航苜1号紫花苜蓿多叶性状遗传特性及分子标记检测
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摘要
以航天诱变选育的多叶型紫花苜蓿新品种"航苜1号"为对象,简要阐述了其选育过程、连续四代多叶性状的遗传规律及多叶率、草产量、营养成分、氨基酸、微量元素等指标与对照相比较的优越性。同时,为检测航天搭载后第一代(SP1)植株是否发生基因变异及在SP2~SP4代变异能否稳定遗传,连续四代分别采样,采用SRAP分子标记法进行检测,结果表明:SP1代与未搭载原品种(CK)相比基因组DNA扩增出不同的差异带,分别为缺失1条带,大小约550bp,增加1条带,大小约100bp,在DNA水平上产生了变异,并且在SP2、SP3、SP4代稳定遗传。
A new variety of‘Hangmu No.1'was breed by space mutation,its breeding process and hereditary of four generation were briefly introduced.The superiority of multi-leaf,grass yield,nutritional content and amino acids content were studied compared with control variety.SRAP was employed to test whether the gene of SP1 generation was varied and could inherit stably in SP2~SP4.The results showed that there were differences in amplified genomic DNA bands between SP1 generation and CK,one band about 550 bp was lost and one new band about 100 bp appeared,this mutation can continue heredity stably in the SP2,SP3,SP4 generation.
A new variety of‘Hangmu No.1'was breed by space mutation,its breeding process and hereditary of four generation were briefly introduced.The superiority of multi-leaf,grass yield,nutritional content and amino acids content were studied compared with control variety.SRAP was employed to test whether the gene of SP1 generation was varied and could inherit stably in SP2~SP4.The results showed that there were differences in amplified genomic DNA bands between SP1 generation and CK,one band about 550 bp was lost and one new band about 100 bp appeared,this mutation can continue heredity stably in the SP2,SP3,SP4 generation.
引文
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[2]袁庆华,桂枝,张文.苜蓿基因组DNA提取和RAPD反应条件优化[J].草地学报,2001,9(2):99-105.Yuan Qinghua,Gui Zhi,Zhang Wen.Alfalfa genomic DNA extraction and optimization of RAPD reaction conditions[J].Acta Agrestia Sinica,2001,9(2):99-105.
[3]范润钧,邓波,陈本建,等.航天搭载紫花苜蓿连续后代变异株系选育[J].山西农业科学,2010,38(5):7-9.Fan Runjun,Deng Bo,Chen Benjian,et al.Selection of successive progean mutants by space flight in purple alfalfa[J].Journal of Shanxi Agricultural Sciences,2010,38(5):7-9.
[4]范润钧.空间搭载紫花苜蓿种子第一代枝株表型变异及基因多态性分析[D].兰州:甘肃农业大学,2010.Fan Runjun.Phenotypic variation and genomic polymorphism in the first generation from alfalfa seeds mutagenized on spaceship[D].Lanzhou:Gansu Agricultural University,2010.
[5]王密,魏建民,郭慧琴,等.紫花苜蓿空间诱变突变体筛选及其RAPD多态性分析(简报)[J].草地学报,2009,17(6):841-844.Wang Mi,Wei Jianmin,Guo Huiqing,et al.Selection of space flight mutation of alfalfa and its polymorphic analysis by RAPD[J].Acta Agrestia Sinica,2009,17(6):841-844.
[6]王密.紫花苜蓿种子空间诱变变异效应的研究[D].呼和浩特:内蒙古农业大学,2010.Wang Mi.Mutation effect of space flight factors on alfalfa seeds[D].Hohhot:Inner Mongolia Agricultural University,2010.
[7]杜连莹.实践八号搭载8个苜蓿品种生物学效应研究[D].哈尔滨:哈尔滨师范大学,2010.Du Lianying.The biology effect study of Medicago sativa L.carried by“Shijian No.8”recoverable satellite[D].Harbin:Harbin Normal University,2010.