HPLC法同时测定通脉口服液中9种甜味剂和防腐剂的研究
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摘要
目的:建立通脉口服液中苯甲酸、山梨酸钾、安赛蜜、糖精钠、阿斯巴甜、对羟基苯甲酸酯类等9种甜味剂及防腐剂的高效液相测定方法,为有效控制成方制剂的安全性提供参考。方法:采用高效液相色谱法,二极管阵列检测器,色谱柱为Thermo HYPERSIL GOLD Aq C18(4.6 mm×150 mm,5μm),流动相为乙腈和0.02 mol·L-1磷酸二氢铵(用三乙胺调pH值为5.5)梯度洗脱。结果:9种成分线性关系良好,均在线性范围内,10批通脉口服液样品中的山梨酸钾含量均在可控范围内。结论:该方法专属性强,简单易行,适用于通脉口服液中9种甜味剂和防腐剂的测定。
Objective:To establish a method for the determination of 9 sweeteners and preservatives in Tongmai oral liquid,such as benzoic acid,potassium sorbate,acesulfame,saccharin sodium,aspartame and p-hydroxybenzoate,by high performance liquid chromatography(HPLC),and to provide reference for the effective control of the safety of prescription preparations.Methods:HPLC with diode array detector and Thermo HYPERSIL GOLD Aq C18(4.6 mm×150 mm,5 μm) was used.The mobile phase was acetonitrile and 0.02 mol·L~(-1) ammonium dihydrogen phosphate(adjusted by triethylamine to pH value by 5.5) gradient elution.Results:The linearity of the nine components was good,and the content of potassium sorbate in 10 batches of Tongmai oral liquid samples was within the controllable range.Conclusion:The method is simple and specific,and suitable for the determination of 9 sweeteners and preservatives in Tongmai oral liquid.
Objective:To establish a method for the determination of 9 sweeteners and preservatives in Tongmai oral liquid,such as benzoic acid,potassium sorbate,acesulfame,saccharin sodium,aspartame and p-hydroxybenzoate,by high performance liquid chromatography(HPLC),and to provide reference for the effective control of the safety of prescription preparations.Methods:HPLC with diode array detector and Thermo HYPERSIL GOLD Aq C18(4.6 mm×150 mm,5 μm) was used.The mobile phase was acetonitrile and 0.02 mol·L~(-1) ammonium dihydrogen phosphate(adjusted by triethylamine to pH value by 5.5) gradient elution.Results:The linearity of the nine components was good,and the content of potassium sorbate in 10 batches of Tongmai oral liquid samples was within the controllable range.Conclusion:The method is simple and specific,and suitable for the determination of 9 sweeteners and preservatives in Tongmai oral liquid.
引文
[1]谢兆辉,李学贵,许褆森.防腐剂苯甲酸(钠)测定方法的研究进展[J].粮食与油脂,2015,28(3):6-8.
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[3]李薇.梯度洗脱HPLC法测定盐酸氨溴索口服液中甜味剂含量[J].药物分析杂志,2012,32(12):2208.
[4]孙蒙,闫小玉,毕开顺,等.HPLC测定11种中药口服液中6种防腐剂的含量[J].中国实验方剂学杂志,2012,18(3):76-79.
[5]陈安东,吴兆伟,余倩,等.GC/MS法同时分析液体制剂及口服液型保健品中31种防腐剂和抗氧化剂[J].质谱学报,2014,35(5):438-446.
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[7]田海英,宋金勇,王宏伟,等.RP-HPLC法测定烟用香精香料中的7种防腐剂[J].烟草化学,2011,2:49.
[8]曾延灵,朴杰.超高效液相色谱法测定冷面汤中山梨酸、苯甲酸、安赛蜜、糖精钠含量[J].中国卫生工程学,2018,17(6):888-890.
[9]李花觉.超高效液相色谱法检测饮料中山梨酸、苯甲酸、糖精钠、咖啡因、安赛蜜[J].分析检测,2018,21(43):137-139.
