间日疟原虫感染患者T细胞应答相关趋化因子水平的检测
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摘要
T细胞介导的细胞免疫应答和体液免疫应答对控制疟原虫红内期感染至关重要。免疫细胞的募集和功能调节受到不同趋化因子的调控。进一步寻找招募T细胞的关键性趋化因子将为针对性调控抗疟免疫应答类型提供有效靶点。通过收集并检测中缅边境地区间日疟原虫感染患者血浆中T细胞应答相关的趋化因子水平,并比较CD4~+T细胞和CD8~+T细胞不同表达水平患者的相应趋化因子表达,发现CXCL9、CXCL10、CXCL11、CXCL13、CCL13和CCL1在间日疟急性感染期均显著升高,CXCL10和CXCL13水平与患者的年龄呈正相关,特别是CXCL10对CD4~+T细胞的募集调控可能起到关键性作用。
T cell-mediated cellular and humoral immune responses are the most critical for controlling erythrocytic Plasmodium infection. The recruitment and functional regulation of immune cells are laid under regulation of different chemokines. Further pursuing key chemokines responsible for recruiting T cells will provide effective targets to modify the regulation aiming at anti-malaria immune response types. In this paper, the levels of chemokines related with T cell responses in patients′ plasma infected with tertian plasmodium(P. vivax) along China-Myanmar border were collected, detected, and compared the expressions of related chemokines in patients′ different expression levels of CD4~+T cells and CD8~+T cells, and found that CXCL9, CXCL10, CXCL11, CXCL13, CCL13 and CCL1 significantly increased in the acute infection period of P. vivax, levels of CXCL10 and CXCL13 assumed positive relativity with patients′ age, especially CXCL10 might play a key role in the CD4~+ T cell recruitment regulation.
T cell-mediated cellular and humoral immune responses are the most critical for controlling erythrocytic Plasmodium infection. The recruitment and functional regulation of immune cells are laid under regulation of different chemokines. Further pursuing key chemokines responsible for recruiting T cells will provide effective targets to modify the regulation aiming at anti-malaria immune response types. In this paper, the levels of chemokines related with T cell responses in patients′ plasma infected with tertian plasmodium(P. vivax) along China-Myanmar border were collected, detected, and compared the expressions of related chemokines in patients′ different expression levels of CD4~+T cells and CD8~+T cells, and found that CXCL9, CXCL10, CXCL11, CXCL13, CCL13 and CCL1 significantly increased in the acute infection period of P. vivax, levels of CXCL10 and CXCL13 assumed positive relativity with patients′ age, especially CXCL10 might play a key role in the CD4~+ T cell recruitment regulation.
引文
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[2] Dayananda KK,Achur RN,Gowda DC.Epidemiology,drug resistance,and pathophysiology of Plasmodium vivax malaria[J].J Vector Borne Dis,2018,55(1):1-8.
[3] WHO.Worldreport2017[EB/OL].http://www.who.int/malaria/publications/world-malaria-report-2017/en/.
[4] Lyke KE,Ishizuka AS,Berry AA,et al.Attenuated PfSPZ Vaccine induces strain-transcending T cells and durable protection against heterologous controlled human malaria infection[J].Proc Natl Acad Sci U S A,2017,114(10):2711-2716.
[5] Kimura K,Kimura D,Matsushima Y,et al.CD8+T cells specific for a malaria cytoplasmic antigen form clusters around infected hepatocytes and are protective at the liver stage of infection[J].Infect Immun,2013,81(10):3825-3834.
[6] 吕衍民,郁春云,刘飞,等.TLR4激活剂对约氏疟原虫感染早期免疫应答的影响[J].微生物学杂志,2016,36(5):57-61.
[7] 冯永辉,朱晓彤,单风平,等.大蒜素对致死型约氏疟原虫鼠疟模型CD4+活化/凋亡T细胞的影响[J].微生物学杂志,2016,36(3):59-64.
[8] 冯辉,潘艳艳,李莹,等.CD4+CD25+Foxp3hi细胞在夏氏疟原虫感染DBA/2小鼠作用研究[J].微生物学杂志,2012,32(2):41-46.
[9] 郑丽,孙晓丹,潘艳艳,等.BALB/c小鼠感染不同疟原虫CD4+Th应答和凋亡特点的比较研究[J].微生物学杂志,2011,31(1):19-23.
[10] Campanella GS,Tager AM,El Khoury JK,et al.Chemokine receptor CXCR3 and its ligands CXCL9 and CXCL10 are required for the development of murine cerebral malaria[J].Proc Natl Acad Sci USA,2008,105(12):4814-4819.
[11] Zhao Y,Zeng J,Zhao Y,et al.Risk factors for asymptomatic malaria infections from seasonal cross-sectional surveys along the China-Myanmar border[J].Malar J,2018,17(1):247.
[12] Zhao Y,Zhao Y,Lv Y,et al.Comparison of methods for detecting asymptomatic malaria infections in the China-Myanmar border area[J].Malar J,2017,16(1):159.
[13] Ourives SS,Borges QI,Dos Santos DSA,et al.Analysis of the lymphocyte cell population during malaria caused by Plasmodium vivax and its correlation with parasitaemia and thrombocytopaenia[J].Malar J,2018,17(1):303.
[14] Junqueira C,Barbosa CRR,Costa PAC,et al.Cytotoxic CD8+ T cells recognize and kill Plasmodium vivax-infected reticulocytes[J].Nat Med,2018,24(9):1330-1336.
[15] Wang QH,Liu YJ,Liu J,et al.Plasmodium yoelii:assessment of production and role of nitric oxide during the early stages of infection in susceptible and resistant mice[J].Exp Parasitol,2009,121(3):268-273.
[16] Sampaio NG,Eriksson EM,Schofield L.Plasmodium falciparum PfEMP1 Modulates Monocyte/Macrophage Transcription Factor Activation and Cytokine and Chemokine Responses[J].Infect Immun,2018,86:e00447-17.
[17] Cruz LAB,Barral-Netto M,Andrade BB.Distinct inflammatory profile underlies pathological increases in creatinine levels associated with Plasmodium vivax malaria clinical severity[J].PLoS Negl Trop Dis,2018,12(3):e0006306.
[18] Griffith JW,Sokol CL,Luster AD.Chemokines and chemokine receptors:positioning cells for host defense and immunity[J].Annu Rev Immunol,2014,32:659-702.
[19] Koper OM,Kamińska J,Sawicki K,et al.CXCL9,CXCL10,CXCL11,and their receptor (CXCR3) in neuroinflammation and neurodegeneration[J].Adv Clin Exp Med,2018,27(6):849-856.
[20] Karin N,Wildbaum G,Thelen M.Biased signaling pathways via CXCR3 control the development and function of CD4+ T cell subsets[J].J Leukoc Biol,2016,99(6):857-862.