摘要
目的建立石见穿药材的质量标准。方法采用经验、显微鉴别法对石见穿性状、显微特征进行描述;照《中国药典》2015年版通则薄层色谱法对石见穿进行薄层色谱鉴别;照《中国药典》2015年版通则方法,测定样品的水分、总灰分、酸不溶性灰分和浸出物;采用HPLC法测定药材中迷迭香酸和丹酚酸B的含量。结果性状及鉴别方法专属性好,10批样品的水分、总灰分、酸不溶性灰分、浸出物、迷迭香酸和丹酚酸B含量的平均值分别为10.3%、7.4%、0.6%、20.8%、0.38%和0.21%。结论所建立的方法简便、准确,专属性强,可应用于石见穿药材的质量控制。
Objective To establish quality standards of Salvia chinensis herba.Methods Empirical and microscopic identification methods were adopted to observe morphological and microscopic characteristics.TLC identification and the contents of water,total ash,acid-insoluble ash and extractive were carried out according to the methods of Chinese Pharmacopoeia(2015 edition).Contents of rosmatinic acid and salvianolic B were analyzed respectively by HPLC methods.Results The established macroscopic identification,microscopic features and TLC method were proper.The average contents of water,total ash,acid-insoluble ash,water-soluble extractive and to the content of rosmatinic acid and salvianolic B were 10.3%,7.4%,0.6%,20.8%,0.38%and 0.21%,respectively.Conclusion The quality standards established based on the current research were suitable for the quality evaluation of Salvia chinensis herba.
引文
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