, and the Bazhen Decoction group was administered with Bazhen Decoction, both groups were treated continuously by gavage for 10 d. The tumor size was measured and volume was calculated on days 2, 4, 6, 8, and 10 after the start of gavage, and then the tumor inhibition rate was calculated. On the 11 th day after intragastric administration, the mude mice in the two groups were sacrificed. The tumor tissues were taken and observed by routine HE staining. The expression of CXCR4 and CD133 in tumor tissues was detected by immunohistochemistry. Results On the 2 nd, 4 th, 6 th, 8 th and 10 th day after intragastric administration, the tumor volume of Bazhen Decoction group was significantly lower than that of the model group(P<0.05), and the inhibition rate of Bazhen Decoction group was 56.13%. HE staining of transplanted tumor tissue showed that the Bazhen decoction group had smaller cell type than the model group, and the cell arrangement was not obvious. There was no significant difference in the positive expression rates of CXCR4 and CD133 between the two groups(P>0.05), but the Bazheng group was reduced by 10% compared with the model group. Conclusion Bazhen Decoction can inhibit tumor growth by inhibiting the co-expression of CXCR4 and CD133.
引文
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[2] 王红鲜.慢病毒载体介导的CXCR7shRNA对结肠癌生物学行为的影响[D].长沙:中南大学,2009
[3] 刘鲲,刘娜,刘苾川,等.人参抗肿瘤药理研究[J].光明中医,2016,31(1):140-143
[4] 王清,刘倩,张学梅.P-糖蛋白及其逆转肿瘤细胞多药耐药性的研究进展[J].中国执业药师,2013,10(3):33-36
[5] 王君明,郭晓娜,张月月,等.地黄多糖和寡糖的药理作用研究进展[J].中国老年学杂志,2015,35(22):6603-6605
[6] 苗明三,方晓艳.怀地黄多糖免疫兴奋作用的实验研究[J].中国中医药科技,2002,9(3):159-160
[7] 李乃谦.探讨白芍的药理作用及现代研究进展[J].中医临床研究,2017,9(20):137-138
[8] 周强,栗占国.白芍总苷的药理作用及其在自身免疫性疾病中的应用[J].中国新药与临床杂志,2003,22(11):687-691
[9] 金剑,赵庆.川芎素对结直肠癌Lovo细胞生长的体内外抑制作用及机制[J].山东医药,2016,56(34):8-10
[10] 周立,张玮.茯苓素诱生肿瘤坏死因子的作用[J].中国抗生素杂志,1994,19(5):376-380
[11] 程尧,王凯平,李强,等.当归多糖对H22荷瘤小鼠肿瘤生长及体内铁代谢的影响[J].中国医院药学杂志,2015,35(15):1359-1363
[12] 陆家佳.白术挥发性成分GC-MS分析及对五种肿瘤细胞抑制活性研究[J].海峡药学,2016,28(6):28-31
[13] 熊蕴珠.白术内酯Ⅰ对MyD88+人卵巢癌细胞分泌的免疫抑制因子TGF-β1、IL-17A、IL-10和IL-4的影响[D].成都:成都中医药大学,2014
[14] 王苗苗.白术内酯I拮抗紫杉醇诱导卵巢癌细胞分泌IL-6和化疗耐受[D].成都:成都中医药大学,2010
[15] 陈福,许厚强,段志强,等.甘草次酸调节BLM的转录及PC3细胞的增殖凋亡和迁移侵袭[J].生物技术,2017,27(3):282-288
[16] 沈云,李继坤.CXCL12-CXCR4生物学轴在结直肠癌中的研究进展[J].中国癌症杂志,2008,18(3):237-240
[17] 方砚田.结肠癌干细胞分选、差异microRNA表达谱检测以及miR-449b-CCND1、E2F3通路在结肠癌干细胞自我更新的机制研究[D].上海:复旦大学,2014
[18] Squatrito M,Holland EC,Hambardzumyan D.Radiation resistance and stem-like cells in brain tumors[J].Cancer Cell,2006,10(6):454-456
[19] Zhang NH,Li J,Li Y,et al.Co-expression of CXCR4 and CD133 proteins is associated with poor prognosis in stage II-III colon cancer patients[J].Exp Ther Med,2012,3(6):973-982
[20] 徐可.CD133+ CXCR4+结直肠癌肿瘤细胞的生物学特性及对结直肠癌肝转移的影响[D].福州:福建医科大学,2013