24-表油菜素内酯减轻杏果实冷害及与活性氧的代谢关系
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摘要
以新疆赛买提杏为试验原料,选用0.9 mg/L的24-表油菜素内酯(24-epibrassionolide,EBR)在0.05 MPa的压强下对杏果实进行减压渗透处理,以蒸馏水处理作为对照,将处理后的果实取出晾干后在温度为4℃、RH 90%~95%的冷库中贮藏。定期测定杏果实的冷害发病率和冷害指数及过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、超氧阴离子自由基(O_2~-·)的产生速率和过氧化氢(H2O2)的含量。结果表明:浓度为0.9 mg/L的24-表油菜素内酯能有效提高杏果实SOD、APX的活性,减缓杏果实冷藏期间POD和CAT活性的下降,抑制H_2O_2含量和O_2~-·产生速率的增长,显著降低杏果实冷害发病率和冷害指数。说明24-表油菜素内酯处理减轻杏果实冷害与防止果实冷藏期间的氧化伤害,清除自由基的积累密切相关。
Saimaiti apricots sampled from Xinjiang were infiltrated by 0.9 mg/L 24-epibrassinolide(EBR) under the pressure of 0.05 MPa, and the distilled water treatment was used as a control. The treated fruits were taken out, air-dried and stored in a cold room at 4℃ and relative humidity of 90%-95%. Incidence of chilling injury and chilling injury index, the activities of catalase(CAT), ascorbate peroxidase(APX), superoxide dismutase(SOD),peroxidase(POD) and superoxide anion(O_2~-) and the content ofhydrogen peroxide(H_2O_2) of apricot fruits were determined regularly. The results showed that 0.9 mg/L 24-epibrassionolide treatment could increase the activities of SOD and APX, and decreased the activities of POD and CAT in apricot fruit during cold storage. It also inhibited the content of H_2O_2 and the increase of generation rate of O_2~-, and significantly decreased the incidence of chilling injury and chilling injury index of apricot fruit. Results indicated that the 24-epibrassionolide treatment was related to the reduction of chilling injury, prevention of oxidative damage and the accumulation of free radical scavenging during apricot preservation.
Saimaiti apricots sampled from Xinjiang were infiltrated by 0.9 mg/L 24-epibrassinolide(EBR) under the pressure of 0.05 MPa, and the distilled water treatment was used as a control. The treated fruits were taken out, air-dried and stored in a cold room at 4℃ and relative humidity of 90%-95%. Incidence of chilling injury and chilling injury index, the activities of catalase(CAT), ascorbate peroxidase(APX), superoxide dismutase(SOD),peroxidase(POD) and superoxide anion(O_2~-) and the content ofhydrogen peroxide(H_2O_2) of apricot fruits were determined regularly. The results showed that 0.9 mg/L 24-epibrassionolide treatment could increase the activities of SOD and APX, and decreased the activities of POD and CAT in apricot fruit during cold storage. It also inhibited the content of H_2O_2 and the increase of generation rate of O_2~-, and significantly decreased the incidence of chilling injury and chilling injury index of apricot fruit. Results indicated that the 24-epibrassionolide treatment was related to the reduction of chilling injury, prevention of oxidative damage and the accumulation of free radical scavenging during apricot preservation.
引文
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[10]李园园,王莉,周梦洁,等.2,4-表油菜素内酯对草莓果实贮藏品质及抗氧化活性的影响[J].食品科学,2017.;http://kns.cnki.net/kcms/detail/11.2206.TS.20170111.1430.038.html LI Yuan-yuan,WANG Li,ZHOU Meng-jie,et al.Effect of2,4-epibrassionolide on the postharvest quality and antioxidant activity of strawberry fruit[J].Food Science,2017 http://kns.cnki.net/kcms/detail/11.2206.TS.20170111.1430.038.html
[11]Wang Q,Ding T,Gao L P,et al.Effect of brassinolide on chilling injuryof green bell pepper in storage[J].Scientia Horticulturae,2012,144:195-200
[12]Gao H,Kang L N,Gao L P,et al.Effect of 24-epibrassinolide treatment on the metabolism of eggplant fruits in relation to development of pulp browning under chilling stress[J].Journal of Food Science&Technology,2015,52(6):3394-3401
