摘要
目的检测Krupple样因子6 (Krupple-like factor 6,KLF6)及诱导型一氧化氮合酶(inducible nitric oxide synthase,i NOS)在铜绿假单胞菌(pseudomonas aeruginosa,PA)上清液感染的小鼠单核巨噬细胞白血病细胞(RAW264. 7)中的表达水平,探讨KLF6及i NOS在PA上清液感染RAW264. 7凋亡过程中的作用。方法 MTT法检测PA上清液对RAW264. 7增殖的影响;流式细胞术检测凋亡率; Hoechst 33342染色检测RAW264. 7细胞核形态变化; Western blot检测PA上清液对KLF6、i NOS表达的影响。结果 PA上清液抑制RAW264. 7的增殖,且其抑制作用呈浓度和时间依赖性,PA上清液能够明显增加RAW264. 7的凋亡比例,促进KLF6、i NOS的表达。结论 PA能够抑制RAW264. 7的增殖,其可能通过增加KLF6及i NOS的表达,从而诱导细胞凋亡。
Aim To detect the expression of Krupplelike factor 6( KLF6) and inducible nitric oxide synthase( i NOS) in mouse mononuclear macrophage leukemia cells infected with pseudomonas aeruginosa( PA) supernatant,and to investigate the role of KLF6 protein and i NOS protein in the apoptosis of RAW264. 7 infected with PA supernatant. Methods The effect of PA supernatant on the proliferation of RAW264. 7 was detected by MTT assay. The apoptotic rate was detected by flow cytometry. The morphological changes of RAW264. 7 were detected by Hoechst33342 staining. The effect of PA supernatant on KLF6and i NOS protein expression was detected by Western blot. Results PA supernatant inhibited the proliferation of RAW264. 7 in a concentration-and time-dependent manner. PA supernatant significantly increased the percentage of RAW264. 7 apoptosis and promoted the expression of KLF6 and i NOS. Conclusions PA can inhibit the proliferation of RAW264. 7,which may induce apoptosis through KLF6 and i NOS protein expression.
引文
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