金属离子对乳酸菌降解胆固醇的影响
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摘要
为了获得高效降胆固醇乳酸菌菌株并研究日常生活中的金属离子对乳酸菌降胆固醇的影响作用,从富含胆固醇的材料中,分离获得14株疑似乳酸菌,通过已获得的4株乳酸菌比较,从中筛选到2株胆固醇降解率最高的乳酸菌,分别为植物乳杆菌(Lactobacillus plantarum,Lpl)和鼠李糖乳杆菌(Lactobacillus rhamnosus,Lrh)。结果显示除了在Ca~(2+)浓度为0.02 mol/L时两株菌降解率出现升高之外,Lrh和Lpl的胆固醇降解率均随着金属离子浓度的增加而降低。然而,Lrh和Lpl菌株在添加Li+、K+、Ca~(2+)和Al~(3+)的MRS-CHOL培养基中,当金属离子浓度为0.02 mol/L时,其胆固醇降解效力最高,而后随着浓度升高,降解效力逐渐降低;而添加Mn~(2+)、Cu~(2+)和Fe~(3+)时,胆固醇降解效力一直是随着离子浓度的增加而降低。
In order to obtain high efficient cholesterol degradation lactic acid bacteria(LAB)and research the effect of common metal ions on their cholesterol decomposition function,this study attempted to isolate LAB from cholesterol rich material. The cholesterol-degradation percentages of 14 suspected LAB strains isolated from food samples and 4 strains LAB in our lab were analyzed. Two higher efficient cholesterol degradation strains,1 strain of Lactobacillus plantarum(Lpl)and 1 strain of Lactobacillus rhamnosus(Lrh)were chosen out to research the effects of metal ions on their cholesterol-reduction. Results showed that the cholesterol degradation efficiency of two strains were enhanced only under additional 0.02 mol/L concentration of Ca~(~(2+)),but decreased with the increase of other metal ions concentration. However,the cholesterol efficacy of both Lrh and Lpl were highest under extra 0.02 mol/L of Li+,K+,Ca~(2+) and Al~(3+) of MRS-CHOL medium and gradually reduced with the concentration increase. Meanwhile,the cholesterol efficacy of two strains always decreased with the increase of concentration of additional Mn~(2+),Cu~(2+) and Fe~(3+).
In order to obtain high efficient cholesterol degradation lactic acid bacteria(LAB)and research the effect of common metal ions on their cholesterol decomposition function,this study attempted to isolate LAB from cholesterol rich material. The cholesterol-degradation percentages of 14 suspected LAB strains isolated from food samples and 4 strains LAB in our lab were analyzed. Two higher efficient cholesterol degradation strains,1 strain of Lactobacillus plantarum(Lpl)and 1 strain of Lactobacillus rhamnosus(Lrh)were chosen out to research the effects of metal ions on their cholesterol-reduction. Results showed that the cholesterol degradation efficiency of two strains were enhanced only under additional 0.02 mol/L concentration of Ca~(~(2+)),but decreased with the increase of other metal ions concentration. However,the cholesterol efficacy of both Lrh and Lpl were highest under extra 0.02 mol/L of Li+,K+,Ca~(2+) and Al~(3+) of MRS-CHOL medium and gradually reduced with the concentration increase. Meanwhile,the cholesterol efficacy of two strains always decreased with the increase of concentration of additional Mn~(2+),Cu~(2+) and Fe~(3+).
