氟铃脲对韭菜迟眼蕈蚊不同虫态的致毒特点及毒力
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摘要
为明确氟铃脲对韭菜迟眼蕈蚊不同虫态的致毒特点及毒力,采用胃毒触杀联合毒力法、药液定量滴加法及药膜法分别对韭菜迟眼蕈蚊卵、幼虫、蛹、成虫进行了生物测定。结果表明,经氟铃脲处理的0.5~1、1.5~2和2.5~3日龄韭菜迟眼蕈蚊卵至孵化24 h后,LC_(50)依次为2.27、1.60和0.64 mg/L,对1.5~3日龄卵的毒力高于对0.5~1日龄卵;经氟铃脲处理的2日龄卵、2龄幼虫、4龄幼虫、1日龄和3日龄蛹至羽化时LC_(50)分别为0.06、0.30、0.34、24.05和27.42 mg/L,毒力程度随韭菜迟眼蕈蚊发育而逐步下降;对蛹和成虫毒力较低,100 mg/L氟铃脲染毒1、3日龄蛹的羽化率分别高于10%和20%;药膜法100、1 000 mg/L氟铃脲处理成虫24 h,校正死亡率低于20%。研究表明,氟铃脲对韭菜迟眼蕈蚊各虫态的生长发育均有不利影响,抑制卵的孵化并导致初孵幼虫存活率降低;对幼虫毒杀作用较慢,对蛹及成虫阶段毒力较低。
To figure out the toxic characters and toxicity of hexaflumuron against different developmental stage Bradysia odoriphaga Yang et Zhang( Diptera: Sciaridae), bioassays were conducted using stomach-contact combination toxicity methods,the dripping ration liquid methods and the dry film residue method under laboratory conditions to determine the toxicity against eggs,larvae,pupae and adults of B.odoriphaga,respectively. The results showed that the LC_(50) values of 0. 5- 1,1. 5- 2 and 2. 5- 3 dayold eggs treated by hexaflumuron were 2. 27,1. 60 and 0. 64 mg / L,respectively,when observed until 24 h after new larva hatching. It was more toxic to the 1. 5- 3 day-old eggs than to the 0. 5- 1 day-old eggs. The LC_(50) values of 2 day-old eggs,2nd instar larvae,4th instar larvae,1 day-old pupae and 3 dayold pupae of B. odoriphaga treated by hexaflumuron were 0. 06,0. 30,0. 34,24. 05 and 27. 42 mg / L,respectively. The toxic level decreased progressively with the developmental process of B. odoriphaga.The toxicity to pupae and adults were lower than to larvae. At the dose of 100 mg / L,the emergence rates of 1 day-and 3 day-old pupae were above 10% and 20%,respectively. Besides,the corrected mortality rate of adults treated by dry film residue method was less than 20% after 24 h at the dose of 100 and1 000 mg / L. This study indicated that hexaflumuron had negative impacts on each developmental stages of B. odoriphaga,which could inhibit the hatching rate of eggs and decrease the survival rate of newlyhatched larvae. The toxic speed of hexaflumuron against larvae was slow,and lower toxicity was shown during pupal and adult periods.
To figure out the toxic characters and toxicity of hexaflumuron against different developmental stage Bradysia odoriphaga Yang et Zhang( Diptera: Sciaridae), bioassays were conducted using stomach-contact combination toxicity methods,the dripping ration liquid methods and the dry film residue method under laboratory conditions to determine the toxicity against eggs,larvae,pupae and adults of B.odoriphaga,respectively. The results showed that the LC_(50) values of 0. 5- 1,1. 5- 2 and 2. 5- 3 dayold eggs treated by hexaflumuron were 2. 27,1. 60 and 0. 64 mg / L,respectively,when observed until 24 h after new larva hatching. It was more toxic to the 1. 5- 3 day-old eggs than to the 0. 5- 1 day-old eggs. The LC_(50) values of 2 day-old eggs,2nd instar larvae,4th instar larvae,1 day-old pupae and 3 dayold pupae of B. odoriphaga treated by hexaflumuron were 0. 06,0. 30,0. 34,24. 05 and 27. 42 mg / L,respectively. The toxic level decreased progressively with the developmental process of B. odoriphaga.The toxicity to pupae and adults were lower than to larvae. At the dose of 100 mg / L,the emergence rates of 1 day-and 3 day-old pupae were above 10% and 20%,respectively. Besides,the corrected mortality rate of adults treated by dry film residue method was less than 20% after 24 h at the dose of 100 and1 000 mg / L. This study indicated that hexaflumuron had negative impacts on each developmental stages of B. odoriphaga,which could inhibit the hatching rate of eggs and decrease the survival rate of newlyhatched larvae. The toxic speed of hexaflumuron against larvae was slow,and lower toxicity was shown during pupal and adult periods.
