去折叠态β-乳球蛋白与表没食子儿茶素没食子酸酯的相互作用
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摘要
通过微波处理β-乳球蛋白(β-lactoglobulin,β-LG)得到去折叠态β-乳球蛋白(unfolded-β-lactoglobulin,U-β-LG),采用荧光光谱、紫外-可见光谱、圆二色光谱的方法研究表没食子儿茶素没食子酸酯(epigallocatechingallate,EGCG)与U-β-LG的相互作用机制。结果表明,EGCG能与β-LG和U-β-LG相互作用形成复合物。它们相互之间均主要为疏水作用力。在298 K时,EGCG与β-LG和U-β-LG的结合距离分别为3.188 nm和2.875 nm。EGCG的结合使β-LG和U-β-LG的二、三级结构发生变化,表面疏水性降低。与天然β-LG相比,U-β-LG与EGCG具有更大的结合强度,结合后的U-β-LG发生更大的结构变化。
The interaction mechanism of unfolded bovine β-lactoglobulin(U-β-LG) with epigallocatechin gallate(EGCG)was investigated by using fluorescence, ultraviolet and circular dichroism(CD) spectroscopy. U-β-LG was obtained afterβ-lactoglobulin(β-LG) was treated by microwave. It was shown that EGCG could interact with β-LG or U-β-LG to formβ-LG-EGCG complex or U-β-LG-EGCG complex. The main binding force between EGCG and β-LG or U-β-LG was hydrophobic interaction. The binding distance between donor and acceptor at 298 K were 3.188 and 2.875 nm for β-LGEGCG complex and U-β-LG-EGCG complex, respectively based on the F?rster's theory of non-radiative energy transfer.The two-and three-dimensional structures of β-LG and U-β-LG were changed and their surface hydrophobicity was decreased slightly after being combined with EGCG. The binding affinity between U-β-LG and EGCG was stronger than that between β-LG and EGCG, which contributed to greater conformational change of U-β-LG.
The interaction mechanism of unfolded bovine β-lactoglobulin(U-β-LG) with epigallocatechin gallate(EGCG)was investigated by using fluorescence, ultraviolet and circular dichroism(CD) spectroscopy. U-β-LG was obtained afterβ-lactoglobulin(β-LG) was treated by microwave. It was shown that EGCG could interact with β-LG or U-β-LG to formβ-LG-EGCG complex or U-β-LG-EGCG complex. The main binding force between EGCG and β-LG or U-β-LG was hydrophobic interaction. The binding distance between donor and acceptor at 298 K were 3.188 and 2.875 nm for β-LGEGCG complex and U-β-LG-EGCG complex, respectively based on the F?rster's theory of non-radiative energy transfer.The two-and three-dimensional structures of β-LG and U-β-LG were changed and their surface hydrophobicity was decreased slightly after being combined with EGCG. The binding affinity between U-β-LG and EGCG was stronger than that between β-LG and EGCG, which contributed to greater conformational change of U-β-LG.
引文
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[2]HOSSEINI S M H,EMAM-DJOMEH Z,SABATINO P,et al.Nanocomplexes arising from protein-polysaccharide electrostatic interaction as a promising carrier for nutraceutical compounds[J].Food Hydrocolloids,2015,50:16-26.DOI:10.1016/j.foodhyd.2015.04.006.
[3]ADITYA N P,YANG H,KIM S,et al.Fabrication of amorphous curcumin nanosuspensions usingβ-lactoglobulin to enhance solubility,stability,and bioavailability[J].Colloids and Surfaces B:Biointerfaces,2015,127:114-121.DOI:10.1016/j.colsurfb.2015.01.027.
[4]PEREZ A A,SPONTON O E,ANDERMATTEN R B,et al.Biopolymer nanoparticles designed for polyunsaturated fatty acid vehiculization:protein-polysaccharide ratio study[J].Food Chemistry,2015,188:543-550.DOI:10.1016/j.foodchem.2015.05.043.
[5]HE Z Y,CHEN J,MOSER S E.Interaction ofβ-lactoglobulin with(.)-epigallocatechin-3-gallate under different processing conditions of pH and temperature by the fluorescence quenching method[J].European Food Research and Technology,2015,241(3):357-366.DOI:10.1007/s00217-015-2466-2.
[6]WILDE S C,KEPPLER J K,PALANI K,et al.β-Lactoglobulin as nanotransporter for allicin:sensory properties and applicability in food[J].Food Chemistry,2016,199:667-674.DOI:10.1016/j.foodchem.2015.12.055.
[7]左玉.多酚类化合物研究进展[J].粮食与油脂,2013,26(4):6-10.DOI:10.3969/j.issn.1008-9578.2013.04.004.
[8]LESTRINGANT P,GURI A,GU.LSEREN I,et al.Effect of processing on physicochemical characteristics and bioefficacy ofβ-lactoglobulinepigallocatechin-3-gallate complexes[J].Journal of Agricultural and Food Chemistry,2014,62(33):8357-8364.DOI:10.1021/jf5029834.
