石见穿药材质量标准研究
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摘要
目的建立石见穿药材的质量标准。方法采用经验、显微鉴别法对石见穿性状、显微特征进行描述;照《中国药典》2015年版通则薄层色谱法对石见穿进行薄层色谱鉴别;照《中国药典》2015年版通则方法,测定样品的水分、总灰分、酸不溶性灰分和浸出物;采用HPLC法测定药材中迷迭香酸和丹酚酸B的含量。结果性状及鉴别方法专属性好,10批样品的水分、总灰分、酸不溶性灰分、浸出物、迷迭香酸和丹酚酸B含量的平均值分别为10.3%、7.4%、0.6%、20.8%、0.38%和0.21%。结论所建立的方法简便、准确,专属性强,可应用于石见穿药材的质量控制。
Objective To establish quality standards of Salvia chinensis herba.Methods Empirical and microscopic identification methods were adopted to observe morphological and microscopic characteristics.TLC identification and the contents of water,total ash,acid-insoluble ash and extractive were carried out according to the methods of Chinese Pharmacopoeia(2015 edition).Contents of rosmatinic acid and salvianolic B were analyzed respectively by HPLC methods.Results The established macroscopic identification,microscopic features and TLC method were proper.The average contents of water,total ash,acid-insoluble ash,water-soluble extractive and to the content of rosmatinic acid and salvianolic B were 10.3%,7.4%,0.6%,20.8%,0.38%and 0.21%,respectively.Conclusion The quality standards established based on the current research were suitable for the quality evaluation of Salvia chinensis herba.
Objective To establish quality standards of Salvia chinensis herba.Methods Empirical and microscopic identification methods were adopted to observe morphological and microscopic characteristics.TLC identification and the contents of water,total ash,acid-insoluble ash and extractive were carried out according to the methods of Chinese Pharmacopoeia(2015 edition).Contents of rosmatinic acid and salvianolic B were analyzed respectively by HPLC methods.Results The established macroscopic identification,microscopic features and TLC method were proper.The average contents of water,total ash,acid-insoluble ash,water-soluble extractive and to the content of rosmatinic acid and salvianolic B were 10.3%,7.4%,0.6%,20.8%,0.38%and 0.21%,respectively.Conclusion The quality standards established based on the current research were suitable for the quality evaluation of Salvia chinensis herba.
引文
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[10]沙秀秀,戴新新,宿树兰,等.丹参茎叶药材的质量标准研究[J].药物分析杂志,2016,36(6):1094-1100.
[11]高俊峰,丁玲,张鹏,等.高效液相色谱法测定石见穿中迷迭香酸的含量[J].中华中医药杂志,2014,29(4):1192-1193.
[12]高俊峰,丁玲,张鹏,等. HPLC法测定石见穿中丹酚酸B的含量[J].中国药房,2014,25(7):639-640.
[13]王紫燕,李春沁,穆敏婕,等. HPLC波长切换法同时测定补肾活血方中丹参素、葛根素、大豆苷和丹酚酸B的含量[J].药物分析杂志,2016,36(6):1020-1026.
[2]郝近大,谢宗万.紫参古今名实考[J].中国中药杂志, 1994,19(3):131-133.
[3]谢宗万.中华本草(第七册)[M].上海:上海科学技术出版社,1999:162-164.
[4]中国人民共和国卫生部药典委员会.中国药典(一部)[M].北京:人民卫生出版社,1978:138-139.
[5]雷涛,孟繁钦,王艳芳,等.石见穿化学成分及药理研究进展[J].中国医药导报,2010,7(29):9-10,23.
[6]高俊峰,王秀辉,张鹏,等.石见穿化学成分和药理作用研究进展[J].中国实验方剂学杂志,2013,19(12):348-351.
[7]谢永富.石见穿的研究进展[J].中国处方药,2007(9):78-78,75.
[8]山东省药品监督管理局.山东省中药材标准[S].济南:山东友谊出版社,2002:42-43.
[9]四川省食品药品监督管理局.四川省中药材标准[S].成都:四川科学技术出版社,2011:194-197.
[10]沙秀秀,戴新新,宿树兰,等.丹参茎叶药材的质量标准研究[J].药物分析杂志,2016,36(6):1094-1100.
[11]高俊峰,丁玲,张鹏,等.高效液相色谱法测定石见穿中迷迭香酸的含量[J].中华中医药杂志,2014,29(4):1192-1193.
[12]高俊峰,丁玲,张鹏,等. HPLC法测定石见穿中丹酚酸B的含量[J].中国药房,2014,25(7):639-640.
[13]王紫燕,李春沁,穆敏婕,等. HPLC波长切换法同时测定补肾活血方中丹参素、葛根素、大豆苷和丹酚酸B的含量[J].药物分析杂志,2016,36(6):1020-1026.