引文
Abo-Elghar GE,El-Sheikh AE,El-Sayed FM,El-Maghraby HM,El-Zun HM.2004.Persistance and residual activity of an organophosphate,pirimiphos-methyl,and three IGRs,hexaflumuron,teflubenzuron and pyriproxyfen,against the cowpea weevil,Callosobruchus maculates(Coleoptera:Bruchidae).Pest Management Science,60(1):95-102
Ashida M,Brey PT.1995.Role of the integument in insect defense:pro-phenol oxidase cascade in the cuticular matrix.Proceedings of the National Academy of Sciences of the United States of A-merica,92(23):10698-10702
Chen P,Gong HF.1989.The influence of a chitin synthesis inhibitor on the tracheal system formation in the embryo of Asian corn borer.Acta Entomologica Sinica,32(2):144-148(in Chinese)[陈沛,弓惠芬.1989.灭幼脲对亚洲玉米螟胚胎气管系统形成的影响.昆虫学报,32(2):144-148]
Chen P,Gong HF,Wang R.1982.Determination on the activity of the chitin synthesis inhibitors and the juvenile hormone analogues against eggs of the Asiatic corn borer Ostrinia furnacalis Guenee.Journal of Plant Protection,9(1):35-40(in Chinese)[陈沛,弓惠芬,王瑞.1982.灭幼脲类及昆虫保幼激素类似物对玉米螟卵的生物活性.植物保护学报,9(1):35-40]
Dang ZH,Dong JZ,Gao ZL,Jia HM,Zhang KJ.2001.Biology and injury of Bradysia odoriphaga on leek in different types of cultivation.Journal of Agricultural University of Hebei,24(4):65-68(in Chinese)[党志红,董建臻,高占林,贾海民,张克锦.2001.不同种植方式下韭菜迟眼蕈蚊发生为害规律的研究.河北农业大学学报,24(4):65-68]
Feng HQ,Zheng FQ.1987.Studies on the occurrence and control of Bradysia odoriphaga Yang et Zhang.Journal of Shandong Agricultural University,18(1):71-80(in Chinese)[冯惠琴,郑方强.1987.韭蛆发生规律及防治研究.山东农业大学学报,18(1):71-80]
Jiao YY,Liu YJ,Qiu XC,Liu H.2010.Effects of hexaflumuron on phenoloxidase activity in Spodoptera litura(Fabricius)(Lepidoptera:Noctuidae).Acta Entomologica Sinica,53(5):517-524(in Chinese)[焦艳艳,刘永杰,邱秀翠,刘辉.2010.氟铃脲对斜纹夜蛾酚氧化酶活性的影响.昆虫学报,53(5):517-524]
Kellouche A,Soltani N.2006.Impact of hexaflumuron,a chitin synthesis inhibitor,on growth,development and reproductive performance of the progeny in Callosobruchus maculatus after adult treatments.African Journal of Agricultural Research,1(3):57-64
Mahmoudvand M,Sheikhi-Garjan A,Abbasipour H.2011.Ovicidal effect of some insecticides on the diamondback moth,Plutella xylostella(L.)(Lepidoptera:Y ponomeutidae).Chilean Journal of A gricultural Research,71(2):226-230
Mei ZX,Wu QJ,Zhang YJ,Hua L.2004.Life tables of the laboratory population of Bradysia odoriphaga Yang et Zhang(Diptera:Mycetophilidae)at different temperatures.Acta Entomologica Sinica,47(2):219-222(in Chinese)[梅增霞,吴青君,张友军,花蕾.2004.韭菜迟眼蕈蚊在不同温度下的实验种群生命表.昆虫学报,47(2):219-222]
Mendel Z,Blumberg D,Ishaaya I.1994.Effects of some insect growth regulators on natural enemies of scale insects(Hom.:Coccoidea).Bio Control,39(2):199-209
