金寨红阳猕猴桃提取物抗肿瘤及抑菌活性研究
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摘要
[目的]分析金寨红阳猕猴桃提取物抗肿瘤及抑菌活性。[方法]采用水提法、醇提法提取金寨红阳猕猴桃活性物质。通过提取物对SH-SY5Y、HELA和MCF-7这3种肿瘤细胞形态结构的破坏以及生长抑制,测定其抗肿瘤活性。通过提取物对大肠杆菌、枯草芽孢杆菌、黑曲霉以及酵母菌的生长抑制作用,分析其抑菌功能。[结果]金寨红阳猕猴桃提取物能破坏3种肿瘤细胞的细胞形态,当添加量为20.0μL时,对SH-SY5Y的抑制率为38%±6%,对HELA的抑制率为25%±5%,对MCF-7的抑制率为24%±3%。提取物添加量为40.0μL时,可完全抑制大肠杆菌及枯草芽孢杆菌的生长。提取物添加量为80.0μL时对黑曲霉及酵母菌没有明显的抑制作用。[结论]金寨红阳猕猴桃提取物能抑制肿瘤细胞及细菌的生长,可用于保健品及药物的开发利用。
[Objective] The research aimed to study the antitumor and antimicrobial activity of Jinzhai Hongyang kiwi fruit extract. [Method]The active substances of Jinzhai Hongyang kiwi fruit were extracted by distilled water and ethanol respectively. The antitumor activity of the extract was measured by the destruction of the cell morphology and growth inhibition against SH-SY5Y,HELA and MCF-7. The growth inhibitory of the extract was also analyzed against Escherichia coli,Bacillus subtilis,Aspergillus niger and Saccharomyces. [Result] The kiwi fruit extract could destroy the morphology of three kinds of tumor cells. The inhibition rate of the extract against SH-SY5Y was 38% ±6% at the additive amount of 20.0 μL,and it was 25% ±5% against HELA,which was 24% ±3% against MCF-7. The growth of escherichia coli and bacillus subtilis could be completely inhibited by the extract at the additive amount of 40.0 μL. The extract had no significant inhibition against aspergillus Niger and Saccharomyces at the additive amount of 80.0 μL. [Conclusion]Jinzhai Hongyang kiwi fruit extract can inhibit the growth of tumor cells and bacteria,and it can be used for the development of health care products and drugs.
[Objective] The research aimed to study the antitumor and antimicrobial activity of Jinzhai Hongyang kiwi fruit extract. [Method]The active substances of Jinzhai Hongyang kiwi fruit were extracted by distilled water and ethanol respectively. The antitumor activity of the extract was measured by the destruction of the cell morphology and growth inhibition against SH-SY5Y,HELA and MCF-7. The growth inhibitory of the extract was also analyzed against Escherichia coli,Bacillus subtilis,Aspergillus niger and Saccharomyces. [Result] The kiwi fruit extract could destroy the morphology of three kinds of tumor cells. The inhibition rate of the extract against SH-SY5Y was 38% ±6% at the additive amount of 20.0 μL,and it was 25% ±5% against HELA,which was 24% ±3% against MCF-7. The growth of escherichia coli and bacillus subtilis could be completely inhibited by the extract at the additive amount of 40.0 μL. The extract had no significant inhibition against aspergillus Niger and Saccharomyces at the additive amount of 80.0 μL. [Conclusion]Jinzhai Hongyang kiwi fruit extract can inhibit the growth of tumor cells and bacteria,and it can be used for the development of health care products and drugs.
引文
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[7]MONTEFIORI M,MCGHIE T K,COSTA G,et al.Pigments in the fruit of red-fleshed kiwifruit(Actinidia chinensis and Actinidia deliciosa)[J].Journal of agricultural and food chemistry,2005,53(24):9526-9530.
[8]徐春华.MTT法检测大豆异黄酮对癌细胞的生长抑制作用[J].中央民族大学学报(自然科学版),2012,21(1):58-62.
[2]康大力,张洪利.猕猴桃属植物化学成分及其生物活性研究进展[J].中成药,2008,30(1):116-119.
[3]朱振宁.美味猕猴桃根抑菌活性及化学成分研究[D].杨凌:西北农林科技大学,2006.
[4]LEE Y C,KIM S H,SEO Y B,et al.Inhibitory effects of Actinidia polygama extract and cyclosporine A on OVA-induced eosinophilia and bronchial hyperresponsiveness in a murine model of asthma[J].International immunopharm acology,2006,6(4):703-713.
[5]王华,曹婧,翟丽娟,等.猕猴桃果肉提取物抗氧化活性研究[J].华北农学报,2013,28(2):144-149.
[6]马思远,黄初升,刘红星,等.中华猕猴桃根化学成分及药理活性的研究进展[J].广西师范学院学报(自然科学版),2016,33(4):57-63.
[7]MONTEFIORI M,MCGHIE T K,COSTA G,et al.Pigments in the fruit of red-fleshed kiwifruit(Actinidia chinensis and Actinidia deliciosa)[J].Journal of agricultural and food chemistry,2005,53(24):9526-9530.
[8]徐春华.MTT法检测大豆异黄酮对癌细胞的生长抑制作用[J].中央民族大学学报(自然科学版),2012,21(1):58-62.