评价血细胞比容、维生素C和半乳糖等干扰因素对3种便携式血糖仪的影响
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摘要
目的评价血细胞比容(HCT)、维生素C(Vc)和半乳糖等干扰因素对3种便携式血糖仪的影响,为临床选择合适的血糖仪提供一定参考。方法选取该院检验科肝素锂抗凝的静脉血标本20份用于血糖仪准确度验证,选取2名健康志愿者的空腹静脉全血标本各5份用于干扰物试验,参考罗氏C501全自动生化分析仪的检测结果,对Nova StatStrip Xpress血糖仪、拜安进血糖仪、罗氏卓越血糖仪进行准确度验证,评价HCT、Vc和半乳糖对以上3种便携式血糖仪检测结果的影响。结果 Nova StatStrip Xpress血糖仪和罗氏卓越血糖仪检测准确度均符合ISO15197∶2013标准要求,拜安进血糖仪检测准确度仅符合ISO15197∶2003标准要求。在不同水平HCT、Vc、半乳糖干扰下,Nova StatStrip Xpress血糖仪检测结果均符合ISO15197∶2013标准要求;拜安进血糖仪检测低浓度血糖时受到10mg/dL Vc干扰,其余检测结果均符合ISO15197∶2003标准要求;罗氏卓越血糖仪对HCT的抗干扰性能符合ISO15197∶2013标准要求,但受Vc和半乳糖干扰较大。结论 3种便携式血糖仪的准确度和抗干扰性能不同,临床应根据患者实际情况选择合适的血糖仪,确保血糖检测的准确性。
Objective To evaluate the influence of hematocrit(HCT),vitamin C(Vc)and galactose on three portable blood glucose meters to provide some reference for clinical selection of appropriate blood glucose meter.Methods 20 heparin anticoagulant venous blood samples in the clinical laboratory department of Nanfang Hospital were selected for verifying the accuracy of blood glucose meter.2 healthy volunteers were selected for collecting 5 whole blood samples in interferent test.Referring to the detection results of Roche automatic biochemical analyzer,the Nova StatStrip Xpress glucometer,Bayer Contour Plus glucometer and Roche Accu-chek Performa blood glucose meter were performed the accuracy verification.The influence of HCT,Vc and galactose on the detection results of above three portable blood glucose meters were evaluated.Results The detection accuracy of Nova StatStrip Xpress and the Roche Accu-Chek glucometers all conformed to the requirements of ISO15197∶2013 standards.The detection accuracy of Bayer Contour Plus glucometer only conformed to the requirements of ISO15197∶2003 standards.Under the interference of different levels of HCT,Vc and galactose,the detection results of Nova StatStrip Xpress glucometer conformed to the requirements of ISO15197:2013 standards;when detecting low concentration of blood glucose,the Bayer Contour Plus glucometer was interfered by 10 mg/dL Vc,and other detection results conformed to the requirements of ISO15197∶2003 standards;the anti-interference performance of Roche Accu-chek Performa glucometer conformed to the requirements of ISO15197∶2013 standards,but the Vc and galactose interference was relatively great.Conclusion The accuracy and anti-interference performance have difference among the three portable blood glucose meters.Clinic should select suitable blood glucose meter according to the actual situation of the patients to ensure the accuracy of blood glucose detection.
Objective To evaluate the influence of hematocrit(HCT),vitamin C(Vc)and galactose on three portable blood glucose meters to provide some reference for clinical selection of appropriate blood glucose meter.Methods 20 heparin anticoagulant venous blood samples in the clinical laboratory department of Nanfang Hospital were selected for verifying the accuracy of blood glucose meter.2 healthy volunteers were selected for collecting 5 whole blood samples in interferent test.Referring to the detection results of Roche automatic biochemical analyzer,the Nova StatStrip Xpress glucometer,Bayer Contour Plus glucometer and Roche Accu-chek Performa blood glucose meter were performed the accuracy verification.The influence of HCT,Vc and galactose on the detection results of above three portable blood glucose meters were evaluated.Results The detection accuracy of Nova StatStrip Xpress and the Roche Accu-Chek glucometers all conformed to the requirements of ISO15197∶2013 standards.The detection accuracy of Bayer Contour Plus glucometer only conformed to the requirements of ISO15197∶2003 standards.Under the interference of different levels of HCT,Vc and galactose,the detection results of Nova StatStrip Xpress glucometer conformed to the requirements of ISO15197:2013 standards;when detecting low concentration of blood glucose,the Bayer Contour Plus glucometer was interfered by 10 mg/dL Vc,and other detection results conformed to the requirements of ISO15197∶2003 standards;the anti-interference performance of Roche Accu-chek Performa glucometer conformed to the requirements of ISO15197∶2013 standards,but the Vc and galactose interference was relatively great.Conclusion The accuracy and anti-interference performance have difference among the three portable blood glucose meters.Clinic should select suitable blood glucose meter according to the actual situation of the patients to ensure the accuracy of blood glucose detection.
