花生辐照变异新品系的选育
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摘要
为丰富花生遗传基础,克服栽培遗传多样性差、高产优质专用新品种培育难以获得突破性进展的困难,本试验以花生品种鲁花11号干种子为诱变材料,采用~(60)Co-γ射线辐照处理,研究突变体后代性状表现。结果表明,突变体Y-21的M_2明显变异和分离,部分单株荚果明显变大,内种皮突变为金黄色。后代跟踪观察发现大果和金黄色内种皮能够遗传。对M7产量和品质的鉴定结果表明,从Y-21后代中选出的2个品系(大果和金黄色内种皮)Y-21-2-4和Y-21-3-2,其荚果形状、大小,籽仁形状,种皮和内种皮颜色等均符合传统出口型大花生的要求,且产量较高,其中Y-21-2-4比鲁花11号增产13.79%,比山东省区对照品种花育25号增产6.19%~7.70%,粗脂肪含量比鲁花11号高1.8个百分点,比花育25号高5.1个百分点。本研究获得的突变品系为花生育种及遗传研究提供了材料。
The seeds of peanut variety Luhua 11 were used as materials for irradiation treatment by ~(60)Co-γ ray,and characteristics of the mutation progeny were studied,aiming at broading peanut genetic resources and breeding new peanut varieties. Obvious variations and segregations were observed in M_2 generation from mutant Y-21,and some single plants with bigger pod and golden yellow endopleura were found to keep stable inheritance in M_3 generation. The yield and quality characteristics were evaluated and detected in M_7 generation. The characteristics of pod shape,pod size,kernel shape,color of testa and endopleura from two lines Y-21-2-4 and Y-21-3-2 could meet the standard of traditional export peanut. Y-21-2-4 and Y-21-3-2 lines also showed high yield,the yield of Y-21-2-4 was 13. 79% higher than that of mutagenic parent Luhua 11 6. 19% to 7. 70% higher than Huayu 25 the control variety in Shandong regional tests. The oil content of Y-21-2-4 was 1. 80% and 5. 10% higher than that of Luhua 11 and Huayu 25 respectively. These newly obtained mutant lines will be used as materials in genetic study and breeding in peanut.
The seeds of peanut variety Luhua 11 were used as materials for irradiation treatment by ~(60)Co-γ ray,and characteristics of the mutation progeny were studied,aiming at broading peanut genetic resources and breeding new peanut varieties. Obvious variations and segregations were observed in M_2 generation from mutant Y-21,and some single plants with bigger pod and golden yellow endopleura were found to keep stable inheritance in M_3 generation. The yield and quality characteristics were evaluated and detected in M_7 generation. The characteristics of pod shape,pod size,kernel shape,color of testa and endopleura from two lines Y-21-2-4 and Y-21-3-2 could meet the standard of traditional export peanut. Y-21-2-4 and Y-21-3-2 lines also showed high yield,the yield of Y-21-2-4 was 13. 79% higher than that of mutagenic parent Luhua 11 6. 19% to 7. 70% higher than Huayu 25 the control variety in Shandong regional tests. The oil content of Y-21-2-4 was 1. 80% and 5. 10% higher than that of Luhua 11 and Huayu 25 respectively. These newly obtained mutant lines will be used as materials in genetic study and breeding in peanut.