Mirhaghparast SK,Zibaee A.2013.Effects of hexaflumuron and pyriproxyfen on the purified phenoloxidase of Chilo suppressalis Walker(Lepidoptera:Crambidae).Archives of Phytopathology and Plant Protection,46(15):1775-1784
Mu W,Ding Z,He MH,Guo H,Liu F.2002.Bioassay technique of Bradysia odoriphaga and control to larvae and adults by insecticides.Acta Agriculturae Boreali-Sinica,17(S1):12-16(in Chinese)[慕卫,丁中,何茂华,郭红,刘峰.2002.韭菜迟眼蕈蚊的生测方法及防治药剂研究.华北农学报,17(S1):12-16]
Mu W,Liu F,Jia ZM,He MH,Xiang GF.2003.A simple and convenient rearing technique for Bradysia odoriphaga.Entomological Journal of East China,12(2):87-89(in Chinese)[慕卫,刘峰,贾忠明,何茂华,相冠锋.2003.韭菜迟眼蕈蚊简便人工饲养技术.华东昆虫学报,12(2):87-89]
Ren JC,Ma Y,Zhang ZB.1988.Microscopical observation on the histopathological changes of cuticle induced by diflubenzuronin two insect larvae.Acta Entomologica Sinica,31(4):366-370(in Chinese)[任久长,马燕,张宗炳.1988.灭幼脲引起两种幼虫表皮组织病变的显微观察.昆虫学报,31(4):366-370]
Song ZM,Xue M,Wang HT.2007.Effects of six insect growth regulators on the growth,development and reproduction of Delia antique(Meigen)(Diptera:Anthomyiidae).Acta Entomologica Sinica,50(8):775-781(in Chinese)[宋增明,薛明,王洪涛.2007.六种昆虫生长调节剂对葱蝇生长发育和繁殖力的影响.昆虫学报,50(8):775-781]
Vasuki V.1992.The effects of sublethal doses of hexaflumuron on the feeding behavior of mosquitoes(Diptera:Culicidae).Bulletin of Entomological Research,82(4):535-538
Wang S,Allan RD,Skerritt JH,Kennedy IR.1998.Development of a class-specific competitive ELISA for the benzoylphenyl urea insecticides.Journal of Agricultural and Food Chemistry,46(8):3330-3338
Xue M,Wang YX.2002.Study on the insecticides of controlling Bradysia odoriphaga and unpolluting vegetable.Pesticides,41(5):29-31(in Chinese)[薛明,王永显.2002.韭菜迟眼蕈蚊无公害治理药剂的研究.农药,41(5):29-31]
Xue M,Yuan L,Xu ML.2002.The olfactory response of adults to volatiles and compare of toxicity of different insecticides to the adults and larvae of Bradysia odoriphaga.Chinese Journal of Pesticide Science,4(2):50-56(in Chinese)[薛明,袁林,徐曼琳.2002.韭菜迟眼蕈蚊成虫对挥发性物质的嗅觉反应及不同杀虫剂的毒力比较.农药学学报,4(2):50-56]
Yan SJ,Lin J,Bi FC,Yang LJ,Chen YP.2003.Test of the insecticidal activity of novel benzoylphenyl urea compounds.Journal of Yunnan University,25(5):438-441(in Chinese)[严胜骄,林军,毕富春,杨丽娟,陈云平.2003.几种新型苯甲酰基脲类化合物的杀虫活性研究.云南大学学报(自然科学版),25(5):438-441]
Zhang P,Chen CY,Li H,Liu F,Mu W.2014.Selective toxicity of seven neonicotinoid insecticides to fungus gnat Bradysia odoriphaga and earthworm Eisenia foetida.Journal of Plant Protection,41(1):79-86(in Chinese)[张鹏,陈澄宇,李慧,刘峰,慕卫.2014.七种新烟碱类杀虫剂对韭菜迟眼蕈蚊幼虫及蚯蚓的选择毒力.植物保护学报,41(1):79-86]
