外源物质对干旱胁迫下麻黄幼苗膜质过氧化作用的影响
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摘要
以麻黄幼苗为试材,利用PEG-6000人工模拟干旱胁迫,采用盆栽灌根法,研究了腐殖酸、外源硝普钠(SNP)、甜菜碱处理下麻黄幼苗的可溶性蛋白质、丙二醛(MDA)含量及抗氧化酶活性的变化。结果表明:干旱胁迫下,麻黄幼苗MDA含量显著升高,可溶性蛋白质含量及过氧化物酶(POD)、过氧化氢酶(CAT)活性降低,超氧化物歧化酶(SOD)活性升高;对比干旱胁迫,外源物质处理下MDA含量明显降低,可溶性蛋白质及抗氧化酶活性明显增加,且随处理浓度增加均呈现先升后降的规律,随处理时间的有所增加。综合表明,2.5mmo1·L~(-1)的甜菜碱处理10d为缓解氧化损伤效果的最佳处理,此外100mL·L~(-1)的腐植酸、0.25mmo1·L~(-1)的SNP也有效地缓解了麻黄干旱胁迫,3种外源物质不同程度地提高了麻黄幼苗的抗旱能力,为干旱半干旱地区麻黄的推广栽培提供了依据。
Ephedra sinica seedlings were used as the experimental materials,drought stress was simulated with PEG-6000,the plate culture with irrigation method was used to determine contents of solue protein,MDA and antioxidant enzyme activity in Ephedra sinica seedlings under HA,exogenous SNP,betaine.The results showed that the contents of MDA raised significantly,the contents of solue protein,activities of POD and CAT reduced,while the activity of SOD increased;in contrast to drought stress,the contents of MDA descended obviously,the contents of solue protein and antioxidant enzyme activity increased significantly under treatments of exogenous substances.The above indexes raised firstly,then decreased with the increase in concentration of exogenous substances and increased gradually at last during treating time.The comprehensive results showed that the treatments of 2.5 mmo1·L~(-1) betaine and 10 days treatment time was the best to relief membrane lipid peroxidation,meanwhile the treatment of 100mL·L~(-1) HA and 0.25mmo1·L~(-1) SNP relieved the drought stresses effectively,the drought resistance was advanced in some degree of Ephedra sinicaseedlings under three exogenous substances,it provided a basis for extend culture of Ephedra sinicain arid and semi-arid areas.
Ephedra sinica seedlings were used as the experimental materials,drought stress was simulated with PEG-6000,the plate culture with irrigation method was used to determine contents of solue protein,MDA and antioxidant enzyme activity in Ephedra sinica seedlings under HA,exogenous SNP,betaine.The results showed that the contents of MDA raised significantly,the contents of solue protein,activities of POD and CAT reduced,while the activity of SOD increased;in contrast to drought stress,the contents of MDA descended obviously,the contents of solue protein and antioxidant enzyme activity increased significantly under treatments of exogenous substances.The above indexes raised firstly,then decreased with the increase in concentration of exogenous substances and increased gradually at last during treating time.The comprehensive results showed that the treatments of 2.5 mmo1·L~(-1) betaine and 10 days treatment time was the best to relief membrane lipid peroxidation,meanwhile the treatment of 100mL·L~(-1) HA and 0.25mmo1·L~(-1) SNP relieved the drought stresses effectively,the drought resistance was advanced in some degree of Ephedra sinicaseedlings under three exogenous substances,it provided a basis for extend culture of Ephedra sinicain arid and semi-arid areas.
引文
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[16]毛亚斌,魏小红.外源NO对干旱胁迫下板蓝根叶片氧化损伤的保护作用[J].草业科学,2010,27(6):97-101.
[17]王芳,赵有军,王汉宁.外源NO对干旱胁迫下玉米幼苗膜质过氧化的调节效应[J].干旱地区农业研究,2015,3(9):75-79.
[18]杨丽文,李敬蕊,高洪波,等.干旱胁迫下外源物质对番茄幼苗活性氧代谢及光合作用的影响[J].河北农业大学学报,2012,35(2):18-24.
[2]WHITFORD W,WADE E L.Ecology of desert systems[M].San Diego,California,USA:Academic Press,2002.
[3]吴文芳,李隆云.药用植物抗旱研究进展[J].安徽农业科学,2011,39(24):14647-14648.
[4]何习斌.麻黄种子育苗技术研究[D].杨凌:西北农林科技大学,2007.
[5]WANG J,PING H E,ZHANG C P,et al.Effect of exogenous nitriv oxide donor SNP,betaine and humic acid on physiological characters of Celosia cristataseedlings under drought stress[J].Journal of Southwest University,2014,36(4):14-21.
[6]回振龙,李自龙,李朝周,等.外源NO供体SNP对PEG模拟干旱胁迫下高羊茅种子萌发及幼苗抗性生理的影响[J].华北农学报,2013,28(4):86-92.
[7]曹丽,回振龙,魏小红,等.SNP对PEG模拟干旱胁迫下早熟禾种子萌发及幼苗抗性的影响[J].甘肃农业大学学报,2013(5):100-106.
[8]张红敏,李姣姣,黑刚刚,等.外源甜菜碱处理对干旱胁迫下半夏氮代谢及相关酶活性的影响[J].草业学报,2014,23(4):229-236.
[9]VELIKOVA V,YORDANOV I,EDREVA A.Oxidative stress and some antioxidant systems science in acid rain-treated bean plants protective role of exogenous polyamines[J].Plant Science,2000,151:59-66.
[10]张以顺,黄霞,陈云凤.植物生理学实验教程[M].北京:高等教育出版社,2009:136-142.
[11]张美俊,杨武德,乔治军,等.不同糜子品种萌发期对干旱胁迫的响应及抗旱性评价[J].草地学报,2013,21(2):302-307.
[12]ASHRAF M,FOOLAD M R.Roles of glycine betaine and proline in improving plant abiotic stress resistance[J].Environmental and Experimental Botany,2007,59(2):206-216.
[13]马明莉,周文波,钟雪侮,等.外源水杨酸对干旱胁迫下甘蓝型油菜幼苗生理特性的影响[J].江苏农业科学,2015,43(6):84-87.
[14]WU J,YANG H,LUO Y,et al.Physiological effects of sodium nitroprusside on arabidopsis under drought stress[J].Acta Agriculturae Boreali-Occidentalis Sinica,2010,19(12):94-97.
[15]SHEHAB G G,AHMED O K,EI-BELTAGI H S.Effects of various chemical agents for alleviation of drought stress in rice plants(Oryza sativa L.)[J].Notulae Botanicae Horti Agrobotanici Cluj-Napoca,2010,38(1):139-148.
[16]毛亚斌,魏小红.外源NO对干旱胁迫下板蓝根叶片氧化损伤的保护作用[J].草业科学,2010,27(6):97-101.
[17]王芳,赵有军,王汉宁.外源NO对干旱胁迫下玉米幼苗膜质过氧化的调节效应[J].干旱地区农业研究,2015,3(9):75-79.
[18]杨丽文,李敬蕊,高洪波,等.干旱胁迫下外源物质对番茄幼苗活性氧代谢及光合作用的影响[J].河北农业大学学报,2012,35(2):18-24.