流感样病例咽拭子病毒核酸检测结果分析
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摘要
目的分析流感样病例咽拭子病毒核酸检测的结果。方法选择2016年12月—2018年3月在齐齐哈尔市疾病预防控制中心传染病所进行就诊的疑似流感患者60例为观察组,选择同期健康体检者60例作为对照组,进行流感样病例咽拭子病毒核酸检测。结果观察组咽拭子病毒阳性检出率为70.0%(42/60),对照组检出率为13.3%(8/60),两组之间差异有统计学意义(P<0.05)。结论采取咽拭子病毒核酸检测分析,可以有效提高病毒检出阳性率,也能够清晰监测到病毒是否存在变异,更好的了解流感的流行趋势。
Objective To analyze the results of nucleic acid detection of throat swab virus in influenza-like cases. Methods A total of 60 patients with flu suspected of being diagnosed in the First Hospital of Qiqihar City from December 2016 to March 2018 were selected as the observation group. 60 healthy controls were selected as the control group to perform influenza-like cases of throat swab virus nucleic acid. Testing. Results The positive detection rate of throat swab virus was 70.0%(42/60) in the observation group and 13.3%(8/60) in the control group. The difference between the two groups was statistically significant(P < 0.05). Conclusion The use of nucleic acid detection and analysis of the throat swab virus can effectively increase the positive rate of virus detection. It can also clearly detect the presence of mutations in the virus and better understand the trend of influenza.
Objective To analyze the results of nucleic acid detection of throat swab virus in influenza-like cases. Methods A total of 60 patients with flu suspected of being diagnosed in the First Hospital of Qiqihar City from December 2016 to March 2018 were selected as the observation group. 60 healthy controls were selected as the control group to perform influenza-like cases of throat swab virus nucleic acid. Testing. Results The positive detection rate of throat swab virus was 70.0%(42/60) in the observation group and 13.3%(8/60) in the control group. The difference between the two groups was statistically significant(P < 0.05). Conclusion The use of nucleic acid detection and analysis of the throat swab virus can effectively increase the positive rate of virus detection. It can also clearly detect the presence of mutations in the virus and better understand the trend of influenza.
引文
[1]刘亚楠,李珺,华文浩,等.两种病毒核酸提取方法在流行感冒诊断中的比较[J].中华实验和临床感染病杂志(电子版),2017,11(5):474-478.
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[12]史艳,赵冬,张国平,等.2010-2014年石家庄市流感流行季流感病毒监测结果分析[J].现代预防医学,2015,42(21):3989-3991,4004.
[2]王琼珠,孙紫虹.流感样病例咽拭子病毒核酸检测结果分析[J].中国实验诊断学,2016,20(6):978-980.
[3]石伟先,张靖,孙瑛,等.一种快速核酸提取试剂在流感病毒检测中的应用评价[J].疾病监测,2016,31(11):915-919.
[4]李珺,刘亚楠,田国保,等.临床实验室流感病毒检测的相关影响因素分析[J].国际病毒学杂志,2017,24(5):331-335.
[5]王晓东,王然,李凤焕,等.实时荧光反转录聚合酶链式反应检测甲型H1N1流感病毒RNA[J].检验医学与临床,2017,14(Z1):57-59.
[6]薛京昌,卞琛,徐晓怡.实时荧光定量PCR法在流感病毒暴发疫情病原学检测诊断中的应用[J].实用预防医学,2017,24(5):625-626.
[7]王蕾.儿童呼吸道感染标本中人偏肺病毒的分子检测与分型[J].临床医药文献电子杂志,2016,3(35):6916,6920.
[8]周高枫,王红梅,张锐沐,等.甲型流感儿童病毒核酸持续时间及其影响因素[J].中国当代儿科杂志,2016,18(12):1269-1271.
[9]颜玉炳,蒋丽娜,刘红莲,等.流感样病例中流感病毒阳性检出率的相关因素分析[J].中华疾病控制杂志,2015,19(3):310-312.
[10]马建新,温雯,黄立勇,等.北京市首例人感染H9N2禽流感病例的流行病学调查[J].国际病毒学杂志,2017,24(2):81-84.
[11]王赤华,曾勇.甲型H1N1流感病毒采用不同核酸提取方法的比较研究[J].临床和实验医学杂志,2017,16(3):230-232.
[12]史艳,赵冬,张国平,等.2010-2014年石家庄市流感流行季流感病毒监测结果分析[J].现代预防医学,2015,42(21):3989-3991,4004.