摘要
为了获得高效降胆固醇乳酸菌菌株并研究日常生活中的金属离子对乳酸菌降胆固醇的影响作用,从富含胆固醇的材料中,分离获得14株疑似乳酸菌,通过已获得的4株乳酸菌比较,从中筛选到2株胆固醇降解率最高的乳酸菌,分别为植物乳杆菌(Lactobacillus plantarum,Lpl)和鼠李糖乳杆菌(Lactobacillus rhamnosus,Lrh)。结果显示除了在Ca~(2+)浓度为0.02 mol/L时两株菌降解率出现升高之外,Lrh和Lpl的胆固醇降解率均随着金属离子浓度的增加而降低。然而,Lrh和Lpl菌株在添加Li+、K+、Ca~(2+)和Al~(3+)的MRS-CHOL培养基中,当金属离子浓度为0.02 mol/L时,其胆固醇降解效力最高,而后随着浓度升高,降解效力逐渐降低;而添加Mn~(2+)、Cu~(2+)和Fe~(3+)时,胆固醇降解效力一直是随着离子浓度的增加而降低。
In order to obtain high efficient cholesterol degradation lactic acid bacteria(LAB)and research the effect of common metal ions on their cholesterol decomposition function,this study attempted to isolate LAB from cholesterol rich material. The cholesterol-degradation percentages of 14 suspected LAB strains isolated from food samples and 4 strains LAB in our lab were analyzed. Two higher efficient cholesterol degradation strains,1 strain of Lactobacillus plantarum(Lpl)and 1 strain of Lactobacillus rhamnosus(Lrh)were chosen out to research the effects of metal ions on their cholesterol-reduction. Results showed that the cholesterol degradation efficiency of two strains were enhanced only under additional 0.02 mol/L concentration of Ca~(~(2+)),but decreased with the increase of other metal ions concentration. However,the cholesterol efficacy of both Lrh and Lpl were highest under extra 0.02 mol/L of Li+,K+,Ca~(2+) and Al~(3+) of MRS-CHOL medium and gradually reduced with the concentration increase. Meanwhile,the cholesterol efficacy of two strains always decreased with the increase of concentration of additional Mn~(2+),Cu~(2+) and Fe~(3+).
引文
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