引文
[1]王晶珊,赵明霞,乔利仙,隋炯明,孔福全,王潇,刘录祥.快中子辐照对花生种子胚小叶植株再生的影响[J].中国油料作物学报,2013,35(2):148-152
[2]吴兰荣,李正超,邱庆树,苗华荣.我国花生诱变育种技术应用研究概况[J].核农学报,2002,16(5):334-336
[3]敖妍,潘青华.60Co-γ射线辐照扶芳藤枝条的表型变异研究[J].核农学报,2008,22(3):271-275
[4]王鹏,刘录祥,纪瑞瑞,隋炯明,乔利仙,郭宝太,孔祥远,王晶珊.60Co-γ射线辐照对甘薯农艺性状变异的影响[J].植物生理学报,2015,51(7):1167-1172
[5]李树发,张颢,邱显钦,葛红,蹇洪英,王其刚,晏慧君,唐开学.切花月季60Co-γ辐照诱变育种初报[J].核农学报,2011,25(4):713-718
[6]邱庆树,李正超.花生辐照与杂交相结合的育种效果研究[J].中国农业科学,1999,32(2):41-44
[7]李正超,邱庆树,吴兰荣,胡文广,苗华荣.辐射与杂交相结合选育大花生新品种花育16号的研究[J].核农学报,2001,15(6):368-370
[8]吴如健,黄镜浩,温寿星,蔡子坚,罗土炎,陈良锋,王周文.航天搭载后福橘(Citrusreticulate Blanco)茎尖无性系有丝分裂行为和细胞超微结构的变化[J].核农学报,2011,25(6):259-265
[9]于新玲,刘录祥,乔利仙,隋炯明,郭宝太,徐丽娟,王晶珊.高能混合离子场辐照对花生胚小叶组织培养及植株再生的影响[J].核农学报,2012,26(3):433-438
[10]Wang J S,Sui J M,Xie Y D,Guo H J,Qiao L X,Zhao L L,Yu S L,Liu L X.Generation of peanut mutants by fast neutron irradiation combined with in vitro culture[J].Journal of Radiation Research,2015,56(3):437-445
[11]Zhao M X,Sun H Y,Ji R R,Hu X H,Sui J M,Qiao L X,Chen J,Wang J S.In vitro mutagenesis and directed screening for salttolerant mutants in peanut[J].Euphytica,2013,193:89-99
[12]Sui J M,Wang Y,Wang P,Qiao L X,Sun S M,Hu X H,Chen J,Wang J S.Generation of peanut drought tolerant plants by pingyangmycin-mediated in vitro mutagenesis and hydroxyprolineresistance screening[J].PLo S One,2015,10(3):e0119240
[13]万勇善.高产优质抗病大花生新品种-丰花1号[J].河北农业科学,2001,5(3):70-71
[14]吴兰荣,陈静,石运庆,苗华荣,齐卫,陈效东,胡文广.60Co-γ射线诱变与杂交相结合选育花生新品种-花育22号[J].核农学报,2006,20(4):309-311
[15]范家霖,张建伟,杨保安,张福彦,胡秀菊,程仲杰.河南省小麦诱变育种进展与分析[J].麦类作物学报,2013,33(1):195-199
[16]王鹏,刘录祥,纪瑞瑞,隋炯明,乔利仙,郭宝太,孔祥远,王晶珊.60Co-γ射线辐照对甘薯农艺性状变异的影响[J].植物生理学报,2015,51(7):1167-1172
[17]谢向誉,陆柳英,曾文丹,严华兵.木薯诱变育种研究进展[J].农学学报,2014,4(2):34-38
[18]Wang J S,Qiao L X,Zhao L S,Wang P,Guo B T,Liu L X,Sui J M.Performance of peanut mutants and their offspring generated from mixed high-energy particle field radiation and tissue culture[J].Genetics and Molecular Research,2015,14(3):10837-10848
[19]张勇,杨兴勇,董全中,薛红,张明明,李微微,宋继玲,许德春,孟丽芬,付立新,赵晓南,胡少新,刘录祥.利用空间诱变技术选育大豆新品种克山1号[J].核农学报,2013,27(9):1241-1246
[20]肖鑫丽,刘京宏,尹德松,宋静武,彭磊.辐射在园艺植物诱变育种中的应用研究进展[J].贵州农业科学,2015,43(1):20-23
[21]孔广红,柳觐,倪书邦,范秋红,宫丽丹,陶亮,陶丽,陈丽兰,贺熙勇.澳洲坚果接穗60Co-γ射线辐射诱变育种适宜剂量的研究[J].西南农业学报,2016,29(1):39-43
[22]杜文平,宋军,徐利远,陈谦,张莲,余桂容.60Co-γ射线对小麦幼胚离体后的诱变效应[J].西南农业学报,2015,28(6):2355-2359
[23]张从字,王敏,李文阳,崔嘉欣,王停停.60Co-γ射线对皖麦50 M2代农艺及品质性状的诱变效应[J].种子,2014,33(12):80-83
[24]何平,李林光,王海波,李慧峰,常源升,程来亮.辐照处理对苹果嫩枝变异效应研究[J].核农学报,2016,30(7):1249-1254
[25]贾新旺.制约花生产业发展的因素及对策[N].河北科技报,2009-11-26(12)
[26]陈光尧,王国槐,罗峰,聂明建,王济人.60Co-γ辐照对甘蓝型油菜农艺性状和品质的影响[J].华北农学报,2007,22(5):56-59
[27]苗荣华,李培习,胡晓辉,石运庆,陈静.60Co-γ射线辐照鲁花11号对后代品质的影响[J].山东农业科学,2009(10):39-42