[13]Aghdam M S,Asgari M,Farmani B,et al.Impact of postharvest brassinosteroids treatment on pal activity in ttomato fruit in response to chilling stress[J].Scientia Horticulturae,2012,144:116-120
[14]Gao H,Zhang Z k,Lv X G,et al.Effect of 24-epibrassinolide on chilling injury of peach fruit in relation to phenolic and proline metabolisms[J].Postharvest Biology&Technology,2016,111:390-397
[15]Hu W H,Wu Y,Zeng J Z,et al.Chillinduced iinhibition of photosynthes is was alleviated by24-epibrassinolide pretreatment in cucumber during chilling and subsequent recovery[J].Photosynthetica,2010,48(4):537-544
[16]DONG Li,ZHOU Hong-wei,SONEGO L,et al.Ethylene involvement in the cold storage disorder o-f Flavortop’nectarine[J].Postharvest Biology and Technology,2001,23(2):105-115
[17]ZHOU Biyan,WANG Jihua,Guo Zhenfei,et al.A simple colorimetric method for determination of hy-drogen peroxide in plant tissues[J].Plant Growth Regulation,2006,49:113-118
[18]金昌梅,阚娟,王红梅,等.桃果实成熟软化过程中活性氧代谢的变化[J].扬州大学学报:农业与生命科学版,2006,27(4):86-89JIN Chang-mei,KAN Juan,WANG Hong-mei,et al.Changes of active oxygen metabolism during the ripening of peach fruit[J].Journal of Yangzhou University(Agricultural and Life Science Edition),2006,27(4):86-89
[19]Giannopolitis C N,Ries S K.Superoxide dismutases:I.Occurrence in higher plants[J].Plant Physiology,1977,59(2):309-314
[20]曹建康,姜微波,赵玉梅.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007CAO Jian-kang.JIANG Wei-bo,ZHAO Yu-mei.Experiment guidance of postharvest physiology and biochemistry of fruits and vegetables[M].Beijing:China Light Industry Press,2007
[21]Nakano Y,Asada K.Hydrogen peroxide is scavenged by ascobate specific peroxidase in spinach chl-oroplasts[J].Plant Cell Physiology,1981,22(5):867-880
[22]Wada L,Ou B.Antioxidant Activity and phenolid content of Oregon caneberries[J].Joumal of Agri-cultural Food Chemistry,2002,50(12):3495-3500
[23]Chun OK,Kim DO,Lee CY.Superoxide radical scavenging activity of the major polyphenols in fresh plums[J].Journal of Agricultural and Food Chemistry,2003,51(27):8067-8072
[24]范林林,高丽朴,王清,等.油菜素内酯对豇豆冷害的控制[J].食品工业科技,2016,37(15):339-343FAN Lin-lin,GAO Li-pu,WANG Qing,et al.Effect of treatment with BR on chilling injury of postharvest cowpea[J].Science and Technology of Food Industry,2016,37(15):339-343
[2]马岳岳,李永才,胡培芳,等.采后多胺处理对杏果实黑斑病的控制及贮藏品质的影响[J].食品科学,2017.http://kns.cnki.net/kcms/detail/11.2206.TS.20170323.1438.028.html MA Yue-yue,LI Yong-cai,HU Pei-fang,et al.Effect of postharvest polyamines treatment on black spot disease control and storage quality of apricot fruit[J].Food Science,2017 http://kns.cnki.net/kcms/detail/11.2206.TS.20170323.1438.028.html
[3]杨婷婷,朱璇,向玉洁,等.采收成熟度对杏果实贮藏品质的影响[J].现代食品科技,2015,31(7):277-282YANG Ting-ting,ZHU Xuan,XIANG Yu-jie,et al.Impact of harvest maturity on storage quality of apricot fruits[J].Modern Food Science&Technology,2015,31(7):277-282
[4]侯媛媛,朱璇,王英,等.水杨酸处理对杏果实冷害及活性氧代谢的影响[J].食品科学,2014,35(4):195-199HOU Yuan-yuan,ZHU Xuan,WANG Ying,et al.Effect of salicylic acid treatment on chilling injury and the metabolism of reactive oxygen species in apricot fruits stored at low temperature[J].Food Science,2014,35(4):195-199
[5]Bajguz A,Hayat S.Effects of brassinosteroids on plant responses to environmental stresses[J].Plant physiology&Biochenmistry,2009,47:1-8
[6]Nakashita H,Yasuda M,Nitta T,et al.Brassinosteroid functions in a broad range of disease resistance in tobacco and rice[J].Plant Journal for Cell&Molecular Biology,2003,33(5):887-98