[9]WU X L,ZHONG X J,LIU M X,et al.Reduced allergenicity ofβ-lactoglobulin in vitro by tea catechins binding[J].Food and Agricultural Immunology,2013,24(3):305-313.DOI:10.1080/09540105.2012.686989.
[10]LI Z,HA J H,ZOU T,et al.Fabrication of coated bovine serum albumin(BSA)-epigallocatechin gallate(EGCG)nanoparticles and their transport across monolayers of human intestinal epithelial Caco-2 cells[J].Food&Function,2014,5(6):1278-1285.DOI:10.1039/c3fo60500k.
[11]ZORILLA R,LIANG L,REMONDETTO G,et al.Interaction of epigallocatechin-3-gallate withβ-lactoglobulin:molecular characterization and biological implication[J].Dairy Science&Technology,2011,91(5):629-644.DOI:10.1007/s13594-011-0036-3.
[12]GOMAA A I,NSONZI F,SEDMAN J,et al.Enhanced unfolding of bovineβ-lactoglobulin structure using microwave treatment:a multi-spectroscopic study[J].Food Biophysics,2016,11(4):1-10.DOI:10.1007/s11483-016-9451-6.
[13]卞蓉霞.微波辅助酶解乳清蛋白的研究[D].无锡:江南大学,2013.
[14]JIA J J,GAO X,HAO M H,et al.Comparison of binding interaction betweenβ-lactoglobulin and three common polyphenols using multispectroscopy and modeling methods[J].Food Chemistry,2017,228:143-151.DOI:10.1016/j.foodchem.2017.01.131.
[15]LAKOWICZ J R.Fluorophores[M]//Principles of fluorescence spectroscopy.US:Springer,1999:63-93.DOI:10.1007/978-1-4757-3061-6_2.
[16]许铭珠.葡萄皮花青素提取物与蛋白质的相互作用及其对色素稳定性的影响[D].无锡:江南大学,2015.
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[21]SURYAWANSHI V D,ANBHULE P V,GORE A H,et al.A spectral deciphering the perturbation of model transporter protein(HSA)by antibacterial pyrimidine derivative:pharmacokinetic and biophysical insights[J].Journal of Photochemistry and Photobiology B:Biology,2013,118:1-8.DOI:10.1016/j.jphotobiol.2012.09.010.
[22]TANG B,HUANG Y M,MA X L,et al.Multispectroscopic and docking studies on the binding of chlorogenic acid isomers to human serum albumin:effects of esteryl position on affinity[J].Food Chemistry,2016,212:434-442.DOI:10.1016/j.foodchem.2016.06.007.
[23]李明.基于乳清蛋白运载和乳化作用提高姜黄素生物利用率的研究[D].哈尔滨:哈尔滨工业大学,2014.
[24]赵焕焦,吕晓玲,王梦姝,等.β-乳球蛋白与黑米花色苷的相互作用[J].食品科学,2017,38(9):85-90.DOI:10.7506/spkx1002-6630-201709014.
[25]RASTEGARI B,KARBALAEI-HEIDARI H R,YOUSEFI R,et al.Interaction of prodigiosin with HSA andβ-LG:spectroscopic and molecular docking studies[J].Bioorganic&Medicinal Chemistry,2016,24(7):1504-1512.DOI:10.1016/j.bmc.2016.02.020.
[26]XIAO J B,SHI J,CAO H,et al.Analysis of binding interaction between puerarin and bovine serum albumin by multi-spectroscopic method[J].Journal of Pharmaceutical and Biomedical Analysis,2007,45(4):609-615.DOI:10.1016/j.jpba.2007.08.032.
[27]SHPIGELMAN A,ISRAELI G,LIVNEY Y D.Thermallyinduced protein-polyphenol co-assemblies:beta lactoglobulinbased nanocomplexes as protective nanovehicles for EGCG[J].Food Hydrocolloids,2010,24(8):735-743.DOI:10.1016/j.foodhyd.2010.03.015.
[28]GHOLAMI S,BORDBAR A K.Exploring binding properties of naringenin with bovineβ-lactoglobulin:a fluorescence,molecular docking and molecular dynamics simulation study[J].Biophysical Chemistry,2014,187:33-42.DOI:10.1016/j.bpc.2014.01.003.
[29]ZHANG L L,WANG Y M,XU M,et al.Galloyl moieties enhance the binding of(.)-epigallocatechin-3-gallate toβ-lactoglobulin:a spectroscopic analysis[J].Food Chemistry,2017,237:39-45.DOI:10.1016/j.foodchem.2017.05.048.
[30]MOHAMMADI F,SAHIHI M,BORDBAR A K.Multispectroscopic and molecular modeling studies on the interaction of two curcuminoids withβ-lactoglobulin[J].Spectrochimica Acta Part A:Molecular&Biomolecular Spectroscopy,2015,140:274-282.DOI:10.1016/j.saa.2014.12.032.
[31]张明,方冰,张录达,等.牛α-乳白蛋白-亚油酸复合物的结构及抗肿瘤活性[J].光谱学与光谱分析,2015,35(9):2609-2612.DOI:10.3964/j.issn.1000-0593(2015)09-2609-04.