引文
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[10]石文,陈富,邱峰,等.用EP-7A文件评价维生素C对两种微量血糖仪的干扰[J].现代检验医学杂志,2010,25(6):156-157.
[11]KAHN S A,LENTZ C W.Fictitious hyperglycemia:point-of-care glucose measurement is inaccurate during high-dose vitamin C infusion for burn shock resuscitation[J].J Burn Care Res,2015,36(2):e67-e71.
[12]纪昕,王鑫,岳晓乐,等.3种便携式血糖检测仪的分析性能评价[J].检验医学与临床,2016,13(7):917-919.
[13]乐建培,王炳华,林勇.血细胞比容和维生素C对便携式血糖仪检测结果的影响[J].实验与检验医学,2016,34(3):331-334.
[14]解宏杰,邱玲,国秀芝,等.四种型号便携式血糖仪的准确度验证[J].现代检验医学杂志,2013,28(2):113-117.
[15]王淼,周染云,杨兴龙,等.医院POCT血糖仪应用现状分析[J].中华检验医学杂志,2016,39(8):643-645.
[2]廖远泉,廖安琪.POCT-便携式血糖仪及其准确性影响因素研究概述[J/CD].临床检验杂志(电子版),2015,4(2):876-881.
[3]梅松,张家,顾光煜,等.不同分析原理POCT血糖仪的测定结果分析[J].国际检验医学杂志,2013,34(21):2906-2907.
[4]王煜非.解读ISO15197:2013标准[J].中国糖尿病杂志,2014,22(12):1149-1152.
[5]陈雪梅.红细胞压积对血糖仪与全自动生化仪检测末梢血和静脉血血糖一致性的影响[J].中国医学装备,2016,13(10):92-94.
[6]李小斌,杨阳,张士朋,等.POCT血糖仪、生化仪葡萄糖氧化酶法和己糖激酶法检测新生儿血糖的研究[J].实用预防医学,2012,19(9):1395-1398.
[7]陈芳华,饶万楷.4种POCT血糖仪的性能比较[J].重庆医科大学学报,2011,36(1):68-72.
[8]艾承锦,廖明星,潘瑞琪,等.便携式血糖仪系统准确性评价及血细胞比容对其检测结果的影响[J].检验医学与临床,2015,12(14):2003-2005.
[9]KARON B S,GRIESMAN L,SCOTT R,et al.Evaluation of the impact of hematocrit and other interference on the accuracy of hospital-based glucose meters[J].Diabetes Technol Ther,2008,10(2):111-120.
[10]石文,陈富,邱峰,等.用EP-7A文件评价维生素C对两种微量血糖仪的干扰[J].现代检验医学杂志,2010,25(6):156-157.
[11]KAHN S A,LENTZ C W.Fictitious hyperglycemia:point-of-care glucose measurement is inaccurate during high-dose vitamin C infusion for burn shock resuscitation[J].J Burn Care Res,2015,36(2):e67-e71.
[12]纪昕,王鑫,岳晓乐,等.3种便携式血糖检测仪的分析性能评价[J].检验医学与临床,2016,13(7):917-919.
[13]乐建培,王炳华,林勇.血细胞比容和维生素C对便携式血糖仪检测结果的影响[J].实验与检验医学,2016,34(3):331-334.
[14]解宏杰,邱玲,国秀芝,等.四种型号便携式血糖仪的准确度验证[J].现代检验医学杂志,2013,28(2):113-117.
[15]王淼,周染云,杨兴龙,等.医院POCT血糖仪应用现状分析[J].中华检验医学杂志,2016,39(8):643-645.