Zhuang ZX,Han SX,Zhang CX.2003.Study on the activity and application techniques of dichlorbenzuron against larvae of Bradysia odoriphaga.Pesticide Science and Administration,24(4):19-21(in Chinese)[庄占兴,韩书霞,张春学.2003.灭幼脲对韭菜迟眼蕈蚊的活性及其应用技术研究.农药科学与管理,24(4):19-21]
Ashida M,Brey PT.1995.Role of the integument in insect defense:pro-phenol oxidase cascade in the cuticular matrix.Proceedings of the National Academy of Sciences of the United States of A-merica,92(23):10698-10702
Chen P,Gong HF.1989.The influence of a chitin synthesis inhibitor on the tracheal system formation in the embryo of Asian corn borer.Acta Entomologica Sinica,32(2):144-148(in Chinese)[陈沛,弓惠芬.1989.灭幼脲对亚洲玉米螟胚胎气管系统形成的影响.昆虫学报,32(2):144-148]
Chen P,Gong HF,Wang R.1982.Determination on the activity of the chitin synthesis inhibitors and the juvenile hormone analogues against eggs of the Asiatic corn borer Ostrinia furnacalis Guenee.Journal of Plant Protection,9(1):35-40(in Chinese)[陈沛,弓惠芬,王瑞.1982.灭幼脲类及昆虫保幼激素类似物对玉米螟卵的生物活性.植物保护学报,9(1):35-40]
Dang ZH,Dong JZ,Gao ZL,Jia HM,Zhang KJ.2001.Biology and injury of Bradysia odoriphaga on leek in different types of cultivation.Journal of Agricultural University of Hebei,24(4):65-68(in Chinese)[党志红,董建臻,高占林,贾海民,张克锦.2001.不同种植方式下韭菜迟眼蕈蚊发生为害规律的研究.河北农业大学学报,24(4):65-68]
Feng HQ,Zheng FQ.1987.Studies on the occurrence and control of Bradysia odoriphaga Yang et Zhang.Journal of Shandong Agricultural University,18(1):71-80(in Chinese)[冯惠琴,郑方强.1987.韭蛆发生规律及防治研究.山东农业大学学报,18(1):71-80]
Jiao YY,Liu YJ,Qiu XC,Liu H.2010.Effects of hexaflumuron on phenoloxidase activity in Spodoptera litura(Fabricius)(Lepidoptera:Noctuidae).Acta Entomologica Sinica,53(5):517-524(in Chinese)[焦艳艳,刘永杰,邱秀翠,刘辉.2010.氟铃脲对斜纹夜蛾酚氧化酶活性的影响.昆虫学报,53(5):517-524]
Kellouche A,Soltani N.2006.Impact of hexaflumuron,a chitin synthesis inhibitor,on growth,development and reproductive performance of the progeny in Callosobruchus maculatus after adult treatments.African Journal of Agricultural Research,1(3):57-64
Mahmoudvand M,Sheikhi-Garjan A,Abbasipour H.2011.Ovicidal effect of some insecticides on the diamondback moth,Plutella xylostella(L.)(Lepidoptera:Y ponomeutidae).Chilean Journal of A gricultural Research,71(2):226-230
Mei ZX,Wu QJ,Zhang YJ,Hua L.2004.Life tables of the laboratory population of Bradysia odoriphaga Yang et Zhang(Diptera:Mycetophilidae)at different temperatures.Acta Entomologica Sinica,47(2):219-222(in Chinese)[梅增霞,吴青君,张友军,花蕾.2004.韭菜迟眼蕈蚊在不同温度下的实验种群生命表.昆虫学报,47(2):219-222]
Mendel Z,Blumberg D,Ishaaya I.1994.Effects of some insect growth regulators on natural enemies of scale insects(Hom.:Coccoidea).Bio Control,39(2):199-209
Mirhaghparast SK,Zibaee A.2013.Effects of hexaflumuron and pyriproxyfen on the purified phenoloxidase of Chilo suppressalis Walker(Lepidoptera:Crambidae).Archives of Phytopathology and Plant Protection,46(15):1775-1784