引文
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[22]Feng M,Chen X,Li C,et al.Isolation and Identification of an Exopolysaccharide-Producing Lactic Acid Bacterium Strain from Chinese Paocai and Biosorption of Pb(II)by Its Exopolysaccharide[J].Journal of Food Science,2012,77(6):111-117
[23]朱晓.乳酸菌表层蛋白的性质、结构与功能[D].无锡:江南大学,2012
[24]Bhakta J N,Ohnishi K,Munekage Y,et al.Characterization of lactic acid bacteria-based probiotics as potential heavy metal sorbents[J].Journal of Applied Microbiology,2012,112(6):1193-1206
[25]Tong Y,Wang G,Zhang Q,et al.Systematic understanding of the potential manganese-adsorption components of a screened Lactobacillus plantarum CCFM436[J].Rsc Advances,2016,6:102804-102813
[2]Manson J A E,Tosteson H,Ridker P M,et al.Review article:The primary prevention of myocardial infarction[J].New England Journal of Medicine,1992,326(21):1406-1416
[3]Kunnen S,Van E M.Lecithin:cholesterol acyltransferase:old friend or foe in atherosclerosis[J].Journal of Lipid Research,2012,53(9):1783-1799
[4]Anderson J W,Gilliland S E.Effect of Fermented Milk(Yogurt)Containing Lactobacillus Acidophilus L1 on Serum Cholesterol in Hypercholesterolemic Humans[J].Journal of the American College of Nutrition,1999,18(1):43-50
[5]Feord J.Lactic acid bacteria in a changing legislative environment[J].Antonie Van Leeuwenhoek,2002,82(1-4):353-360
[6]Stile s M E,Holzapfel W H.Lactic acid bacteria of foods and their current taxonomy[J].International Journal of Food Microbiology,1997,36(1):1-29
[7]Wang C Y,Wu S,Fang J Y,et al.Cardiovascular and intestinal protection of cereal pastes fermented with lactic acid bacteria in hyperlipidemic hamsters[J].Food Research International,2012,48(2):428-434
[8]Jeun J,Kim S,Cho S Y,et al.Hypocholesterolemic effects of Lactobacillus plantarum KCTC3928 by increased bile acid excretion in C57BL/6 mice[J].Nutrition,2010,26(3):321-330
[9]Gilliland S E,Walker D K.Factors to Consider When Selecting a Culture of Lactobacillus acidophilus as a Dietary Adjunct to Produce a Hypocholesterolemic Effect in Humans[J].Journal of Dairy Science,1990,73(4):905-911
[10]Duggan C,Gannon J,Walker W A.Protective nutrients and functional foods for the gastrointestinal tract[J].American Journal of Clinical Nutrition,2002,75(5):789-808
[11]马欢欢,白凤翎,励建荣.乳酸菌吸附作用清除食品中有毒重金属研究进展[J].食品科学,2017,38(11):301-307
[12]李雪萍,李建宏,李敏权,等.浆水中降胆固醇乳酸菌的筛选及其功能特性[J].微生物学报,2015,55(8):1001-1009
[13]丁苗,刘洋,葛平珍,等.发酵酸肉中降胆固醇乳酸菌的筛选、鉴定及降胆固醇作用[J].食品科学,2014,35(19):203-207
[14]靳志敏,通力嘎,段艳,等.1株高效降胆固醇乳酸菌的功能与发酵特性[J].食品科学,2014,35(11):120-123
[15]Soda M E,Ahmed N,Omran N,et al.Isolation identification and selection of lactic acid bacteria cultures for cheese making[J].Emirates Journal of Food&Agriculture,2003,15(1):51-71
[16]Badis A,Guetarni D,Boudjema B M,et al.Identification and technological properties of lactic acid bacteria isolated from raw goat milk of four Algerian races[J].Food Microbiology,2004,21(5):579-588
[17]Feng H W,Pi C M,Wang R B,et al.Use of ferric ammonium sulfate in serum cholesterol determination[J].Clinical Chemistry,1973,19(1):121-122
[18]Angmo K,Kumari A,Savitri,et al.Probiotic characterization of lactic acid bacteria isolated from fermented foods and beverage of Ladakh[J].LWT-Food Science and Technology,2016,66:428-435
[19]Brown M S,Goldstein J L.How LDL receptors influence cholesterol and atherosclerosis[J].Scientific American,1984,251(5):58-66
[20]Khedkar C D,Mantri J M,Garge R D,et al.Hypocholesterolemic effect of fermented milks:a review[J].Cultured Dairy Products Journal,1993,28(3):14-18
[21]Miremadi F,Ayyash M,Sherkat F,et al.Cholesterol reduction mechanisms and fatty acid composition of cellular membranes of probiotic Lactobacilli and Bifidobacteria[J].Journal of Functional Foods,2014,9(7):295-305
[22]Feng M,Chen X,Li C,et al.Isolation and Identification of an Exopolysaccharide-Producing Lactic Acid Bacterium Strain from Chinese Paocai and Biosorption of Pb(II)by Its Exopolysaccharide[J].Journal of Food Science,2012,77(6):111-117
[23]朱晓.乳酸菌表层蛋白的性质、结构与功能[D].无锡:江南大学,2012
[24]Bhakta J N,Ohnishi K,Munekage Y,et al.Characterization of lactic acid bacteria-based probiotics as potential heavy metal sorbents[J].Journal of Applied Microbiology,2012,112(6):1193-1206
[25]Tong Y,Wang G,Zhang Q,et al.Systematic understanding of the potential manganese-adsorption components of a screened Lactobacillus plantarum CCFM436[J].Rsc Advances,2016,6:102804-102813