黄芪多糖改善猪瘟混感母猪免疫抑制效果
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摘要
本试验旨在探讨以黄芪多糖((Astragalus polysaccharide,APS)为免疫增强剂改善猪瘟混感母猪免疫抑制效果。以24头猪瘟(Classical Swine fever,CSF)混感带毒母猪随机分成4个试验组。各组分别于免疫后第20 d、第35 d、第50 d,进行采血和相关指标的测定。结果显示:APS能延长猪瘟抗体效价的衰减时间,显著增加外周血T淋巴细胞数、提高CD4+/CD8+比值,改善产仔性能。表明APS对特异性免疫和非特异性免疫均有促进作用。
To investigate the improving effects o f immunopotentiator astragalus polysaccharide(APS) on the classical swine fever virus(CSFV) poly-infection induced immunosuppression in sows. Sows infected CSFV were randomly divided into 4 groups. The antigens of piglets were detected after 20 d, 35 d, 50 d immunization, respectively.Our results revealed that the decay time of antigen in the APS treated piglets was significantly longer than that in the control group(p < 0.01). Numbers of T lymphocyte and the ratio of CD4 +/CD8 + in the peripheral blood were also increased significantly(p < 0.01). Collectively, these results demonstrate that APS could be used as a good immunopotentiator on the specific immunity and non-specific immunity.
To investigate the improving effects o f immunopotentiator astragalus polysaccharide(APS) on the classical swine fever virus(CSFV) poly-infection induced immunosuppression in sows. Sows infected CSFV were randomly divided into 4 groups. The antigens of piglets were detected after 20 d, 35 d, 50 d immunization, respectively.Our results revealed that the decay time of antigen in the APS treated piglets was significantly longer than that in the control group(p < 0.01). Numbers of T lymphocyte and the ratio of CD4 +/CD8 + in the peripheral blood were also increased significantly(p < 0.01). Collectively, these results demonstrate that APS could be used as a good immunopotentiator on the specific immunity and non-specific immunity.
引文
[1]赵娟,刘凤华,邱河辉,等.黄芪多糖对PCV2阳性猪外周血免疫细胞及相关细胞因子的影响[J].中国兽医杂志,2010,46(3):3-5.
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[14]姚秀娟,王米,江善祥.黄芪多糖药理作用及在动物生产中的应用研究进展[J].饲料工业,2009,30(18):1-3.
[15]杨秀女,孟艳,郑丽丽.黄芪多糖粉对兔瘟疫苗免疫效果及增重的影响[J].养殖与饲料,2011(3):3-4.
[16]刘永录,马霞,王长林.黄芪多糖活性组分对鸡脏淋巴细胞功能的影响[J].中国兽医杂志,2015,51(9):60-63.
[17]王美君,陈静,孙圣福,等.PRRSV持续性感染猪群抗体动态观察及对猪瘟疫苗免疫的影响[J].中国兽医学报,2011,31(5):630-632.
[18]庄金秋,梅建国,沈志强.黄芪多糖的生物学功能及其在养猪生产中的应用研究进展[C].中国畜牧兽医学会兽医公共卫生学分会.第三次学术研讨会,中国广州2012:43-52.
[2]丘惠琛.猪瘟的持续感染的根源在于带毒母猪[J].养猪,2004(3):29-30.
[3]马超英,李宗文.猪瘟病毒和猪繁殖与呼吸综合征病毒双重RT-PCR检测方法的建立[J].动物医学进展,2013,34(9):71-75.
[4]花象柏.我国当前猪病免疫防制关键问题探讨[J].江西养猪,2010,6:1-4.
[5]Ma X Q,Shi Q,Duan J A,et al.Chemical Analysis of Radix Astragali(Huangqi)in China:A Comparison with Its Adultera nts and Seasonal Variations.J Agric Food Chem.,2002,50(17):4861-4866.
[6]田晓丽.黄芪多糖增强动物免疫力的研究概况[J].国外畜牧学(猪与禽),2009(3):66-68.
[7]黄德尚,郭爱珍.黄芪多糖佐剂对猪瘟疫苗免疫效果的影响[J].中国畜牧兽医,2011,38(11):50-52.
[8]何文涓,袁志坚,何晓升.黄芪多糖的药理作用研究进展[J].中国生化药物杂志,2012,33(5):692-694.
[9]曹录秀,陈磊,盛艳梅.黄芪组分配伍对脾淋巴细胞免疫活性的影响[J].时珍国医国药,2014,25(4):830-832.
[10]杨汉春.动物免疫学(第二版)[M].北京:中国农业大学出版社,2003
[11]帅起义,赵俊龙,叶培根.中药免疫增强剂(维康)对母猪免疫应答及生产性能的影响[J].湖南农业科学,2011(11):134-136.
[12]Damian Knecht,Kamil Duziński.The Effect of Parity and Date of Service on the Reproductive Performance of Polish Large White×Polish Landrace(PLW×PL)Crossbred sows.Annals of Animal Science.2014,1.
[13]刘保光,吴华,徐利纳.黄芪多糖的药理学功效及在兽医临床的应用[J].广东饲料,2010,10(6):30-32.
[14]姚秀娟,王米,江善祥.黄芪多糖药理作用及在动物生产中的应用研究进展[J].饲料工业,2009,30(18):1-3.
[15]杨秀女,孟艳,郑丽丽.黄芪多糖粉对兔瘟疫苗免疫效果及增重的影响[J].养殖与饲料,2011(3):3-4.
[16]刘永录,马霞,王长林.黄芪多糖活性组分对鸡脏淋巴细胞功能的影响[J].中国兽医杂志,2015,51(9):60-63.
[17]王美君,陈静,孙圣福,等.PRRSV持续性感染猪群抗体动态观察及对猪瘟疫苗免疫的影响[J].中国兽医学报,2011,31(5):630-632.
[18]庄金秋,梅建国,沈志强.黄芪多糖的生物学功能及其在养猪生产中的应用研究进展[C].中国畜牧兽医学会兽医公共卫生学分会.第三次学术研讨会,中国广州2012:43-52.