Mu W,Ding Z,He MH,Guo H,Liu F.2002.Bioassay technique of Bradysia odoriphaga and control to larvae and adults by insecticides.Acta Agriculturae Boreali-Sinica,17(S1):12-16(in Chinese)[慕卫,丁中,何茂华,郭红,刘峰.2002.韭菜迟眼蕈蚊的生测方法及防治药剂研究.华北农学报,17(S1):12-16]
Mu W,Liu F,Jia ZM,He MH,Xiang GF.2003.A simple and convenient rearing technique for Bradysia odoriphaga.Entomological Journal of East China,12(2):87-89(in Chinese)[慕卫,刘峰,贾忠明,何茂华,相冠锋.2003.韭菜迟眼蕈蚊简便人工饲养技术.华东昆虫学报,12(2):87-89]
Ren JC,Ma Y,Zhang ZB.1988.Microscopical observation on the histopathological changes of cuticle induced by diflubenzuronin two insect larvae.Acta Entomologica Sinica,31(4):366-370(in Chinese)[任久长,马燕,张宗炳.1988.灭幼脲引起两种幼虫表皮组织病变的显微观察.昆虫学报,31(4):366-370]
Song ZM,Xue M,Wang HT.2007.Effects of six insect growth regulators on the growth,development and reproduction of Delia antique(Meigen)(Diptera:Anthomyiidae).Acta Entomologica Sinica,50(8):775-781(in Chinese)[宋增明,薛明,王洪涛.2007.六种昆虫生长调节剂对葱蝇生长发育和繁殖力的影响.昆虫学报,50(8):775-781]
Vasuki V.1992.The effects of sublethal doses of hexaflumuron on the feeding behavior of mosquitoes(Diptera:Culicidae).Bulletin of Entomological Research,82(4):535-538
Wang S,Allan RD,Skerritt JH,Kennedy IR.1998.Development of a class-specific competitive ELISA for the benzoylphenyl urea insecticides.Journal of Agricultural and Food Chemistry,46(8):3330-3338
Xue M,Wang YX.2002.Study on the insecticides of controlling Bradysia odoriphaga and unpolluting vegetable.Pesticides,41(5):29-31(in Chinese)[薛明,王永显.2002.韭菜迟眼蕈蚊无公害治理药剂的研究.农药,41(5):29-31]
Xue M,Yuan L,Xu ML.2002.The olfactory response of adults to volatiles and compare of toxicity of different insecticides to the adults and larvae of Bradysia odoriphaga.Chinese Journal of Pesticide Science,4(2):50-56(in Chinese)[薛明,袁林,徐曼琳.2002.韭菜迟眼蕈蚊成虫对挥发性物质的嗅觉反应及不同杀虫剂的毒力比较.农药学学报,4(2):50-56]
Yan SJ,Lin J,Bi FC,Yang LJ,Chen YP.2003.Test of the insecticidal activity of novel benzoylphenyl urea compounds.Journal of Yunnan University,25(5):438-441(in Chinese)[严胜骄,林军,毕富春,杨丽娟,陈云平.2003.几种新型苯甲酰基脲类化合物的杀虫活性研究.云南大学学报(自然科学版),25(5):438-441]
Zhang P,Chen CY,Li H,Liu F,Mu W.2014.Selective toxicity of seven neonicotinoid insecticides to fungus gnat Bradysia odoriphaga and earthworm Eisenia foetida.Journal of Plant Protection,41(1):79-86(in Chinese)[张鹏,陈澄宇,李慧,刘峰,慕卫.2014.七种新烟碱类杀虫剂对韭菜迟眼蕈蚊幼虫及蚯蚓的选择毒力.植物保护学报,41(1):79-86]
Zhuang ZX,Han SX,Zhang CX.2003.Study on the activity and application techniques of dichlorbenzuron against larvae of Bradysia odoriphaga.Pesticide Science and Administration,24(4):19-21(in Chinese)[庄占兴,韩书霞,张春学.2003.灭幼脲对韭菜迟眼蕈蚊的活性及其应用技术研究.农药科学与管理,24(4):19-21]