生物降解芳香族化合物的分子检测技术研究进展
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摘要
编码环羟基化加氧酶的基因常作为评价环境中微生物降解芳香族化合物能力的分子标记.近年宏基因组学技术的出现极大地加速了对环境中功能基因及功能类群的研究.本文总结了目前关于芳香族化合物环羟基化加氧酶基因的数据库,介绍了微生物降解芳香族化合物(苯系物、萘及其它多环芳烃)过程中发挥重要作用的各类功能基因,总结了环境检测中使用的分子引物,并综述了它们在各类复杂环境样本中的检测应用,此外对使用宏基因组技术来研究微生物在环境中降解芳香族化合物的能力进行了总结与展望.
Microbial genes encoding aromatic ring-hydroxylating oxygenase(ARHOs) have been selected as molecular markers for evaluating the potential of microbial degradation ability in environment. The development of culture-independent metagenomic technology has greatly accelerated the research of functional genes and functional groups on aromatic hydrocarbon degradation in recent years. This article summarized the ARHOs related databases, introduced various functional genes and corresponding primers applied to environment samples in microbial degradation of different types of aromatic hydrocarbons(benzene series, naphthalene as well as other polycyclic aromatic hydrocarbons). It also reviewed the applications of this technology in various complex environment samples. Furthermore, the future development of microbial degradation of aromatic compounds using metagenomics technologies was prospected.
Microbial genes encoding aromatic ring-hydroxylating oxygenase(ARHOs) have been selected as molecular markers for evaluating the potential of microbial degradation ability in environment. The development of culture-independent metagenomic technology has greatly accelerated the research of functional genes and functional groups on aromatic hydrocarbon degradation in recent years. This article summarized the ARHOs related databases, introduced various functional genes and corresponding primers applied to environment samples in microbial degradation of different types of aromatic hydrocarbons(benzene series, naphthalene as well as other polycyclic aromatic hydrocarbons). It also reviewed the applications of this technology in various complex environment samples. Furthermore, the future development of microbial degradation of aromatic compounds using metagenomics technologies was prospected.
引文
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[23]Finn R D,Coggill P,Eberhardt R Y,et al.The Pfam protein families database:towards a more sustainable future[J].Nucleic Acids Research,2016,44(D1):D279-D285.
[24]Kimura N,Kamagata Y.Impact of dibenzofuran/dibenzo-p-dioxin amendment on bacterial community from forest soil and ringhydroxylating dioxygenase gene populations[J].Applied Microbiology and Biotechnology,2009,84(2):365-373.
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[28]He Z L,Deng Y,Van Nostrand J D,et al.GeoChip 3.0as a high-throughput tool for analyzing microbial community composition,structure and functional activity[J].Isme Journal,2010,4(9):1167-1179.
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[30]Rhee S K,Liu X D,Wu L Y,et al.Detection of genes involved in biodegradation and biotransformation in microbial communities by using 50-mer oligonucleotide microarrays[J].Applied and Environmental Microbiology,2004,70(7):4303-4317.
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[32]Nyyssonen M,Piskonen R,Itavaara M.Monitoring aromatic hydrocarbon biodegradation by functional marker genes[J].Environmental Pollution,2008,154(2):192-202.
[33]Piskonen R,Nyyssonen M,Itavaara M.Evaluating the biodegradation of aromatic hydrocarbons by monitoring of several functional genes[J].Biodegradation,2008,19(6):883-895.
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