[7]康云艳,杨暹,郭世荣,等.24-表油菜素内酯对低氧胁迫下黄瓜幼苗碳水化合物代谢的影响[J].中国农业科学,2011,44(12):2495-2503KANG Yun-yan,YANG Xian,GUO Shi-rong,et al.Effect of24-epibrassinolide on carbohydrate metabolism and enhancement of tolerance to root-zone hypoxia in cucumber[J].Scientia Agricultura Sinica,2011,44(12):2495-2503
[8]任鸿雁,王莉,马青秀,等.油菜素内酯生物合成途径的研究进展[J].植物学报,2015,50(6):768-778REN Hong-yan,WANG Li,MA Qing-xiu,et al.Research progress of biosynthesis pathway of brassinolide[J].Bulletin of Botany,2015,50(6):768-778
[9]唐如雪,葛永红,李灿婴,等.采后油菜素内酯处理对久保桃果实活性氧代谢的影响[J].保鲜与加工,2016,16(5):5-9TANG Ru-xue,GE Yong-hong,LI Can-ying,et al.Effect of brassinolide treatment on the active oxygen metabolism of postharvest‘Okubao’peach[J].Storage and Process,2016,16(5):5-9
[10]李园园,王莉,周梦洁,等.2,4-表油菜素内酯对草莓果实贮藏品质及抗氧化活性的影响[J].食品科学,2017.;http://kns.cnki.net/kcms/detail/11.2206.TS.20170111.1430.038.html LI Yuan-yuan,WANG Li,ZHOU Meng-jie,et al.Effect of2,4-epibrassionolide on the postharvest quality and antioxidant activity of strawberry fruit[J].Food Science,2017 http://kns.cnki.net/kcms/detail/11.2206.TS.20170111.1430.038.html
[11]Wang Q,Ding T,Gao L P,et al.Effect of brassinolide on chilling injuryof green bell pepper in storage[J].Scientia Horticulturae,2012,144:195-200
[12]Gao H,Kang L N,Gao L P,et al.Effect of 24-epibrassinolide treatment on the metabolism of eggplant fruits in relation to development of pulp browning under chilling stress[J].Journal of Food Science&Technology,2015,52(6):3394-3401
[13]Aghdam M S,Asgari M,Farmani B,et al.Impact of postharvest brassinosteroids treatment on pal activity in ttomato fruit in response to chilling stress[J].Scientia Horticulturae,2012,144:116-120
[14]Gao H,Zhang Z k,Lv X G,et al.Effect of 24-epibrassinolide on chilling injury of peach fruit in relation to phenolic and proline metabolisms[J].Postharvest Biology&Technology,2016,111:390-397
[15]Hu W H,Wu Y,Zeng J Z,et al.Chillinduced iinhibition of photosynthes is was alleviated by24-epibrassinolide pretreatment in cucumber during chilling and subsequent recovery[J].Photosynthetica,2010,48(4):537-544
[16]DONG Li,ZHOU Hong-wei,SONEGO L,et al.Ethylene involvement in the cold storage disorder o-f Flavortop’nectarine[J].Postharvest Biology and Technology,2001,23(2):105-115
[17]ZHOU Biyan,WANG Jihua,Guo Zhenfei,et al.A simple colorimetric method for determination of hy-drogen peroxide in plant tissues[J].Plant Growth Regulation,2006,49:113-118
[18]金昌梅,阚娟,王红梅,等.桃果实成熟软化过程中活性氧代谢的变化[J].扬州大学学报:农业与生命科学版,2006,27(4):86-89JIN Chang-mei,KAN Juan,WANG Hong-mei,et al.Changes of active oxygen metabolism during the ripening of peach fruit[J].Journal of Yangzhou University(Agricultural and Life Science Edition),2006,27(4):86-89
[19]Giannopolitis C N,Ries S K.Superoxide dismutases:I.Occurrence in higher plants[J].Plant Physiology,1977,59(2):309-314
[20]曹建康,姜微波,赵玉梅.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007CAO Jian-kang.JIANG Wei-bo,ZHAO Yu-mei.Experiment guidance of postharvest physiology and biochemistry of fruits and vegetables[M].Beijing:China Light Industry Press,2007
[21]Nakano Y,Asada K.Hydrogen peroxide is scavenged by ascobate specific peroxidase in spinach chl-oroplasts[J].Plant Cell Physiology,1981,22(5):867-880
[22]Wada L,Ou B.Antioxidant Activity and phenolid content of Oregon caneberries[J].Joumal of Agri-cultural Food Chemistry,2002,50(12):3495-3500
[23]Chun OK,Kim DO,Lee CY.Superoxide radical scavenging activity of the major polyphenols in fresh plums[J].Journal of Agricultural and Food Chemistry,2003,51(27):8067-8072
[24]范林林,高丽朴,王清,等.油菜素内酯对豇豆冷害的控制[J].食品工业科技,2016,37(15):339-343FAN Lin-lin,GAO Li-pu,WANG Qing,et al.Effect of treatment with BR on chilling injury of postharvest cowpea[J].Science and Technology of Food Industry,2016,37(15):339-343