补加碳源对采油菌株ZY-1生长及发酵液表面活性的影响
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摘要
该试验选取一株分离自辽河油田原油的菌株ZY-1,分别补加培养基碳源(木糖、淀粉、葡萄糖),于60℃静置培养7 d,通过测定发酵液的菌体密度、表面张力、乳化活性以及石蜡的残留量,考察菌株ZY-1利用上述碳源的生长以及发酵液表面活性情况。结果表明,菌株ZY-1可利用木糖生长,并代谢产生物表面活性剂。补加木糖能明显促进细胞生长,减慢对石蜡的利用,使发酵液表面张力降至49.5 m N/m,发酵液的乳化活性提高至61.3%。补加葡萄糖或淀粉虽不能明显促进细胞生长,但能明显减少石蜡消耗,都能将发酵液表面活性提高至63.3%。木糖、葡萄糖和淀粉都能减少石蜡的消耗,木糖效果更明显。
Strain ZY-1 isolated from Liaohe crude oil for enhancing oil recovery was cultivated at 60℃ for 7 d in the media with xylose, starch and glucose as carbon sources. The growth of strain ZY-1 and surface activity of strain fermentation broth were evaluated by measuring the cell density,surface tension, emulsifying activity and the residues of paraffin. The results showed that the strain ZY-1 could utilize the tested carbon sources for the growth of cells and product biosurfactants by metabolism. The mixed carbon source of paraffin and xylose could obviously improve the growth of cells, slow down the utilization of paraffin, reduce surface tension of fermentation broth to 49.5 m N/m, and increase the emulsification activity of the fermentation broth to 61.3%. Xylose could significantly reduce the consumption of paraffin. The mixed carbon source of paraffin and glucose or starch could not obviously promote cell growth, but obviously reduce the paraffin consumption and enhance the surface activity of fermentation broth to63.3%. Xylose, glucose and starch could reduce the consumption of paraffin, xylose was more effective.
Strain ZY-1 isolated from Liaohe crude oil for enhancing oil recovery was cultivated at 60℃ for 7 d in the media with xylose, starch and glucose as carbon sources. The growth of strain ZY-1 and surface activity of strain fermentation broth were evaluated by measuring the cell density,surface tension, emulsifying activity and the residues of paraffin. The results showed that the strain ZY-1 could utilize the tested carbon sources for the growth of cells and product biosurfactants by metabolism. The mixed carbon source of paraffin and xylose could obviously improve the growth of cells, slow down the utilization of paraffin, reduce surface tension of fermentation broth to 49.5 m N/m, and increase the emulsification activity of the fermentation broth to 61.3%. Xylose could significantly reduce the consumption of paraffin. The mixed carbon source of paraffin and glucose or starch could not obviously promote cell growth, but obviously reduce the paraffin consumption and enhance the surface activity of fermentation broth to63.3%. Xylose, glucose and starch could reduce the consumption of paraffin, xylose was more effective.
引文
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[17]李牧,杨红,刘思贤.微生物表面活性剂在稠油乳化降粘中的实验研究[J].特种油气藏,1999(4):53-58,67.
[18]曹娟,徐志辉,李凌之.生物表面活性剂的石油降解菌Acinetobacter BHSN的研究[J].生态与农村环境学报,2009,25(1):73-78.
[19]金佩强,王琪,杨克远.在MEOR过程中产生生物表面活性剂提高伊朗油藏采收率的试验方法[J].国外油油田工程,2005(10):10-14,20.
[20] MOHAMAD FULAZZAKY, DEA INDRIANI ASTUTI. Laboratory simulation of microbial enhanced oil recovery using Geobacillus toebii R-32639 isolated from the Handil reservoir[J]. RSC Adv, 2015, 5:3908-3916.
[2]王鑫.微生物采油技术提高采收率机理及影响因素分析[J].云南化工,2018(6):87-88.
[3]冯云,段传慧,林军章,等.中高温油藏内源微生物厌氧激活[J].生物加工过程,2016(3):12-16.
[4] ROLING W F, HEAD I M, LAMER S R. The microbiology of hydrocarbon degradation in subsurface petroleum reservoirs and prospects[J]. Res Microbiol, 2003, 154(5):321-328.
[5]李清心,康从宝,林建强,等.芽孢杆菌L-32发酵条件优化及提高原油采收率实验研究[J].石油大学学报:自然科学版,2004(3):52-55,140.
[6]包木太,袁书文,宋智勇,等.不同碳源激活剂对胜利油田中一区油藏内源菌激活效果评价[J].化工学报,2011,62(3):786-791.
[7]于海威.驱油菌SF67的室内性能评价实验研究[J].中国石油和化工标准与质量,2012,32(4):50.
[8] ZHANG G Y, MA F, LIANG Y B, et al. Domain and theory-technology progress of global deep oil&gas exploration[J]. Acta Petrolei Sinica,2015, 36(9):1161-1163.
[9] BANAT I M, MAKKAR R S, CAMEETRAS. Potential commercial applications of microbial biosurfactants[J]. Appl Microbiol Biot, 2000, 53(5):495-508.
[10]王佳新.外加碳源对微生物采油特性的影响[D].大连:大连工业大学,2011.
[11] MARGARITIS A, ZAJIC J E, GERSON D F, et al. Production and surface-active properties of microbial surfactants[J]. Biotechnol Bioeng,1979, 21(7):1151-1162.
[12]柴玲玲,樊国栋,葛君.无溶剂法合成食品添加剂菜籽油蔗糖酯的研究[J].中国酿造,2011,30(7):39-42.
[13] NITSCHKE M, PASTORE G M. Production and properties of a surfactant obtained from Bacillus subtilis grown on cassava waste water[J].Bioresouce Technol, 2016, 97(2):336-341.
[14]杨宁,王伟明,姚琳,等. 3,5-二硝基水杨酸法测定发酵型果露酒中总糖含量[J].中国酿造,2018,37(1):181-184.
[15] BARATHI S, VASUDEAN N. Utilization of petroleum hydrocarbons by Pseudomonas fluorescens isolated from a petroleum contaminated soil[J]. Environ Int, 2001, 26(55):413-416.
[16]刘廷丹,杨秀娟,邓君明,等.苯酚-硫酸法测定蛤蚧酒中多糖含量[J].食品工业,2018(8):298-301.
[17]李牧,杨红,刘思贤.微生物表面活性剂在稠油乳化降粘中的实验研究[J].特种油气藏,1999(4):53-58,67.
[18]曹娟,徐志辉,李凌之.生物表面活性剂的石油降解菌Acinetobacter BHSN的研究[J].生态与农村环境学报,2009,25(1):73-78.
[19]金佩强,王琪,杨克远.在MEOR过程中产生生物表面活性剂提高伊朗油藏采收率的试验方法[J].国外油油田工程,2005(10):10-14,20.
[20] MOHAMAD FULAZZAKY, DEA INDRIANI ASTUTI. Laboratory simulation of microbial enhanced oil recovery using Geobacillus toebii R-32639 isolated from the Handil reservoir[J]. RSC Adv, 2015, 5:3908-3916.