[28]陈静,胡晓辉,苗华荣,石运庆,崔凤高,吴兰荣,孙令强,禹山林.60Co-γ射线辐照花生种子后代的SSR分析[J].华北农学报,2010,25(3):68-72
[2]吴兰荣,李正超,邱庆树,苗华荣.我国花生诱变育种技术应用研究概况[J].核农学报,2002,16(5):334-336
[3]敖妍,潘青华.60Co-γ射线辐照扶芳藤枝条的表型变异研究[J].核农学报,2008,22(3):271-275
[4]王鹏,刘录祥,纪瑞瑞,隋炯明,乔利仙,郭宝太,孔祥远,王晶珊.60Co-γ射线辐照对甘薯农艺性状变异的影响[J].植物生理学报,2015,51(7):1167-1172
[5]李树发,张颢,邱显钦,葛红,蹇洪英,王其刚,晏慧君,唐开学.切花月季60Co-γ辐照诱变育种初报[J].核农学报,2011,25(4):713-718
[6]邱庆树,李正超.花生辐照与杂交相结合的育种效果研究[J].中国农业科学,1999,32(2):41-44
[7]李正超,邱庆树,吴兰荣,胡文广,苗华荣.辐射与杂交相结合选育大花生新品种花育16号的研究[J].核农学报,2001,15(6):368-370
[8]吴如健,黄镜浩,温寿星,蔡子坚,罗土炎,陈良锋,王周文.航天搭载后福橘(Citrusreticulate Blanco)茎尖无性系有丝分裂行为和细胞超微结构的变化[J].核农学报,2011,25(6):259-265
[9]于新玲,刘录祥,乔利仙,隋炯明,郭宝太,徐丽娟,王晶珊.高能混合离子场辐照对花生胚小叶组织培养及植株再生的影响[J].核农学报,2012,26(3):433-438
[10]Wang J S,Sui J M,Xie Y D,Guo H J,Qiao L X,Zhao L L,Yu S L,Liu L X.Generation of peanut mutants by fast neutron irradiation combined with in vitro culture[J].Journal of Radiation Research,2015,56(3):437-445
[11]Zhao M X,Sun H Y,Ji R R,Hu X H,Sui J M,Qiao L X,Chen J,Wang J S.In vitro mutagenesis and directed screening for salttolerant mutants in peanut[J].Euphytica,2013,193:89-99
[12]Sui J M,Wang Y,Wang P,Qiao L X,Sun S M,Hu X H,Chen J,Wang J S.Generation of peanut drought tolerant plants by pingyangmycin-mediated in vitro mutagenesis and hydroxyprolineresistance screening[J].PLo S One,2015,10(3):e0119240
[13]万勇善.高产优质抗病大花生新品种-丰花1号[J].河北农业科学,2001,5(3):70-71
[14]吴兰荣,陈静,石运庆,苗华荣,齐卫,陈效东,胡文广.60Co-γ射线诱变与杂交相结合选育花生新品种-花育22号[J].核农学报,2006,20(4):309-311
[15]范家霖,张建伟,杨保安,张福彦,胡秀菊,程仲杰.河南省小麦诱变育种进展与分析[J].麦类作物学报,2013,33(1):195-199
[16]王鹏,刘录祥,纪瑞瑞,隋炯明,乔利仙,郭宝太,孔祥远,王晶珊.60Co-γ射线辐照对甘薯农艺性状变异的影响[J].植物生理学报,2015,51(7):1167-1172
[17]谢向誉,陆柳英,曾文丹,严华兵.木薯诱变育种研究进展[J].农学学报,2014,4(2):34-38
[18]Wang J S,Qiao L X,Zhao L S,Wang P,Guo B T,Liu L X,Sui J M.Performance of peanut mutants and their offspring generated from mixed high-energy particle field radiation and tissue culture[J].Genetics and Molecular Research,2015,14(3):10837-10848
[19]张勇,杨兴勇,董全中,薛红,张明明,李微微,宋继玲,许德春,孟丽芬,付立新,赵晓南,胡少新,刘录祥.利用空间诱变技术选育大豆新品种克山1号[J].核农学报,2013,27(9):1241-1246
[20]肖鑫丽,刘京宏,尹德松,宋静武,彭磊.辐射在园艺植物诱变育种中的应用研究进展[J].贵州农业科学,2015,43(1):20-23
[21]孔广红,柳觐,倪书邦,范秋红,宫丽丹,陶亮,陶丽,陈丽兰,贺熙勇.澳洲坚果接穗60Co-γ射线辐射诱变育种适宜剂量的研究[J].西南农业学报,2016,29(1):39-43
[22]杜文平,宋军,徐利远,陈谦,张莲,余桂容.60Co-γ射线对小麦幼胚离体后的诱变效应[J].西南农业学报,2015,28(6):2355-2359
[23]张从字,王敏,李文阳,崔嘉欣,王停停.60Co-γ射线对皖麦50 M2代农艺及品质性状的诱变效应[J].种子,2014,33(12):80-83
[24]何平,李林光,王海波,李慧峰,常源升,程来亮.辐照处理对苹果嫩枝变异效应研究[J].核农学报,2016,30(7):1249-1254
[25]贾新旺.制约花生产业发展的因素及对策[N].河北科技报,2009-11-26(12)
[26]陈光尧,王国槐,罗峰,聂明建,王济人.60Co-γ辐照对甘蓝型油菜农艺性状和品质的影响[J].华北农学报,2007,22(5):56-59
[27]苗荣华,李培习,胡晓辉,石运庆,陈静.60Co-γ射线辐照鲁花11号对后代品质的影响[J].山东农业科学,2009(10):39-42
[28]陈静,胡晓辉,苗华荣,石运庆,崔凤高,吴兰荣,孙令强,禹山林.60Co-γ射线辐照花生种子后代的SSR分析[J].华北农学报,2010,25(3):68-72