微波辅助提取国槐籽中蛋白质的工艺研究
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摘要
以脱脂国槐籽粕为原料,采用微波辅助-溶剂浸提法提取国槐籽中的蛋白质,利用单因素实验考察了提取液浓度、料液比、提取温度、提取时间、微波功率等因素对蛋白质提取率的影响.通过正交试验和响应面试验法对比优化工艺条件.结果表明:当氢氧化钠浓度为3.5%、料液比1∶40(g∶mL)、提取温度为60℃、提取时间为35min、微波功率为95W时,国槐籽蛋白质的提取效果最佳,提取率为26.25%.
The protein in Sophora japonica seed was extracted by microwave-assisted extraction.The effects of concentration,solid-liquid ratio,extraction temperature,extraction time and microwave power on the extraction rate of protein were investigated with single factor test.The best extraction conditions were determined and analyzed compared by orthogonal test and response surface test.The optimum extraction process was as follows:the concentration of sodium hydroxide was 3.5%,solid-liquid ratio 1∶40(g∶mL),extracting temperatureat 60℃,extracting for 35 min and microwave power was 95 W.In this optimum extracion conditions,the extraction rate of protein was 26.25%.
The protein in Sophora japonica seed was extracted by microwave-assisted extraction.The effects of concentration,solid-liquid ratio,extraction temperature,extraction time and microwave power on the extraction rate of protein were investigated with single factor test.The best extraction conditions were determined and analyzed compared by orthogonal test and response surface test.The optimum extraction process was as follows:the concentration of sodium hydroxide was 3.5%,solid-liquid ratio 1∶40(g∶mL),extracting temperatureat 60℃,extracting for 35 min and microwave power was 95 W.In this optimum extracion conditions,the extraction rate of protein was 26.25%.
引文
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[2]李彩霞,郑雪,高海宁,等.国槐种子蛋白质提取工艺优化及其抗氧化活性[J].食品工业科技,2017,38(24):165-117.
[3]杨鑫,张秀省,穆红梅.国槐主要药用成分及提取方式研究进展[J].北方园艺,2011,(11):175-178.
[4]丛艳波,刘宁,张永忠.微波辅助提取槐角总异黄酮的研究[J].精细化工,2010,27(11):1083-1085.
[5]冉晓燕,胡德禹,薛伟.槐角中总黄酮的提取工艺研究[J].贵州教育学院学报,2009,20(6):22-24.
[6]杨云裳,蒋旺,张应鹏,等.酶法提取槐角中总黄酮的工艺优化[J].食品工业科技,2012,33(13):277-279.
[7]蔡志斌,刘俩燕,刘丽.微波消解技术在测定食品蛋白质含量中的应用[J].中国卫生检验杂志,2006,14(1):114-115.
[8]王艾平,周丽明.考马斯亮蓝法测定茶籽多糖中蛋白质含量条件的优化[J].河南农业科学,2014,43(3):150-153.
[9]严梅荣,鞠兴荣,王丹丹,等.碱提酸沉淀方法制取菜籽蛋白质的研究[J].中国粮油学报,2009,24(6):72-75.
[10]赵海霞,王晨,刘娅,等.打瓜籽蛋白质提取工艺的优化[J].食品工业,2015,36(8):95-99.
[11]梁丽敏,徐勇.微波辅助提取玉米醇溶蛋白方法的研究[J].食品科技,2014,39(3):128-131.
[12]李芳,孙乾,张爱琴,等.从辣椒籽粕中提取蛋白质的工艺研究[J].食品工业,2018,39(10):24-26.
[13]尹佳,王璐,孙健英,等.水酶法提取葡萄籽中蛋白质工艺的研究[J].中国酿造,2015,34(12):122-126.
[14]徐美,韩晓梅,范小振,等.超声波-微波协同提取碱蓬中总黄酮的工艺研究[J].黑龙江畜牧兽医,2018,(19):157-162.