联苯吡菌胺原药的高效液相色谱分析
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摘要
[目的]开发一种联苯吡菌胺原药含量测定的高效液相色谱分析方法。[方法]采用Waters e2695型高效液相色谱仪,依利特C18柱进行分离和定量分析,流动相为甲醇-水(体积比69∶31),流速为1.0 m L/min,柱温为30℃,检测波长为259 nm。[结果]联苯吡菌胺在0.0396～0.7920 g/L的质量浓度范围内线性关系良好,相关系数为0.9999;精密度及重复性试验的RSD值分别为1.17%和0.54%,平均回收率为99.88%。[结论]该方法高效、灵敏、准确,可用于联苯吡菌胺的定量分析。
[Aims] A HPLC method for the determination of bixafen TC was developed. [Methods] Waters Alliance e2695 HPLC system and Eliet C18 column were adopted for the separation and quantification. The mobile phase was methanol and water(69:31, by vol) at the flow rate of 1.0 m L/min and the column temperature of 30 ℃. The detection wavelength was 259 nm. [Results] There was a good linear relationship between the concentration and the peak area of bixafen in the concentration range of 0.0396-0.7920 g/L, and the linear regression coefficient was 0.9999. The relative standard deviations of precision and repeatability test were 1.17 and 0.54%, respectively. The average recovery was99.88%. [Conclusions] The method has high column efficiency, sensitivity and accuracy, and could be used for the quantitative analysis of bixafen TC.
[Aims] A HPLC method for the determination of bixafen TC was developed. [Methods] Waters Alliance e2695 HPLC system and Eliet C18 column were adopted for the separation and quantification. The mobile phase was methanol and water(69:31, by vol) at the flow rate of 1.0 m L/min and the column temperature of 30 ℃. The detection wavelength was 259 nm. [Results] There was a good linear relationship between the concentration and the peak area of bixafen in the concentration range of 0.0396-0.7920 g/L, and the linear regression coefficient was 0.9999. The relative standard deviations of precision and repeatability test were 1.17 and 0.54%, respectively. The average recovery was99.88%. [Conclusions] The method has high column efficiency, sensitivity and accuracy, and could be used for the quantitative analysis of bixafen TC.
引文
[1]罗梁峰.新一代琥珀酸脱氢抑制酶联苯吡菌胺[J].世界农药,2018, 40(1):63-64.
[2]李良孔,袁善奎,潘洪玉,等.琥珀酸脱氢酶抑制剂类(SDHIs)杀菌剂及其抗性研究进展[J].农药, 2011, 50(3):165-169.
[3]柏亚罗,顾林玲. 2017年全球登记或上市的农药新产品[J].现代农药, 2018, 17(1):8-15.
[4]仇是胜,柏亚罗.琥珀酸脱氢酶抑制剂类杀菌剂的研发进展(Ⅱ)[J].现代农药, 2015, 14(1):1-8.
[5]柏亚罗.拜耳富美实联袂打造重磅杀菌剂联苯吡菌胺[EB/OL].(2016-11-04)[2018-7-19]. Http://www.agroinfo.com.cn/other_detail_3428.html.
[2]李良孔,袁善奎,潘洪玉,等.琥珀酸脱氢酶抑制剂类(SDHIs)杀菌剂及其抗性研究进展[J].农药, 2011, 50(3):165-169.
[3]柏亚罗,顾林玲. 2017年全球登记或上市的农药新产品[J].现代农药, 2018, 17(1):8-15.
[4]仇是胜,柏亚罗.琥珀酸脱氢酶抑制剂类杀菌剂的研发进展(Ⅱ)[J].现代农药, 2015, 14(1):1-8.
[5]柏亚罗.拜耳富美实联袂打造重磅杀菌剂联苯吡菌胺[EB/OL].(2016-11-04)[2018-7-19]. Http://www.agroinfo.com.cn/other_detail_3428.html.