[10]罗晶,施法,陈晓辉,等.HPLC法同时测定抗病毒口服液中4种防腐剂[J].西北药学杂志,2012,27(1):36-38.
[11]黎艳刚,段峰,郭丽蓉,等.HPLC测定11种中药口服液中6种防腐剂的含量布洛芬口服液中苯甲酸钠、安赛蜜含量[J].中国实验方剂学杂志,2013,19(8):69-71.
[12]闫妍,刘永明,朱晓琼.HPLC法同时测定对羟基苯甲酸酯类及对羟基苯甲酸含量[J].中国药物评价,2014,31(2):83-85.
[13]凌云,储晓刚,张峰,等.超高效液相色谱-串联质谱法同时测定调味料中的17种防腐剂和抗氧化剂[J].色谱,2011,29(8):723-730.
[14]文玉辉,郭璞.建立口服液制剂中苯甲酸、山梨酸、糖精钠的含量检测方法[J].中国药事,2015,29(3):313.
[15]戚繁,王少伟,颜春荣,等.高效液相色谱法同时测定甜油中苯甲酸、山梨酸、安赛蜜、糖精钠与脱氢乙酸[J].分析检测,2018,20(33):101-104.
[16]吴镁春,王佳欢.UPLC法检测四种口服液体中苯甲酸钠的含量[J].广州化工,2018,46(23):113-115.
[2]牛娜.食品防腐剂检验检测技术的研究现状及进展[J].行业科技文苑,2018,34(29):68-69.
[3]李薇.梯度洗脱HPLC法测定盐酸氨溴索口服液中甜味剂含量[J].药物分析杂志,2012,32(12):2208.
[4]孙蒙,闫小玉,毕开顺,等.HPLC测定11种中药口服液中6种防腐剂的含量[J].中国实验方剂学杂志,2012,18(3):76-79.
[5]陈安东,吴兆伟,余倩,等.GC/MS法同时分析液体制剂及口服液型保健品中31种防腐剂和抗氧化剂[J].质谱学报,2014,35(5):438-446.
[6]国家药典委员会.中华人民共和国药典(四部)[S].北京:中国医药科技出版社,2015:26.
[7]田海英,宋金勇,王宏伟,等.RP-HPLC法测定烟用香精香料中的7种防腐剂[J].烟草化学,2011,2:49.
[8]曾延灵,朴杰.超高效液相色谱法测定冷面汤中山梨酸、苯甲酸、安赛蜜、糖精钠含量[J].中国卫生工程学,2018,17(6):888-890.
[9]李花觉.超高效液相色谱法检测饮料中山梨酸、苯甲酸、糖精钠、咖啡因、安赛蜜[J].分析检测,2018,21(43):137-139.
[10]罗晶,施法,陈晓辉,等.HPLC法同时测定抗病毒口服液中4种防腐剂[J].西北药学杂志,2012,27(1):36-38.
[11]黎艳刚,段峰,郭丽蓉,等.HPLC测定11种中药口服液中6种防腐剂的含量布洛芬口服液中苯甲酸钠、安赛蜜含量[J].中国实验方剂学杂志,2013,19(8):69-71.
[12]闫妍,刘永明,朱晓琼.HPLC法同时测定对羟基苯甲酸酯类及对羟基苯甲酸含量[J].中国药物评价,2014,31(2):83-85.
[13]凌云,储晓刚,张峰,等.超高效液相色谱-串联质谱法同时测定调味料中的17种防腐剂和抗氧化剂[J].色谱,2011,29(8):723-730.
[14]文玉辉,郭璞.建立口服液制剂中苯甲酸、山梨酸、糖精钠的含量检测方法[J].中国药事,2015,29(3):313.
[15]戚繁,王少伟,颜春荣,等.高效液相色谱法同时测定甜油中苯甲酸、山梨酸、安赛蜜、糖精钠与脱氢乙酸[J].分析检测,2018,20(33):101-104.
[16]吴镁春,王佳欢.UPLC法检测四种口服液体中苯甲酸钠的含量[J].广州化工,2018,46(23):113-115.